Exposure to carbon dioxide and helium reduces in vitro proliferation of pediatric tumor cells

Schmidt, A; Reismann, Marc; Kübler, J.; Vieten, Gertrud; Bangen, Cathérine; Shimotakahara, Akihiro; Glüer, S.; Nustede, R.; Ure, Benno M.

doi:10.1007/s00383-005-1585-4

Cited by 18 publications

(11 citation statements)

References 28 publications

(31 reference statements)

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“…In addition, it has been shown that CO 2 alters the functions of macrophages and polymorphonuclear cells in the peritoneal and thoracic cavity [19,32,35,36,41]. We have confirmed a downregulation of the release of various cytokines, free oxygen radicals, nitric oxide, and of the mitochondrial activity of macrophages and polymorphonuclear cells during and after CO 2 exposition via alteration of the extracellular pH [23,25].…”

Section: Discussionsupporting

confidence: 70%

“…Some authors reported an increase in cell proliferation and tumor growth [13,18,20,30], whereas others found beneficial effects of CO 2 exposition [1,27,37]. We showed previously [35] that exposure to CO 2 decreased the mitochondrial activity of several neuroblastoma cell lines, and of lymphoma and hepatocellular carcinoma cells in vitro. This phenomenon persisted over 4 days.…”

Section: Discussionmentioning

confidence: 73%

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CO2 pneumoperitoneum increases systemic but not local tumor spread after intraperitoneal murine neuroblastoma spillage in mice

et al. 2008

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Section: Discussionsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 73%

CO2 pneumoperitoneum increases systemic but not local tumor spread after intraperitoneal murine neuroblastoma spillage in mice

et al. 2008

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“…Takiguchi et al [20] reported that the number of WiDr cells under pneumoperitoneal conditions decreased in the first 24 h when WiDr human colon cancer cells were incubated for 3 h at 100% CO 2 at 10 mmHg. Schmidt et al [21] reported that the proliferation of neuroblastoma, lymphoma, hepatoblastoma and hepatocellular carcinoma cells decreased significantly following 2 h in a CO 2 environment. This decrease lasted more than four days in neuroblastoma, lymphoma and hepatocellular carcinoma cells.…”

Section: Discussionmentioning

confidence: 98%

Effects of HIF-1α on human gastric cancer cell apoptosis at different CO2 pressures

Hao

Zhang

et al. 2008

Clin Exp Med

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The effects and potential molecular mechanisms underlying carbon dioxide (CO(2)) pneumoperitoneum on gastric cancer cell apoptosis are not fully understood. In this study, we assessed the effects of CO(2) pneumoperitoneum on the apoptosis of MKN-45 gastric cancer cells. Additionally, we investigated the role of HIF-1alpha in CO(2) pneumoperitoneum-induced apoptosis of gastric cancer cells. MKN-45 cells were cultured in CO(2) or air pneumoperitoneum at 0, 12 and 15 mmHg pressures for 4 h. We observed a change in cells morphology and increasing apoptotic ratios in MKN-45 cells when they were put into a 15 mmHg CO(2) pneumoperitoneum environment. However, there was no significant difference between the 0, 12 mmHg CO(2) pneumoperitoneum and the control groups. Exposure to 15 mmHg CO(2) pneumoperitoneum significantly enhanced the expression levels of HIF-1alpha and Bax, while it attenuated Bcl-2 expression levels. When we inhibited HIF-1alpha by small interfering RNA (siRNA), we found that the apoptotic ratio of MKN-45 cells decreased in 15 mmHg CO(2) pneumoperitoneum. This treatment markedly elevated Bcl-2 levels and decreased Bax expression. These data suggest that CO(2) pneumoperitoneum may accelerate the apoptosis of MKN-45 cells at higher pressures. HIF-1alpha is a crucial factor that affects gastric cancer cell apoptosis by downregulating the Bcl-2/Bax ratio.

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“…Schmidt et al 28 found a significant reduction of the proliferation rate of neuroblastoma, hepatoblastoma, hepatocellular carcinoma, and lymphoma cells for up to 4 days after exposure to carbon dioxide vs air and helium in vitro. Experimental studies 29 have not yet been able to confirm an alteration in the behavior of pediatric tumor cells by MIS.…”

Section: Discussionmentioning

confidence: 99%

Role of diagnostic and ablative minimally invasive surgery for pediatric malignancies

Metzelder

Kuebler

Shimotakahara

et al. 2007

Cancer

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BACKGROUNDThe use of minimally invasive surgery (MIS) in pediatric cancer is a matter of debate. The diagnostic and ablative roles of MIS were evaluated in a consecutive series of children with malignancies.METHODSA prospective study, including all patients, who underwent abdominal and thoracic surgery for confirmed or highly suspected pediatric cancer was performed from September, 2000, to December, 2005. An interdisciplinary panel approved the indication for minimally invasive or conventional surgery.RESULTSAt a single institution, 301 operations were performed on 276 children with cancer. A minimally invasive approach was attempted in 90 of these patients (30%) and successfully employed in 69 (77%) of the operations. However, 21 operations (23%) were converted to an open approach. Regarding the abdominal operations attempted laparoscopically, 41 abdominal operations for biopsy or staging purposes were attempted laparoscopically (53%), but 6 were converted. In all, 139 abdominal resections were performed and 24 were attempted laparoscopically. Ten of these (42%) were converted. In 34 thoracic operations requiring biopsy, thoracoscopy was attempted in 14 (41%) and was successful in all but 1 (93%). Fifty‐one thoracic tumors were resected and the thoracoscopic approach was attempted in 11 (22%) and successful in 7 (14%). Conversions from a minimally invasive operation to an open procedure occurred mainly due to limited visibility. Three bleeding complications occurred with 1 patient requiring a blood transfusion. In addition, 1 small bowel injury occurred with immediate laparoscopic closure. There were no port site recurrences after a median of 39 months.CONCLUSIONSMIS is a reliable diagnostic tool for pediatric abdominal and thoracic malignancy. The role of ablative MIS in pediatric cancer remains limited. Cancer 2007. © 2007 American Cancer Society.

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Exposure to carbon dioxide and helium reduces in vitro proliferation of pediatric tumor cells

Cited by 18 publications

References 28 publications

CO2 pneumoperitoneum increases systemic but not local tumor spread after intraperitoneal murine neuroblastoma spillage in mice

CO2 pneumoperitoneum increases systemic but not local tumor spread after intraperitoneal murine neuroblastoma spillage in mice

Effects of HIF-1α on human gastric cancer cell apoptosis at different CO2 pressures

Role of diagnostic and ablative minimally invasive surgery for pediatric malignancies

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