Exposure to a high dose of amoxicillin causes behavioral changes and oxidative stress in young zebrafish

Gonçalves, Cinara L.; Vasconcelos, Francine F P; Wessler, Leticia B.; Lemos, Isabela S; Candiotto, Gabriela; Lin, Jaime; Matias, Mariane B D; Rico, Eduardo Pacheco; Streck, Emílio L.

doi:10.1007/s11011-020-00610-6

Cited by 19 publications

(4 citation statements)

References 77 publications

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“…Zebrafish larvae are often used to study the neurotoxicity of aquatic pollutants. Several antibiotics have a negative impact on the locomotor activity of zebrafish [ 48 ]. Here, the visual motor response test revealed hyperactivity in the first light period at 5000 µg/L.…”

Section: Discussionmentioning

confidence: 99%

Sulfamethoxazole (SMX) Alters Immune and Apoptotic Endpoints in Developing Zebrafish (Danio rerio)

Iftikhar

Konig

English

et al. 2023

Toxics

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Sulfamethoxazole (SMX) is a broad-range bacteriostatic antibiotic widely used in animal and fish farming and is also employed in human medicine. These antibiotics can ultimately end up in the aquatic ecosystem and affect non-target organisms such as fish. To discern the effect of SMX on developing zebrafish embryos and larvae, we investigated a broad range of sub-lethal toxicity endpoints. Higher concentrations of SMX affected survivability, caused hatch delay, and induced malformations including edema of the yolk sac, pericardial effusion, bent tail, and curved spine in developing embryos. Lower levels of SMX provoked an inflammatory response in larvae at seven days post fertilization (dpf), as noted by up-regulation of interferon (ifn-γ) and interleukin 1β (il-1β). SMX also increased the expression of genes related to apoptosis, including BCL2-Associated Agonist of Cell Death (bad) and BCL2 Associated X, Apoptosis Regulator (bax) at 50 µg/L and decreased caspase 3 (casp3) expression in a dose-dependent manner. SMX induced hyperactivity in larval fish at 500 and 2500 µg/L based upon the light/dark preference test. Collectively, this study revealed that exposure to SMX can disrupt the immune system by altering host defense mechanisms as well as transcripts related to apoptosis. These data improve understanding of antibiotic chemical toxicity in aquatic organisms and serves as a baseline for in-depth environmental risk assessment of SMX and antibiotics.

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Section: Discussionmentioning

confidence: 99%

Sulfamethoxazole (SMX) Alters Immune and Apoptotic Endpoints in Developing Zebrafish (Danio rerio)

Iftikhar

Konig

English

et al. 2023

Toxics

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“…Lincomycin also has neurotoxic effects, reducing ventricular volume and neuronal numbers, but increasing systemic oxidative stress and apoptosis in zebrafish larvae, activating their acetylcholinesterase and ATPase, and decreasing locomotor activity [ 48 ]. In adult zebrafish, amoxicillin reduces locomotor and social behavior, and promotes oxidative stress in the brain, strikingly paralleling some clinical symptoms observed in autistic patients ( Figure 1 ) [ 49 ].…”

Section: Reported Cns Effects Of Antimicrobial Drugs In Animal Modelsmentioning

confidence: 97%

“…( B ) Exposure to oxytetracycline (10–100 mg/L) for 96 h evokes anxiety-like behavior in the novel tank test [ 61 ]. ( C ) Exposure to amoxicillin (100 mg/L) for 7 days reduces distance travelled and social interaction [ 49 ]. ( D ) Chronic exposure to β-diketones (at 6.25 mg/L) increases time spent at the top of the test tank (an anxiolytic-like behavior) and alters (increases at 6.25, and decreases at 25 mg/L) shoaling behavior [ 41 ].…”

Section: Figurementioning

confidence: 99%

Understanding CNS Effects of Antimicrobial Drugs Using Zebrafish Models

Kotova

Galstyan

Колесникова

et al. 2023

Veterinary Sciences

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Antimicrobial drugs represent a diverse group of widely utilized antibiotic, antifungal, antiparasitic and antiviral agents. Their growing use and clinical importance necessitate our improved understanding of physiological effects of antimicrobial drugs, including their potential effects on the central nervous system (CNS), at molecular, cellular, and behavioral levels. In addition, antimicrobial drugs can alter the composition of gut microbiota, and hence affect the gut–microbiota–brain axis, further modulating brain and behavioral processes. Complementing rodent studies, the zebrafish (Danio rerio) emerges as a powerful model system for screening various antimicrobial drugs, including probing their putative CNS effects. Here, we critically discuss recent evidence on the effects of antimicrobial drugs on brain and behavior in zebrafish, and outline future related lines of research using this aquatic model organism.

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“…In contrast, Tian et al showed that administration of typical fluoroquinolone antibiotics (Enrofloxacin) via incubation induced anxiety-like behaviors and gut dysbiosis demonstrated by increased Bacteroidetes and a decrease in the F/B ratio in the gut of adult zebrafish (Tian et al, 2023). Similarly, decreased social interaction and altered locomotion was observed following administration of Amoxicillin, a broad spectrum antibiotic previously associated with decreased richness in gut bacteria, in zebrafish (Deprey, 2016;Gonçalves et al, 2020). Zebrafish have also been used to model an intricate neuropsychiatric illness schizophrenia by studying shoaling behavior (Ewald, 2009), which can also be affected by the gut bacteria (Borrelli et al, 2016).…”

Section: Zebrafish As a Model To Explore The Microbiota-neural Axismentioning

confidence: 99%

Small fish, big discoveries: zebrafish shed light on microbial biomarkers for neuro-immune-cardiovascular health

et al. 2023

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Zebrafish (Danio rerio) have emerged as a powerful model to study the gut microbiome in the context of human conditions, including hypertension, cardiovascular disease, neurological disorders, and immune dysfunction. Here, we highlight zebrafish as a tool to bridge the gap in knowledge in linking the gut microbiome and physiological homeostasis of cardiovascular, neural, and immune systems, both independently and as an integrated axis. Drawing on zebrafish studies to date, we discuss challenges in microbiota transplant techniques and gnotobiotic husbandry practices. We present advantages and current limitations in zebrafish microbiome research and discuss the use of zebrafish in identification of microbial enterotypes in health and disease. We also highlight the versatility of zebrafish studies to further explore the function of human conditions relevant to gut dysbiosis and reveal novel therapeutic targets.

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Exposure to a high dose of amoxicillin causes behavioral changes and oxidative stress in young zebrafish

Cited by 19 publications

References 77 publications

Sulfamethoxazole (SMX) Alters Immune and Apoptotic Endpoints in Developing Zebrafish (Danio rerio)

Sulfamethoxazole (SMX) Alters Immune and Apoptotic Endpoints in Developing Zebrafish (Danio rerio)

Understanding CNS Effects of Antimicrobial Drugs Using Zebrafish Models

Small fish, big discoveries: zebrafish shed light on microbial biomarkers for neuro-immune-cardiovascular health

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