Exposure to cis- and trans-regioisomers of S-(1,2-dichlorovinyl)-L-cysteine and S-(1,2-dichlorovinyl)-glutathione result in quantitatively and qualitatively different cellular effects in RPTEC/TERT1 cells

Capinha, Liliana; Jennings, Paul; Commandeur, Jan N. M.

doi:10.1016/j.toxlet.2023.06.005

Cited by 1 publication

(1 citation statement)

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“…RPTEC/TERT1 cells [ 20 ] were obtained from Evercyte GmbH (Vienna, Austria). The cells were cultured in a 1:1 mixture of DMEM and Ham’s F-12 nutrient mix supplemented with 2 mM glutamax, 5 µg/mL insulin, 5 µg/mL transferrin, 5 ng/mL sodium selenite, 10 ng/mL EGF, 36 ng/mL hydrocortisone, 100 U/mL penicillin and 100 µg/mL streptomycin (Sigma-Aldrich, P4333), and 0.5% fetal bovine serum (FBS) (Gibco, 10270-106), at 36.5 °C in a 5% CO 2 -humidified environment, as previously described [ 21 ]. For experiments, cells were plated out into the desired plate formats and cultured for a minimum of 10 days after reaching confluency to allow for maturation.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Organic Anion and Cation Transport in Three Human Renal Proximal Tubular Epithelial Models

Meijer,

da Costa Pereira,

Klatt

et al. 2024

Cells

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The polarised expression of specific transporters in proximal tubular epithelial cells is important for the renal clearance of many endogenous and exogenous compounds. Thus, ideally, the in vitro tools utilised for predictions would have a similar expression of apical and basolateral xenobiotic transporters as in vivo. Here, we assessed the functionality of organic cation and anion transporters in proximal tubular-like cells (PTL) differentiated from human induced pluripotent stem cells (iPSC), primary human proximal tubular epithelial cells (PTEC), and telomerase-immortalised human renal proximal tubular epithelial cells (RPTEC/TERT1). Organic cation and anion transport were studied using the fluorescent substrates 4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP) and 6-carboxyfluorescein (6-CF), respectively. The level and rate of intracellular ASP accumulation in PTL following basolateral application were slightly lower but within a 3-fold range compared to primary PTEC and RPTEC/TERT1 cells. The basolateral uptake of ASP and its subsequent apical efflux could be inhibited by basolateral exposure to quinidine in all models. Of the three models, only PTL showed a modest preferential basolateral-to-apical 6-CF transfer. These results show that organic cation transport could be demonstrated in all three models, but more research is needed to improve and optimise organic anion transporter expression and functionality.

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Section: Methodsmentioning

confidence: 99%