Exposure to D2-like dopamine receptor agonists inhibits swimming in Daphnia magna

Barrozo, Enrico R.; Fowler, D.; Beckman, Matthew L.

doi:10.1016/j.pbb.2015.08.010

Cited by 19 publications

(10 citation statements)

References 37 publications

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“…Daphnia are keystone zooplankton in freshwater ecosystems and model organisms for studying anthropogenic and natural stressors in these ecosystems [30]. They have also been used in studies of the dopamine signalling system [31,32]. We hypothesize that D. magna with natural dopamine levels will have life-history reaction norms that approach optimality with respect to fitness in response to food abundance, and that disruption of these levels will lead to a change in the response to food abundance and hence the slopes of these reaction norms.…”

Section: Introductionmentioning

confidence: 99%

Dopamine mediates life-history responses to food abundance in Daphnia

et al. 2020

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Expression of adaptive reaction norms of life-history traits to spatio-temporal variation in food availability is crucial for individual fitness. Yet little is known about the neural signalling mechanisms underlying these reaction norms. Previous studies suggest a role for the dopamine system in regulating behavioural and morphological responses to food across a wide range of taxa. We tested whether this neural signalling system also regulates life-history reaction norms by exposing the zooplankton Daphnia magna to both dopamine and the dopamine reuptake inhibitor bupropion, an antidepressant that enters aquatic environments via various pathways. We recorded a range of life-history traits across two food levels. Both treatments induced changes to the life-history reaction norm slopes. These were due to the effects of the treatments being more pronounced at restricted food ration, where controls had lower somatic growth rates, higher age and larger size at maturation. This translated into a higher population growth rate ( r ) of dopamine and bupropion treatments when food was restricted. Our findings show that the dopamine system is an important regulatory mechanism underlying life-history trait responses to food abundance and that bupropion can strongly influence the life history of aquatic species such as D. magna . We discuss why D. magna do not evolve towards higher endogenous dopamine levels despite the apparent fitness benefits.

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Section: Introductionmentioning

confidence: 99%

Dopamine mediates life-history responses to food abundance in Daphnia

et al. 2020

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“…There is an increasing number of studies that have used video tracking devices to assess changes in Daphnia swimming behaviour upon exposure to chemicals (Artells et al, 2013;Bahrndorff et al, 2016;Barrozo et al, 2015;Bownik et al, 2018;Cano et al, 2017;Cruzeiro et al, 2017;Chevalier et al, 2015;Ferrario et al, 2018;Häder and Erzinger, 2017;Hansen and Roslev, 2016;Huang et al, 2017;Huang et al, 2015;Liu et al, 2018;Madeira et al, 2018;Nielsen and Roslev, 2018;Nikitin et al, 2018;Noss et al, 2013;Parolini et al, 2018;Ren et al, 2017;Ren et al, 2015;Stanley et al, 2016;Yang et al, 2018;Zein et al, 2014;Zhang et al, 2016). However, only few of them reported behavioural effects at environmental relevant concentrations far below those causing any sublethal effects on stress markers or lifehistory traits (Nielsen and Roslev, 2018).…”

Section: Discussionmentioning

confidence: 99%

Using a new high-throughput video-tracking platform to assess behavioural changes in Daphnia magna exposed to neuro-active drugs

Simão

Martínez‐Jerónimo

Blasco

et al. 2019

Science of The Total Environment

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One of the major challenges that faces today regulatory risk assessment is to speed up the way of assessing threshold sublethal detrimental effects of existing and new chemical products. Recently advances in imaging allows to monitor in real time the behaviour of individuals under a given stress. Light is a common stress for many different organisms. Fish larvae and many invertebrate species respond to light altering their behaviour. The water flea Daphnia magna as many other zooplanktonic species has a marked diel vertical phototactic swimming behaviour against light due to fish predation. The aim of this study was to develop a highthroughput image analysis to study changes in the vertical swimming behaviour to light of D. magna first reproductive adult females exposed to 0.1 and 1 µg/L of four psychiatric drugs: diazepam, fluoxetine, propranolol and carbamazepine during their entire life. Experiments were conducted using a new custom designed vertical oriented four 50 mL chamber device controlled by the Noldus software (Netherlands). Changes in speed, preferred area (bottom vs upper areas) and animal aggregation were analysed using groups of animals under consecutive periods of dark and apical light stimulus of different intensities. Obtained results indicated that light intensity increased the speed but low light intensities allowed to better discriminate individual responses to the studied drugs. The four tested drugs decreased the response of exposed organisms to light: individuals move less, were closer to the bottom and at low light intensities were closer each other. At high light intensities, however, exposed individuals were less aggregated. Propranolol, carbamazepine and fluoxetine were the compounds effecting most the behaviour. Our results indicated that psychiatric drugs at environmental relevant concentrations alter the vertical phototactic behaviour of D. magna individuals and that it is possible to develop appropriate high-throughput image analysis devices to measure those responses.

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“…DA 1 receptors are involved in coordinating metamorphosis in Drosophila [ 39 ], and DA 1 and DA 2 receptors regulate the phase change of migratory locust in two different directions[ 40 ]. In the inner retina, the DA 2 receptor also plays a role in regulating the development of light responses[ 41 ], and in Daphnia magna , the DA 2 receptor is involved in swimming behavior[ 42 ]. In the mushroom body of the silkworm, DA 2 plays a role in the release of the diapauses hormone[ 29 ].…”

Section: Discussionmentioning

confidence: 99%

Cloning and functional characterization of the DA2 receptor gene in Chinese mitten crab (Eriocheir sinensis)

Yang

Huang

et al. 2018

PLoS ONE

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Dopamine (DA) plays a modulatory role in numerous physiological processes such as light adaptation and food intake, and exerts these functions through DA receptors (DARs). This study presents, for the first time, isolation and characterization of the dopamine receptor 2(DA2 receptor) cDNA from the intestinal tissue of Eriocheir sinensis, an economically important freshwater aquaculture species in China. The DA2 receptor cDNA sequence, which was obtained by rapid amplification of cDNA ends, is 2369bp long, encode peptide of 589 amino acid, and is highly homologous to related sequences in crustaceans. Analysis of the deduced amino acid sequence and the structure of the DA2 indicated that this receptor is a member of the family of G protein-coupled receptors (GPCRs), as it contains seven transmembrane domains and other common signatures of GPCRs. RT-PCR showed that the expression of the DA2 receptor gene was distributed in various tissues, and high expression levels were observed in the cranial ganglia and the thoracic ganglia. Further study of the effect of photoperiod on DA2 expression showed that constant darkness induced a significant increase in DA2 expression in the cranial ganglia. Finally, analysis of DA2 receptor expression under different feeding statuses showed that there was significantly greater expression in the hepatopancreas and intestines after feeding than before feeding, but there were no differences in expression between the before feeding and during feeding periods in either tissue. Our results indicate that the DA2 receptor structurally belongs to the family of G protein-coupled receptors, and that the cranial ganglia are the main tissues in which the DA2 receptor participates in light adaptation during dark hours. In addition, the DA2 receptor in E. sinensis may be involved in the physiological regulation of the hepatopancreas and digestive tract after the ingestion of food. This study provides a foundation for further exploration of the light adaptation and digestive functions of the DA2 receptor in decapods.

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Exposure to D2-like dopamine receptor agonists inhibits swimming in Daphnia magna

Cited by 19 publications

References 37 publications

Dopamine mediates life-history responses to food abundance in Daphnia

Dopamine mediates life-history responses to food abundance in Daphnia

Using a new high-throughput video-tracking platform to assess behavioural changes in Daphnia magna exposed to neuro-active drugs

Cloning and functional characterization of the DA2 receptor gene in Chinese mitten crab (Eriocheir sinensis)

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