Exposure to chronic stressor upsurges the excitability of serotoninergic neurons and diminishes concentrations of circulating corticosteroids in rats two weeks thereafter

Effects of acute and chronic administration of trace amine-associated receptor 1 (TAAR1) ligands on in vivo excitability of central monoamine-secreting neurons in rats

“…The excitability characteristics of catecholamine neurons in control animals were similar to those observed in our previous studies (Table 1) [13,15,16]. Even though the firing activity of 5-HT neurons of the DRN was higher than that observed in Sprague-Dawley rats [13], it was in the similar range as measured in our previous experiments in Wistar rats [14][15][16][17].…”

“…In vivo electrophysiological experiments were performed as previously described [13][14][15][16][17][18][19]. Animals were anesthetized by chloral hydrate (400 mg/ kg, i.p.)…”

“…After completion of electrophysiological recordings, the animals were euthanized by overdose of chloral hydrate. In selected animals, the electrode tip location was labeled by electrolytic lesion using a direct current (DC) of 0.5 mA for 15 s, as previously described [16,17]. The brains were removed and fixed in 10% paraformaldehyde for 24 h, and afterward in 30% sucrose for 7 days.…”

Maternal treatment with a selective delta-opioid receptor agonist during gestation has a sex-specific pro-cognitive action in offspring: mechanisms involved

“…In vivo electrophysiological experiments described previously ( Dremencov et al, 2022 ; Grinchii et al, 2022 ; Dremencov et al, 2023 ; Grinchii et al, 2023 ) were performed in male and female adult offspring of the SNC80- and vehicle-treated dams 24 h following behavioral testing. Urethane (1.25 g/kg, intraperitoneally: i. p.) or chloral hydrate (0.4 g/kg, i. p) anesthesia was used in experiments aiming to assess the excitability of hippocampal glutamate and brainstem monoamine neurons, respectively.…”

“…The neuronal firing rate and burst activity characteristics were calculated using the burstiDAtor software ( www.github.com/nno/burstidator ). The onset of the burst mode of firing and its termination were detected as previously described ( Hajós et al, 2007 ; Csatlosova et al, 2021 ; Dremencov et al, 2022 ; Grinchii et al, 2022 ). Statistical assessments were performed using SigmaPlot 12.5 software (Systat Software Inc., Chicago, IL, United States).…”