Exposure to benzo(a)pyrene from juvenile period to peripubertal impairs male reproductive parameters in adult rats

Jorge, Bárbara Campos; Reis, Ana Carolina Casali; Sterde, Érika Tissiana; Balin, Paola da Silva; Scarano, Wellerson Rodrigo; Hisano, Hamilton; Arena, Arielle Cristina

doi:10.1016/j.chemosphere.2020.128016

Cited by 22 publications

(8 citation statements)

References 57 publications

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“…The oral exposure to BaP resulted in developmental, immunological, and reproductive effects such as decreased fertility and neurobehavioral alterations in animal models [ 8 , 17 , 19 , 23 , 24 , 25 , 26 , 27 ]. Reproductive defects included the impairment of ovary weight, sperm counts and follicles numbers [ 8 , 15 , 16 , 17 , 19 , 25 , 27 ]. Moreover, epidemiological studies in humans reported adverse birth outcomes, including reduced birth and postnatal body weight and lower head circumference, other developmental, neurobehavioral effects that are generally analogous to those observed in animals, providing supportive evidence for hazards associated with BaP exposure [ 10 , 23 , 28 , 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

“…In mouse models, the exposure to BaP results in reproductive dysfunction, such as reduced production of spermatozoa, lower serum testosterone levels and impairment of oocyte meiotic progression resulting in a low fertilization rate [ 8 , 15 , 16 ]. In addition, exposure of BaP causes reproductive damages to aquatic organisms, affecting sexual differentiation and hatching time in medaka fish and impacting ovarian development by disrupting oocytes in Chlamys farreri [ 17 , 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Short-Term Exposure to Benzo(a)Pyrene Causes Disruption of GnRH Network in Zebrafish Embryos

Gentile

Vezzoli

Martone

et al. 2023

IJMS

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Benzo(a)pyrene (BaP), a polycyclic aromatic hydrocarbon, is considered a common endocrine disrupting chemical (EDC) with mutagenic and carcinogenic effects. In this work, we evaluated the effects of BaP on the hypothalamo-pituitary-gonadal axis (HPG) of zebrafish embryos. The embryos were treated with 5 and 50 nM BaP from 2.5 to 72 hours post-fertilization (hpf) and obtained data were compared with those from controls. We followed the entire development of gonadotropin releasing hormone (GnRH3) neurons that start to proliferate from the olfactory region at 36 hpf, migrate at 48 hpf and then reach the pre-optic area and the hypothalamus at 72 hpf. Interestingly, we observed a compromised neuronal architecture of the GnRH3 network after the administration of 5 and 50 nM BaP. Given the toxicity of this compound, we evaluated the expression of genes involved in antioxidant activity, oxidative DNA damage and apoptosis and we found an upregulation of these pathways. Consequently, we performed a TUNEL assay and we confirmed an increment of cell death in brain of embryos treated with BaP. In conclusion our data reveal that short-term exposure of zebrafish embryos to BaP affects GnRH3 development likely through a neurotoxic mechanism.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Short-Term Exposure to Benzo(a)Pyrene Causes Disruption of GnRH Network in Zebrafish Embryos

Gentile

Vezzoli

Martone

et al. 2023

IJMS

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“…The females were orally (gavage) treated with three different doses of benzo(a)pyrene (BaP) (96% purity, Sigma–Aldrich Co. Ltd.) diluted in corn oil +0.5% of dimethylsulfoxide (vehicle) (0.1; 1.0 or 10 μg/kg), while one group received 1 ml/kg of vehicle and was used as control. The BaP doses were based on studies performed by Reddy et al 18 and our research group 19 . Our lowest dose was chosen because it is less than the candidate value for no‐observed‐adverse‐effect level (NOAEL) of BaP for reproductive effects in rats (0.2 μg/kg) 20 .…”

Section: Methodsmentioning

confidence: 99%

Effects of benzo(a)pyrene at environmentally relevant doses on embryo‐fetal development in rats

Moreira

Inocêncio

Jorge

et al. 2020

Environmental Toxicology

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Studies have demonstrated that Benzo(a)Pyrene (BaP), a polycyclic aromatic hydrocarbon ubiquituous in the environment, can cause teratogenic effects. Since the majority of studies used in vitro models or high doses of BaP, this study evaluated the teratogenicity, reproductive and developmental performance of low doses of BaP through maternal and fetus examination after daily oral administration of BaP (0; 0.1; 1.0 or 10 μg/kg) to pregnant Wistar rats from Gestational day (GD) 6 to GD 15 (the organogenesis period). Pregnant rats did not exhibit clinical signs of toxicity during the exposure period. However, dams exposed to the lowest dose of BaP showed a reduction in the erythrocytes number and in the creatinine levels. The groups exposed to 0.1 and 1.0 μg/kg presented a decrease in placental efficiency, as well as an increase in placental weight. After fetal examination, the treated group with the lowest dose showed a reduced relative anogenital distance, while the curve of normal distribution of weight was changed in the highest dose group. In addition, anomalies evidenced by changes in the renal size and degree of fetal ossification were observed in treated‐fetus. In conclusion, treatment with BaP during organogenesis at this dose level is detrimental to the normal development of fetuses.

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“…Benzo(a)pyrene alters sperm functional competence, evidenced by a reduced percentage of acrosome halo formation and sperm hyperactivation [154]. Prepubertal exposure to Benzo(a)pyrene alters the male reproductive parameters [155]. Even though the relationship between tobacco smoking and semen quality has remained controversial for the past several decades, most studies have reported significant changes in the conventional semen parameters, including semen volume, sperm density, motility, viability, and normal morphology in the smoking population and suggesting that smoking harms the male reproductive health [155,156].…”

Section: Cigarette Smoke and Endocrine Disruptorsmentioning

confidence: 99%

Recent Updates on the Effect of Endocrine Disruptors on Male Reproductive Functions

Rajendran¹,

Latchoumycandane²,

Mathur³

2022

MEDJ

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Endocrine disruptors are man-made or naturally occurring chemical substances, upon exposure, alter the male reproductive health by interfering with hormonal homeostasis and spermatogenesis. Several studies have supported the hypothesis that a decrease in sperm count over the past few decades is due to exposure to environmental contaminants possessing estrogenic or anti-androgenic properties. Bisphenol A, phthalates, alkylphenols, and polychlorinated biphenyls are some of the endocrine-disrupting chemicals commonly present in our day-to-day products that have been shown to pose a significant threat to reproductive health. Many chemicals directly or indirectly affect the endocrine systems, altering metabolism, sex differentiation, growth, stress response, gender behavior, and reproduction. The endocrine pathway disruption is possible via membrane receptors or nuclear receptors and inhibition of enzymatic pathways. The declining male reproductive health has been linked to an increased presence of chemical contaminants in our environment in the form of pesticides and plastics. The effect of endocrine disruptors on reproductive health remains a real issue considering public health. This review gives a recent update on environmental chemicals that have endocrine-disrupting potential and their effect on the male reproductive system.

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Exposure to benzo(a)pyrene from juvenile period to peripubertal impairs male reproductive parameters in adult rats

Cited by 22 publications

References 57 publications

Short-Term Exposure to Benzo(a)Pyrene Causes Disruption of GnRH Network in Zebrafish Embryos

Short-Term Exposure to Benzo(a)Pyrene Causes Disruption of GnRH Network in Zebrafish Embryos

Effects of benzo(a)pyrene at environmentally relevant doses on embryo‐fetal development in rats

Recent Updates on the Effect of Endocrine Disruptors on Male Reproductive Functions

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