Exposure to elevated glucose concentrations alters the metabolomic profile of bovine blastocysts

Uhde, Karen; Tol, Helena T A van; Stout, T.A.E.

doi:10.1371/journal.pone.0199310

Cited by 11 publications

(11 citation statements)

References 54 publications

(55 reference statements)

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“…Interestingly, maltose has been shown to inhibit the binding of bovine and porcine spermatozoa to oviduct epithelial cell carbohydrates, a process allowing sperm storage and the delivery of capacitated spermatozoa to the site of fertilization [44,45]. The low levels of sources of glucose in oEVs in the post-ovulatory period are also concordant with the fact that exposure of the early bovine embryo to high amounts of glucose in vitro disturb embryo development and metabolism [46,47].…”

Section: Discussionmentioning

confidence: 97%

Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle

Gatien

Mermillod

Tsikis

et al. 2019

IJMS

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Oviductal extracellular vesicles (oEVs) have been proposed as key modulators of gamete/embryo maternal interactions. The aim of this study was to examine the metabolite content of oEVs and its regulation across the estrous cycle in cattle. Oviductal EVs were isolated from bovine oviducts ipsilateral and contralateral to ovulation at four stages of the estrous cycle (post-ovulatory stage, early and late luteal phases, and pre-ovulatory stage). The metabolomic profiling of EVs was performed by proton nuclear magnetic resonance spectroscopy (NMR). NMR identified 22 metabolites in oEVs, among which 15 were quantified. Lactate, myoinositol, and glycine were the most abundant metabolites throughout the estrous cycle. The side relative to ovulation had no effect on the oEVs' metabolite concentrations. However, levels of glucose-1-phosphate and maltose were greatly affected by the cycle stage, showing up to 100-fold higher levels at the luteal phase than at the peri-ovulatory phases. In contrast, levels of methionine were significantly higher at peri-ovulatory phases than at the late-luteal phase. Quantitative enrichment analyses of oEV-metabolites across the cycle evidenced several significantly regulated metabolic pathways related to sucrose, glucose, and lactose metabolism. This study provides the first metabolomic characterization of oEVs, increasing our understanding of the potential role of oEVs in promoting fertilization and early embryo development.

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Section: Discussionmentioning

confidence: 97%

Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle

Gatien

Mermillod

Tsikis

et al. 2019

IJMS

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“…Many studies reported that fatty acids are ligands for PPARs to transcribe genes for mitochondrial fatty acid oxidation, and PPAR inhibition reduces fatty acid oxidation as well as lipolysis [8,42]. Furthermore, elevated concentrations of non-esterified fatty acids and glucose enhance lipid contents and reduce lipolysis and fatty acid β-oxidation [43,44]. Increased lipid contents were correlated with apoptosis in blastocysts [38].…”

Section: Discussionmentioning

confidence: 99%

The PPARδ Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos

Idrees

Sheikh

et al. 2019

IJMS

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The PPARs (peroxisome proliferator-activated receptors) play critical roles in the regulation of lipid and glucose metabolism. PPARδ, a member of the PPARs family, is associated with decreased susceptibility to ectopic lipid deposition and is implicated in the regulation of mitochondrial processes. The current study aimed to determine the role of PPARδ in fatty acid β-oxidation and its influence on PEPCK for the lipogenic/lipolytic balance during in vitro bovine oocyte maturation and embryo development. Activation of PPARδ by GW501516, but not 2-BP, was indicated by intact embryonic PEPCK (cytosolic) and CPT1 expression and the balance between free fatty acids and mitochondrial β-oxidation that reduced ROS and inhibited p-NF-κB nuclear localization. Genes involved in lipolysis, fatty acid oxidation, and apoptosis showed significant differences after the GW501516 treatment relative to the control- and 2-BP-treated embryos. GSK3787 reversed the PPARδ-induced effects by reducing PEPCK and CPT1 expression and the mitochondrial membrane potential, revealing the importance of PPARδ/PEPCK and PPARδ/CPT1 for controlling lipolysis during embryo development. In conclusion, GW501516-activated PPARδ maintained the correlation between lipolysis and lipogenesis by enhancing PEPCK and CPT1 to improve bovine embryo quality.

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“…We could not elucidate the effects of glucose and albumin levels on the embryo recovery rate after multiple superovulations with AI, but we proved that glucose level affects embryonic development in vitro . A high glucose concentration in the embryo culture media deleteriously affected the blastocyst developmental rate compared with a low glucose concentration in bovine [ 30 ] and mouse [ 31 ]. Bovine serum albumin contains numerous amino acids, and the addition of albumin to a culture medium has a positive effect on embryonic development in vitro [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate

Kang¹,

Kim²,

Han³

et al. 2020

J Anim Sci Technol

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Here, we investigated the effects of different nutrient requirements (NR) on blood metabolites, transferable embryo number after multiple superovulations with artificial insemination (AI), body condition score (BCS), and estrus cycle in Hanwoo cow. Nineteen Hanwoo cows were randomly divided into three groups (80%, 100%, and 120% NR, containing 6, 8, and 5 individuals, respectively) and fed based on the NR. In experiment 1, glucose, total cholesterol, triglyceride, blood urea nitrogen (BUN), aspartate aminotransferase (AST), alanine aminotransferase (ALT), non-esterified fatty acids (NEFA), albumin (ALB), and total protein (TP) were analyzed. In experiment 2, total number of recovered embryos and transferable embryos was examined after embryo recovery and multiple superovulations with AI. In experiment 3, body weight, BCS, and estrus cycle were examined. In experiment 1, total cholesterol was significantly different among the 80%, 100%, and 120% NR groups (126.5 ± 3.3, 152.6 ± 2.4, and 177.4 ± 1.8 mg/dL, respectively, p < 0.05). The triglyceride and BUN levels in the 120% NR group were significantly higher than those in the 80% and 100% groups ( p < 0.05). The NEFA levels were significantly different among the 80%, 100%, and 120% NR groups (440.5 ± 18.2, 318.5 ± 23.1, and 195.1 ± 8.5 ЧEq/L, respectively, p < 0.05). The AST and TP levels in the 80% NR group were significantly lower than those in the 100% and 120% NR groups ( p < 0.05). In experiment 2, the 120% NR group showed a higher percentage of transferable embryos than the 80% and 100% groups ( p < 0.01). The mean body weight and BCS among the 80%, 100%, and 120% NR groups were significantly different ( p < 0.05). The estrus cycle in the 80% NR group was delayed compared with the 100% and 120% NR groups (20.8 ± 0.2 and 21.2 ± 0.5 days, respectively). In conclusion, the blood metabolic tests proved that Hanwoo cows with 120% NR can produce a large number of transferable embryos. Thus, 120% NR is the appropriate feeding level for this type of cows as it results in the production of a large number of transferable embryos by multiple superovulations with AI.

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Exposure to elevated glucose concentrations alters the metabolomic profile of bovine blastocysts

Cited by 11 publications

References 54 publications

Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle

Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle

The PPARδ Agonist GW501516 Improves Lipolytic/Lipogenic Balance through CPT1 and PEPCK during the Development of Pre-Implantation Bovine Embryos

Nutrient requirements in Hanwoo cows with artificial insemination: effects on blood metabolites and embryo recovery rate

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