Exposure to elevated ozone levels differentially affects the antioxidant capacity and the redox homeostasis of two subtropical Phaseolus vulgaris L. varieties

Caregnato, Fernanda Freitas; Bortolin, Rafael Calixto; Divan, Armando Molina; Moreira, José Cláudio Fonseca

doi:10.1016/j.chemosphere.2013.04.084

Cited by 52 publications

(21 citation statements)

References 93 publications

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“…Carbohydrates may take part in plant antioxidant defense system along with nonenzymatic and enzymatic components of the ascorbate-gluthatione cycle (Jaleel et al 2009). Ascorbate peroxidase (APX) plays a crucial role in detoxifying hydrogen peroxide (H 2 O 2 ) among the enzymatic antioxidants in such cycle using reduced ascorbate as substrate (Caregnato et al 2013). APX activity increases in response to a variety of biotic and abiotic stresses in different plant species, including the exposure to ozone (Asada 1999).…”

Section: Introductionmentioning

confidence: 99%

Fructans, ascorbate peroxidase, and hydrogen peroxide in ryegrass exposed to ozone under contrasting meteorological conditions

Pasqualetti

Sandrin

Pedroso

et al. 2015

Environ Sci Pollut Res

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Ozone (O3) is the most abundant tropospheric oxidant as well as an important component of photochemical pollution. Once inside the plant, ozone can produce reactive oxygen species that change the antioxidative pool and the carbohydrate metabolism. The current study aimed to analyze whether the contents and the composition of the fructan, the ascorbate peroxidase activity, and the H2O2 accumulation were changed in Lolium multiflorum ssp. italicum cv. Lema plants as response to short-term exposure to ozone and/or to different meteorological conditions, in two contrasting seasons (winter and summer). Results showed that higher solar radiation tends to decrease fructose content and, along with temperature, increases the ascorbate peroxidase (APX) activity. Such activity and levels of fructans practically did not vary during the time the experiment was being done, but APX daylight variation was modified by the ozone. Thus, the higher levels of this pollutant decreased the APX activity and increased fructose content, as well as changed the size of the fructan chains. Hydrogen peroxide (H2O2) accumulation was higher in plants that were fumigated with ozone when compared to the control, and it decreased throughout the day. As a conclusion, fructan contents increased when the APX activity decreased. It suggested that fructans could also help the defense system when there is a reduction on the APX activity in the plant.

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Section: Introductionmentioning

confidence: 99%

Fructans, ascorbate peroxidase, and hydrogen peroxide in ryegrass exposed to ozone under contrasting meteorological conditions

Pasqualetti

Sandrin

Pedroso

et al. 2015

Environ Sci Pollut Res

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“…Asc biosynthesis is also dependent on photosynthetic electron transport (Kiyota et al, 2006). Under stress conditions, including UV-B (Gao & Zhang, 2008), ozone (Caregnato et al, 2013), salt (Huang et al, 2005) and high-light stress (Dowdle et al, 2007;Bulley & Laing, 2016), a two-to three-fold increase in Asc concentrations can be observed on the timescale of days.…”

Section: Introductionmentioning

confidence: 99%

Regulation of ascorbate biosynthesis in green algae has evolved to enable rapid stress‐induced response via the VTC2 gene encoding GDP‐l‐galactose phosphorylase

et al. 2017

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Ascorbate (vitamin C) plays essential roles in stress resistance, development, signaling, hormone biosynthesis and regulation of gene expression; however, little is known about its biosynthesis in algae. In order to provide experimental proof for the operation of the Smirnoff-Wheeler pathway described for higher plants and to gain more information on the regulation of ascorbate biosynthesis in Chlamydomonas reinhardtii, we targeted the VTC2 gene encoding GDP-l-galactose phosphorylase using artificial microRNAs. Ascorbate concentrations in VTC2 amiRNA lines were reduced to 10% showing that GDP-l-galactose phosphorylase plays a pivotal role in ascorbate biosynthesis. The VTC2 amiRNA lines also grow more slowly, have lower chlorophyll content, and are more susceptible to stress than the control strains. We also demonstrate that: expression of the VTC2 gene is rapidly induced by H O and O resulting in a manifold increase in ascorbate content; in contrast to plants, there is no circadian regulation of ascorbate biosynthesis; photosynthesis is not required per se for ascorbate biosynthesis; and Chlamydomonas VTC2 lacks negative feedback regulation by ascorbate in the physiological concentration range. Our work demonstrates that ascorbate biosynthesis is also highly regulated in Chlamydomonas albeit via mechanisms distinct from those previously described in land plants.

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“…Many studies have shown that ozone treatment reduces the levels of pigments, such as chlorophylls and carotenoids, involved in photosynthesis [20][21][22][23][24]. The decrease of chlorophyll concentration is considered to be a secondary response to ozone exposure [25].…”

Section: Introductionmentioning

confidence: 99%

The effect of tropospheric ozone on flavonoids and pigments content in common buckwheat cotyledons

Dębski

Wiczkowski

Szawara-Nowak

et al. 2017

Ecological Chemistry and Engineering S

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Tropospheric ozone forms in photochemical reactions or by refuse burning and combustion of exhaust gases from engines, and during some industrial processes. The mean ambient ozone concentration doubled during the last century, and in many urban areas has reached the phytotoxic level. In the present study, there was determined the effect of ozone fumigation on levels of individual flavonoids, chlorophylls, carotenoids and total phenols in the cotyledons of four common buckwheat cultivars (Hruszowska, Panda, Kora and Red Corolla). Six-day-old buckwheat seedlings were grown in controlled conditions and treated with an elevated dose of ozone (391 µg · m -3 ) during 5 days for 1 h each day. After the experiment, the cotyledons of the seedlings were analysed for individual flavonoids, chlorophylls, carotenoids and total phenols. Shoot elongation was also measured. Individual types of flavonoids in buckwheat cotyledons were found to respond to an elevated ozone dose in various ways. The response was also dependent on the cultivar evaluated. In the cotyledons of ozonized buckwheat seedlings, contents of C-glucosides of luteolin and apigenin decreased or did not change depending on the cultivar examined. In the case of flavonols, the contents of quercetin-3-O-rhamnosyl-galactoside and rutin (quercetin-3-O-rhamnosyl-glucoside) were markedly reduced in most cultivars. O3 had no effect on the level of anthocyanins and chlorophylls but it decreased carotenoids, and tended to inhibit buckwheat growth. In conclusion, a thesis can be formulated that, due to high reduction in important flavonoids, an elevated level of ambient ozone decreases the nutritional value of common buckwheat seedlings.

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Exposure to elevated ozone levels differentially affects the antioxidant capacity and the redox homeostasis of two subtropical Phaseolus vulgaris L. varieties

Cited by 52 publications

References 93 publications

Fructans, ascorbate peroxidase, and hydrogen peroxide in ryegrass exposed to ozone under contrasting meteorological conditions

Fructans, ascorbate peroxidase, and hydrogen peroxide in ryegrass exposed to ozone under contrasting meteorological conditions

Regulation of ascorbate biosynthesis in green algae has evolved to enable rapid stress‐induced response via the VTC2 gene encoding GDP‐l‐galactose phosphorylase

The effect of tropospheric ozone on flavonoids and pigments content in common buckwheat cotyledons

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