Exposure to alcohol or tobacco affects the pattern of maturation in oral mucosal cells: a cytohistological study

Burzlaff, João Batista; Bohrer, Paula Luce; Paiva, R L; Visioli, Fernanda; Filho, Manoel Sant’Ana; Silva, V D da; Rados, Pantelis Varvaki

doi:10.1111/j.1365-2303.2007.00473.x

Cited by 24 publications

(37 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…2 Noninvasive methods, such as cytopathology, have proved effective in the early detection of cell changes that are not clinically visible in patients exposed to risk factors and in potentially malignant lesions. 3,4,5,6 However, to have a better understanding of the risk of malignancy of each lesion, the correlation of cytopathology with complementary techniques such as immunocytochemistry may improve the effectiveness of this screening process. The expression of markers associated with cell adhesion (e.g., E-cadherin) and with cell differentiation (e.g., involucrin) may help predict the risk of malignancy, as their functions are dysregulated during carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

Expression of E-cadherin and involucrin in leukoplakia and oral cancer: an immunocytochemical and immunohistochemical study

et al. 2017

View full text Add to dashboard Cite

To assess the immunocytochemical and immunohistochemical correlation of adhesion (E-cadherin) and cell differentiation (involucrin) molecules in oral leukoplakia and oral squamous cell carcinoma. Cytological samples and biopsies were obtained from male and female patients aged over 30 years with oral leukoplakia (n = 30) and oral squamous cell carcinoma (n = 22). Cell scrapings and the biopsy were performed at the site of the lesion and histological slides were prepared for the immunocytochemical analysis of exfoliated oral mucosal cells and for the immunohistochemical analysis of biopsy tissues using E-cadherin and involucrin. Spearman's correlation and kappa coefficients were used to assess the correlation and level of agreement between the techniques. Immunostaining for E-cadherin and involucrin by both techniques was similar in the superficial layers of the histological sections compared with cell scrapings. However, there was no statistical correlation and agreement regarding the immunocytochemical and immunohistochemical expression of E-cadherin and involucrin in oral leukoplakia (R = 0.01, p = 0.958) (Kappa = 0.017, p = 0.92) or in oral squamous cell carcinoma (R = 0.26, p = 0.206) (Kappa = 0.36, p = 0.07). The immunoexpression of E-cadherin and involucrin in tissues is consistent with the expression patterns observed in exfoliated oral mucosal cells, despite the lack of a statistically significant correlation. There is an association of the histopathological characteristics of leukoplakia with the expression E-cadherin and of the microscopic aspects of oral squamous cell carcinoma with immunohistochemical expression of involucrin.

show abstract

Section: Introductionmentioning

confidence: 99%

Expression of E-cadherin and involucrin in leukoplakia and oral cancer: an immunocytochemical and immunohistochemical study

et al. 2017

View full text Add to dashboard Cite

show abstract

“…With the introduction of quantitative techniques, oral cytology has become an important preventive exam for monitoring the oral mucosa exposed to carcinogens [2][3][4][5][6] . From these studies, it became apparent the individual nature of this type of evaluation, suggesting that some individuals are more susceptible than others to oral cancer development.…”

Section: Introductionmentioning

confidence: 99%

“…Over time, many studies have sought to evaluate the influence of extrinsic factors (tobacco and alcohol consumption) and intrinsic factors such as sex and age in the process of oral epithelium maturation 2,7 . However, it remains uncertain whether the consumption of alcohol and tobacco, in addition to individual parameters modify the normal process of epithelial maturation of the oral cavity.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Influence of factors in the oral mucosa maturation pattern: a cross-sectional study applying multivariate analyses

Baumgart¹,

Daroit²,

Maraschin³

et al. 2016

Braz. J. Oral Sci.

View full text Add to dashboard Cite

Aim: To evaluate the association between oral health status, socio-demographic and behavioral factors with the pattern of maturity of normal epithelial oral mucosa. Methods: Exfoliative cytology specimens were collected from 117 men from the border of the tongue and floor of the mouth on opposite sides. Cells were stained with the Papanicolaou method and classified into: anucleated, superficial cells with nuclei, intermediate and parabasal cells. Quantification was made by selecting the first 100 cells in each glass slide. Sociodemographic and behavioral variables were collected from a structured questionnaire. Oral health was analyzed by clinical examination, recording decayed, missing and filled teeth index (DMFT) and use of prostheses. Multivariable linear regression models were applied. Results: No significant differences for all studied variables influenced the pattern of maturation of the oral mucosa except for alcohol consumption. There was an increase of cell surface layers of the epithelium with the chronic use of alcohol. Conclusions: It is appropriate to use Papanicolaou cytopathological technique to analyze the maturation pattern of exposed subjects, with a strong recommendation for those who use alcohol -a risk factor for oral cancer, in which a change in the proportion of cell types is easily detected.

show abstract

“…D' ailleurs, l' inconfort voire l' intolérance au port prothétique est fréquent(e), à cause de la santé tissulaire défaillante, de la xérostomie, de l' hygiène (orale et prothétique) aléatoire, d' une éventuelle candidose, d'autant plus que le matériel prothétique a subi un vieillissement prématuré par action de l' éthanol sur les propriétés physiques de la résine acrylique [51]. S' agissant d' anomalies muqueuses, bien que controversé en tant qu' étiologie à part entière, car souvent combiné au tabac, l' alcool ferait le lit des lésions précancéreuses [52][53][54][55] telles que les leucoplasies homogènes ou inhomogènes [56,57]. La leucoplasie est une lésion blanche kératosique de la muqueuse buccale qui ne peut être détachée par le grattage.…”

Section: Effets De L' Alcool Sur Les Tissus Mousunclassified

L’alcool : une drogue licite aux conséquences bucco-dentaires non négligeables

Pesci-Bardon¹,

Prêcheur²

2013

Actual. Odonto-Stomatol.

View full text Add to dashboard Cite

show abstract

Exposure to alcohol or tobacco affects the pattern of maturation in oral mucosal cells: a cytohistological study

Abstract: The maturation pattern of cells in the three anatomic sites showed changes that may be associated with the synergistic effect of tobacco and alcohol. Also, the severity of histopathological findings was associated with the increase in the number of cells in the deeper epithelial layers.

Cited by 24 publications

References 22 publications

Expression of E-cadherin and involucrin in leukoplakia and oral cancer: an immunocytochemical and immunohistochemical study

Expression of E-cadherin and involucrin in leukoplakia and oral cancer: an immunocytochemical and immunohistochemical study

Influence of factors in the oral mucosa maturation pattern: a cross-sectional study applying multivariate analyses

L’alcool : une drogue licite aux conséquences bucco-dentaires non négligeables

Contact Info

Product

Resources

About