Abstract:Objectives: Exposure to organophosphorus (OP) pesticides, irreversible inhibitors of acetylcholinesterase (AChE), may result in long-lasting alterations in the functional state of the central nervous system. In earlier studies, we found that a single exposure of the rat to chlorphenvinphos (CVP), an OP pesticide, made the animal hyposensitive to amphetamine (AMPH) three weeks posttreatment. A repeated administration of AMPH is known to result in a progressive increase in the behavioral sensitivity to the psych… Show more
“…The obtained results, however, show clearly that a single CVP administration, even at a relatively low dose, results in an augmented CORT response to AMPH weeks later. This means that the behavioral hyporeactivity to AMPH [22], and the present results as well as the reduced susceptibility to develop behavioral sensitization upon a repeated AMPH treatment of the CVP pretreated rats [23] could not have resulted from a reduced reactivity of the HPA axis. Moreover, it appears that,…”
Section: This Study Was Supported By the Nofer Institute Of Occupationalmentioning
confidence: 83%
“…CVP is a direct AChE inhibitor. As we have reported earlier, a single exposure of the rat to CVP, even at a moderate dose, makes the animal hyporesponsive behaviorally to amphetamine (AMPH) weeks later [22][23]. Needless to say, AMPH is an indirect dopaminergic agonist and one of the most frequently abused psychostimulants.…”
Section: Chemicals Doses and Drug Administration Proceduresmentioning
confidence: 94%
“…AMPH was administered at 0.5 mg/kg b.w. The pesticide and AMPH doses were in the range of doses used in our earlier experiments [23]. In all cases, the injections as well as the collection of blood samples took place outside the animal room.…”
Section: Chemicals Doses and Drug Administration Proceduresmentioning
Objectives: Exposure to various stressors is known to result in sensitization to psychostimulants, a state related to the psychostimulant dependence and addiction. It has been shown in some studies that the rise in corticosterone (CORT) concentration is indispensable for both the induction and the expression of behavioral sensitization. Therefore, it might be suspected that behavioral hyposensitivity to amphetamine (AMPH) is somehow related to a reduced CORT response to the psychostimulant subsequent to the chlorphenvinphos (CVP) intoxication. Materials and Methods: The male adult Wistar rats received single i.p. injections of CVP at the doses 0.5, 1.0 or 3.0 mg/kg b.w., or pure corn oil. CORT concentration was determined in samples of blood drawn from the tail vein before and then 30, 60, 180 min and 24 h after injection. The other rats were divided into two groups and tested, three weeks after the CVP injection for the effect of AMPH (0.5 mg/kg b.w. i.p.) on the serum CORT concentration. In addition, behavioral sensitivity to AMPH was assessed by measuring locomotor activity of the animals in an open-field. Results: 1) The stressor property of CVP was confirmed. The injection resulted in up to tenfold increase in the serum CORT concentration. The magnitude and duration of this response were dose-related. 2) Three weeks after the CVP exposure, the CORT response to AMPH was significantly increased.3) The behavioral response to the psychostimulant, i.e. augmented locomotion, was significantly reduced compared to the control. Conclusions: The results confirm that CVP exposure causes behavioral hyposensitivity to AMPH. This effect, however, could not be ascribed to a diminished CORT response.
“…The obtained results, however, show clearly that a single CVP administration, even at a relatively low dose, results in an augmented CORT response to AMPH weeks later. This means that the behavioral hyporeactivity to AMPH [22], and the present results as well as the reduced susceptibility to develop behavioral sensitization upon a repeated AMPH treatment of the CVP pretreated rats [23] could not have resulted from a reduced reactivity of the HPA axis. Moreover, it appears that,…”
Section: This Study Was Supported By the Nofer Institute Of Occupationalmentioning
confidence: 83%
“…CVP is a direct AChE inhibitor. As we have reported earlier, a single exposure of the rat to CVP, even at a moderate dose, makes the animal hyporesponsive behaviorally to amphetamine (AMPH) weeks later [22][23]. Needless to say, AMPH is an indirect dopaminergic agonist and one of the most frequently abused psychostimulants.…”
Section: Chemicals Doses and Drug Administration Proceduresmentioning
confidence: 94%
“…AMPH was administered at 0.5 mg/kg b.w. The pesticide and AMPH doses were in the range of doses used in our earlier experiments [23]. In all cases, the injections as well as the collection of blood samples took place outside the animal room.…”
Section: Chemicals Doses and Drug Administration Proceduresmentioning
Objectives: Exposure to various stressors is known to result in sensitization to psychostimulants, a state related to the psychostimulant dependence and addiction. It has been shown in some studies that the rise in corticosterone (CORT) concentration is indispensable for both the induction and the expression of behavioral sensitization. Therefore, it might be suspected that behavioral hyposensitivity to amphetamine (AMPH) is somehow related to a reduced CORT response to the psychostimulant subsequent to the chlorphenvinphos (CVP) intoxication. Materials and Methods: The male adult Wistar rats received single i.p. injections of CVP at the doses 0.5, 1.0 or 3.0 mg/kg b.w., or pure corn oil. CORT concentration was determined in samples of blood drawn from the tail vein before and then 30, 60, 180 min and 24 h after injection. The other rats were divided into two groups and tested, three weeks after the CVP injection for the effect of AMPH (0.5 mg/kg b.w. i.p.) on the serum CORT concentration. In addition, behavioral sensitivity to AMPH was assessed by measuring locomotor activity of the animals in an open-field. Results: 1) The stressor property of CVP was confirmed. The injection resulted in up to tenfold increase in the serum CORT concentration. The magnitude and duration of this response were dose-related. 2) Three weeks after the CVP exposure, the CORT response to AMPH was significantly increased.3) The behavioral response to the psychostimulant, i.e. augmented locomotion, was significantly reduced compared to the control. Conclusions: The results confirm that CVP exposure causes behavioral hyposensitivity to AMPH. This effect, however, could not be ascribed to a diminished CORT response.
“…Weeks after an intermittent or even single administration of AMPH, the behavioural response to AMPH challenge is augmented [29]. Exposure to an organophosphorus pesticide, on the other hand, results, weeks later, in behavioural hyposensitivity to an AMPH challenge [24,30]. In both cases, however, the humoral response to the AMPH challenge, i.e.…”
Section: Figmentioning
confidence: 99%
“…Each cage (63×63×40 cm) was equipped with 2 tiers of infrared motion sensors allowing to measure locomotor (travelled distance) and exploratory (rearing) activities. Detailed description of the apparatus have been presented in other reports from this laboratory [24].…”
Objectives: Some data suggest that increased behavioural sensitivity to psychostimulants may develop after exposure to volatile chemicals in common use. The purpose of the present experiment was to find out whether and in what way inhalation exposure to pseudocumene (PS) or hemimellitene (HM) at low concentrations alters behavioural sensitivity to the psychostimulant amphetamine (AMPH), and propensity to develop behavioural sensitization to AMPH. Material and Methods: Adult male Wistar rats were exposed 6 h/day, 5 days a week for 4 weeks to PS or HEM at 0, 25, 100 or 250 ppm. Behavioural sensitivity to AMPH was assessed by measuring locomotor activity of the animals in an open-field. Behavioural sensitization to AMPH was induced by a repeated AMPH treatment. Results: In rats exposed to HEM, the behavioural sensitivity to AMPH was increased, but remained unchanged in rats exposed to PS. The second testing revealed an augmented behavioural response to AMPH in control rats. In the HM exposed rats this augmenting was significantly more evident and in the PS exposed rats significantly less evident than in controls. For each of the two solvents, the concentration-effect relationship was nonlinear; out of the three concentrations used, 100 ppm was the most effective. Conclusions: The results confirm that low-level inhalation exposure to trimethylbenzene isomers may induce behavioural sensitisation and/or increase the susceptibility of the animals to develop this state upon repeated psychostimulant treatment. They show, however, that HM and PS differ markedly in their ability to induce such alterations.
Malathion is a highly neurotoxic pesticide widely used in daily life. Acute and chronic toxicity from this organophosphorus compound may cause damage to health, especially to the central nervous system. In the present work, we show the effects of chronic exposure of malathion on dendritic morphology of neurons from prefrontal cortex (PFC), hippocampus, and nucleus accumbens (NAcc) in adult male mice. Animals were injected i.p. with low dose of malathion (40 mg/kg body weight) for 14 days. Control animals were injected with corn oil, used as vehicle. Fourteen days after the last injection, brains were removed and processed by the Golgi-Cox stain method, and coronal sections were obtained to perform Sholl analysis on pyramidal neurons from the PFC, CA1 area from the hippocampus, and medium spiny cells from the NAcc. Dendritic morphology analysis included the total dendritic length, the maximum branching order, and the dendritic spine density. Results indicated a significant decrement on dendritic morphology in neurons from the hippocampus and the PFC in animals injected with malathion, whereas medium spiny neurons from NAcc showed a significant decrement only on the dendritic spine density in malathion injected mice, as compared to control mice. These results suggest that chronic toxicity of malathion alters the dendritic morphology in adult age, which may affect behavior.
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