Exposure to Al2O3 nanoparticles facilitates conjugative transfer of antibiotic resistance genes from Escherichia coli to Streptomyces

Liu, Xiaomei; Tang, Jingchun; Song, Benru; Zhen, Mengmeng; Wang, Lan; Giesy, John P.

doi:10.1080/17435390.2019.1669731

Cited by 31 publications

(25 citation statements)

References 41 publications

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“…Different studies have shown that sublethal concentrations of ROS-generating antibiotics and biocides promote conjugation via the SOS-response [81,92,93]. Accordingly, the conjugative transfer was found to decrease in the presence of ROS scavengers, supporting the role of ROS in this process [81,89]. Moreover, ROS generation in mating bacteria has been observed with ROS-specific fluorescent dyes, RNAseq, and proteome analysis.…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 81%

“…Importantly, field studies have described that long-term metal contamination co-selects for antibiotic-resistant genes (ARGs) and metal-resistant genes in different environments such as wastewater treatment plants [21,76], farms [77], and agriculture soils [78,79]. Furthermore, experimental evidence gathered over the last few years suggests that metallic NPs might facilitate the spread of antibiotic resistance between bacteria through co-selection and horizontal gene transference (HGT) of ARGs as well [62,[80][81][82]. Hence, given the increasing use of NPs, concern is increasing as prolonged exposure of bacteria to sublethal levels of NPs could act as selective pressure that may accelerate the spread of antibiotic resistance in the environment.…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

“…It has been proposed that ROS generated by NPs would lead to both membrane and DNA damage. Indeed, membrane damage and an increased number of conjugative junctions have been observed by transmission electron microscopy (TEM) in E. coli and P. putida cells exposed to AgO-NPs [81], Al2O3-NPs [80], or CuO-NPs [89]. On one hand, membrane damage could favor DNA uptake by impairing the membrane barrier.…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

“…On one hand, membrane damage could favor DNA uptake by impairing the membrane barrier. This has been evidenced by different experimental approaches such as flow cytometry, RNAseq, and proteome analysis of conjugating bacteria exposed to CuO-NPs [89] or Ag-NPs [81]. On the other hand, damaged DNA would trigger the SOS response, which in turn might promote HGT by different mechanisms [90].…”

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

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Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

et al. 2021

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The rapid spread of antibiotic resistances among bacteria demands novel strategies for infection control, and metallic nanoparticles appear as promising tools because of their unique size and tunable properties that allow their antibacterial effects to be maximized. Furthermore, their diverse mechanisms of action towards multiple cell components have suggested that bacteria could not easily develop resistance against nanoparticles. However, research published over the last decade has proven that bacteria can indeed evolve stable resistance mechanisms upon continuous exposure to metallic nanoparticles. In this review, we summarize the currently known individual and collective strategies employed by bacteria to cope with metallic nanoparticles. Importantly, we also discuss the adverse side effects that bacterial exposure to nanoparticles may have on antibiotic resistance dissemination and that might constitute a challenge for the implementation of nanoparticles as antibacterial agents. Overall, studies discussed in this review point out that careful management of these very promising antimicrobials is necessary to preserve their efficacy for infection control.

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Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 81%

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

Section: Arming the Enemy: Metallic Nps May Act As Pressure That Co-smentioning

confidence: 99%

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Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

et al. 2021

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“…Khan et al [29] examined the three-dimensional unsteady CNTs nanofluid flow in the existence of velocity and thermal slip. Recently many researchers investigated the influence of different nanoparticle in various flow fields [30][31][32][33][34][35].…”

Section: Introductionmentioning

confidence: 99%

On the Cattaneo–Christov Heat Flux Model and OHAM Analysis for Three Different Types of Nanofluids

et al. 2020

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In this article, the boundary layer flow of a viscous nanofluid induced by an exponentially stretching surface embedded in a permeable medium with the Cattaneo–Christov heat flux model (CCHFM) is scrutinized. We took three distinct kinds of nanoparticles, such as alumina (Al2O3), titania (TiO2) and copper (Cu) with pure water as the base fluid. The features of the heat transfer mechanism, as well as the influence of the relaxation parameter on the present viscous nanofluid flow are discussed here thoroughly. The thermal stratification is taken in this phenomenon. First of all, the problem is simplified mathematically by utilizing feasible similarity transformations and then solved analytically through the OHAM (optimal homotopy analysis method) to get accurate analytical solutions. The change in temperature distribution and axial velocity for the selected values of the specific parameters has been graphically portrayed in figures. An important fact is observed when the thermal relaxation parameter (TRP) is increased progressively. Graphically, it is found that an intensification in this parameter results in the exhaustion of the fluid temperature together with an enhancement in the heat transfer rate. A comparative discussion is also done over the Fourier’s law and Cattaneo–Christov model of heat.

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Characterization and functional evidence for Orf2 of Streptomyces sp. 139 as a novel dipeptidase E

Liu,

Li,

et al. 2024

Appl Microbiol Biotechnol

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Aspartyl dipeptidase (dipeptidase E) can hydrolyze Asp-X dipeptides (where X is any amino acid), and the enzyme plays a key role in the degradation of peptides as nutrient sources. Dipeptidase E remains uncharacterized in Streptomyces. Orf2 from Streptomyces sp. 139 is located in the exopolysaccharide biosynthesis gene cluster, which may be a novel dipeptidase E with “S134-H170-D198” catalytic triad by sequence and structure comparison. Herein, recombinant Orf2 was expressed in E. coli and characterized dipeptidase E activity using the Asp-ρNA substrate. The optimal pH and temperature for Orf2 are 7.5 and 40 ℃; Vmax and Km of Orf2 are 0.0787 mM·min−1 and 1.709 mM, respectively. Orf2 exhibits significant degradation activities to Asp-Gly-Gly, Asp-Leu, Asp-His, and isoAsp-Leu and minimal activities to Asp-Pro and Asp-Ala. Orf2 contains a Ser-His-Asp catalytic triad characterized by point mutation. In addition, the Asp147 residue of Orf2 is also proven to be critical for the enzyme’s activity through molecular docking and point mutation. Transcriptome analysis reveals the upregulation of genes associated with ribosomes, amino acid biosynthesis, and aminoacyl-tRNA biosynthesis in the orf2 mutant strain. Compared with the orf2 mutant strain and WT, the yield of crude polysaccharide does not change significantly. However, crude polysaccharides from the orf2 mutant strain exhibit a wider range of molecular weight distribution. The results indicate that the Orf2 links nutrient stress to secondary metabolism as a novel dipeptidase E. Key points • A novel dipeptidase E with a Ser-His-Asp catalytic triad was characterized from Streptomyces sp. 139. • Orf2 was involved in peptide metabolism both in vitro and in vivo. • Orf2 linked nutrient stress to mycelia formation and secondary metabolism in Streptomyces.

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Exposure to Al₂O₃ nanoparticles facilitates conjugative transfer of antibiotic resistance genes from Escherichia coli to Streptomyces

Cited by 31 publications

References 41 publications

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

Metallic Nanoparticles—Friends or Foes in the Battle against Antibiotic-Resistant Bacteria?

On the Cattaneo–Christov Heat Flux Model and OHAM Analysis for Three Different Types of Nanofluids

Characterization and functional evidence for Orf2 of Streptomyces sp. 139 as a novel dipeptidase E

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