Exposure to acids changes the proteomic of acquired dentine pellicle

Delecrode, Taísa Ribas; Siqueira, Walter L.; Zaidan, F.C.; Bellini, Melina Rodrigues; Leite, Aline L.; Xiao, Yishi; Ríos, Daniela; Magalhães, Ana Carolina; Buzalaf, Marília Afonso Rabelo

doi:10.1016/j.jdent.2015.02.001

Cited by 24 publications

(34 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…We employed an in vivo design that had been used before to evaluate proteins in the AEP that are resistant to removal by acids that mimic extrinsic erosion (citric acid) and caries (lactic acid) [Delecrode et al, 2015b]. Thus, the results obtained here are more realistic than those described in studies involving in vitro [Siqueira et al, 2012a;Masson et al, 2013;Custodio et al, 2015] or in situ [Delecrode et al, 2015a;Delius et al, 2017] protocols. Since our study was conducted in vivo, it was not possible to use other methods of collection, such as sonication or treating the samples with strong detergents that are cytotoxic, such as Triton X-100 or SDS, as has been done in in situ studies [Delius et al, 2017].…”

Section: Discussionmentioning

confidence: 99%

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

et al. 2018

Self Cite

View full text Add to dashboard Cite

Objective: Changes in the protein profile of acquired enamel pellicles (AEP) formed in vivo over different time periods were evaluated after the application of hydrochloric acid (HCl). Methods: Nine subjects were submitted to dental prophylaxis with pumice. After 3 or 120 min, the teeth were isolated with cotton rolls and 50 μL of 0.1 M HCl (pH 1.0), 0.01 M HCl (pH 2.0), or deionized water were applied on the buccal surface of the teeth for 10 s. The AEP was then collected using an electrode filter paper presoaked in 3% citric acid. After protein extraction, the samples were submitted to reverse-phase liquid chromatography coupled to mass spectrometry (nano LC-ESI-MS/MS). Label-free quantification was performed (Protein Lynx Global Service software). Results: A total of 180 proteins were successfully identified in the AEP samples. The number of identified proteins increased with the time of pellicle formation. Only 4 proteins were present in all the groups (isoforms of IgA, serum albumin, and statherin). The greatest number of proteins identified uniquely in one of the groups was obtained for the groups treated with HCl after 2 h of pellicle formation (approx. 50 proteins). Conclusion: Proteins resistant to removal by HCl, such as serum albumin and statherin, were identified even in the short-term AEP. In addition, 120-min pellicles present many proteins that are resistant to removal by HCl. This suggests an increase in protection against intrinsic acids with the time of pellicle formation, which should be evaluated in future studies.

show abstract

Section: Discussionmentioning

confidence: 99%

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

et al. 2018

Self Cite

View full text Add to dashboard Cite

show abstract

“…In situ protocols have also been employed in studies involving proteomic analysis of the acquired pellicle. The in situ models are particularly useful for some conditions that cannot be easily reproduced in vivo, such as the acquired pellicle formed over dentine specimens [Delecrode et al, 2015b]. Another advantage of in situ models is that they allow the use of sonication for pellicle collection, which makes total recovery of the pellicle proteins easier [Delius et al, 2017].…”

Section: Discussionmentioning

confidence: 99%

Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study

et al. 2018

Self Cite

View full text Add to dashboard Cite

This study detected changes in the protein profile of the acquired enamel pellicle (AEP) formed in vivo after rinsing with whole milk, fat-free milk, or water. Nine subjects in good oral condition took part in the study. The acquired pellicle was formed in the morning, for 120 min, after prophylaxis with pumice. Following this, the volunteers rinsed with 10 mL of whole milk, fat-free milk, or deionized water for 30 s, following a blinded crossover protocol. After 60 min, the pellicle was collected with filter paper soaked in 3% citric acid and processed for analysis by liquid chromatography-electrospray ionization tandem mass spectrometry. The obtained tandem mass spectrometry spectra were searched against a human protein database (Swiss-Prot). The proteomic data related to protein quantification were analysed using the PLGS software. A total of 260 proteins were successfully identified in the AEP samples collected from all groups. Forty-nine were common to all 3 groups, while 72, 62, and 49 were specific to the groups rinsing with whole milk, fat-free milk, and water, respectively. Some were typical components of the AEP, such as cystatin-B, cystatin-SN, isoforms of α-amylase, IgA and IgG, lysozyme C, protein S100 A78, histatin-1, proline-rich protein 27, statherin, and lactotransferrin. Other proteins are not commonly described as part of the AEP but could act in defence of the organism against pathogens. Distinct proteomic profiles were found in the AEP after rinsing with whole or fat-free milk, which could have an impact on bacterial adhesion and tooth dissolution. The use of fat-free milk could favourably modulate the adhesion of bacteria to the AEP as well as biofilm formation when compared with whole milk.

show abstract

“…So far, there is only scarce information about the pellicle proteome. Several studies focused on the characterization of the 2‐h biofilm proteome using pooled samples from several individuals and alternative elution protocols to obtain enough material for the subsequent MS‐analysis . There is one work of Lee et al.…”

Section: Introductionmentioning

confidence: 99%

Proteomic Analysis of the Initial Oral Pellicle in Caries‐Active and Caries‐Free Individuals

Trautmann

Barghash

Fecher‐Trost

et al. 2018

Proteomics Clinical Apps

View full text Add to dashboard Cite

Purpose: To 1) elucidate individual proteomic profiles of the 3-min biofilm of caries-active and caries-free individuals and 2) compare these proteomic profiles against the background of caries. Experimental design: The initial oral pellicle of 12 caries-active and 12 caries-free individuals is generated in situ on ceramics specimens. The individual, host-specific proteomic profiles of this basic pellicle layer are analyzed by a chemical elution protocol combined with an elaborate mass spectrometry and evaluated bioinformatically. Results: A total of 1188 different proteins are identified. Additionally, 68 proteins are present in the profiles of all individuals, suggesting them as ubiquitously occurring base-proteins of the initial human pellicle. Thereof, the single profiles exhibit high inter-individual differences independent of their group affiliation, stating the initial pellicle to represent a rather "individual fingerprint". Quantitative analyses imply slight indication for 23 proteins potentially capable of counting for caries-specific biomarkers. Conclusions and clinical relevance: The introduced protocol enables the individual analysis of minimal protein amounts and allows for highly precise characterizations and comparisons of individual proteomic profiles. The results contain a considerable higher extent of protein identifications and might serve as a base for future large scale analyzes to identify discrimination factors for the development of caries susceptibility tests.

show abstract

Exposure to acids changes the proteomic of acquired dentine pellicle

Cited by 24 publications

References 30 publications

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

Changes in the Proteomic Profile of Acquired Enamel Pellicles as a Function of Their Time of Formation and Hydrochloric Acid Exposure

Protein Profile of the Acquired Enamel Pellicle after Rinsing with Whole Milk, Fat-Free Milk, and Water: An in vivo Study

Proteomic Analysis of the Initial Oral Pellicle in Caries‐Active and Caries‐Free Individuals

Contact Info

Product

Resources

About