Exposure to a glyphosate-based herbicide formulation, but not glyphosate alone, has only minor effects on adult rat testis

Abstract

This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. Exposure to a glyphosate-based herbicide formulation, but not glyphosate alone, has only minor effects on adult rat testis Running Title: Low dose glyphosate exposure has little effect on adult rat testis.

Effects of parental exposure to glyphosate-based herbicides on embryonic development and oxidative status: a long-term experiment in a bird model

“…Thus, our methodology could not distinguish between the alternative (paternal versus maternal) underlying mechanisms could have been completely infertile or show developmental arrest at an early stage. We hypothesized that potential infertility may result from GBH-related problems in semen quality or altered reproductive behavior in males as suggested previously in other vertebrates 2, [34][35][36] . The fact that the measured egg quality parameters appeared not to be affected by Table 2.…”

Parental effects via glyphosate-based herbicides in a bird model?

“…Thus, our methodology could not distinguish between the alternative (paternal versus maternal) underlying mechanisms. Potential infertility may result from GBH-related problems in semen quality or altered reproductive behavior in males as suggested previously in other vertebrates (Cai et al, 2017; Gill et al, 2018; Johansson et al, 2018; Romano et al, 2012). Egg quality was not affected by GBHs and thus the negative effects of GBHs on female folliculogenesis and ovary development, detected in previous studies (Alarcon et al, 2019; Hamdaoui et al, 2018; Schimpf et al, 2017) are unlikely.…”

Glyphosate-based herbicide formulations and reproductive toxicity in animals

“…It should be noted that one study ( Romano et al., 2012 ) reported an increase in serum androgen levels at a GBH treatment of 50 mg/kg bodyweight which the authors attributed to either their observed increase in LH or failure in the negative feedback mechanism. Another recent study of male rats treated with either oral gavage of glyphosate alone or GBH at 25 mg/kg bodyweight displayed no significant changes to intra-testicular androgen levels, but the GBH formulation resulted in a small effect on steroidogenic gene expression ( Johansson et al. 2018 ).…”

“…Rat studies have reported decreases in interstitial cells with GBH treatment at 50.4 mg/kg bodyweight but increases in interstitial space at 250 mg/kg bodyweight treatment of GBH due to invasion by inflammatory cells ( Ikpeme et al., 2012 ; Owagboriaye et al., 2017 ). Recently, a study of male rats exposed by oral gavage to either glyphosate or a GBH formulation at 25 mg/kg bodyweight revealed no significant changes to the histology of testes ( Johansson et al., 2018 ). Reduction in epididymal tissues was observed in rat and duck studies at treatments with glyphosate and GBH as low as 5 mg/kg bodyweight ( Oliveira et al., 2007 ; Ikpeme et al., 2012 ; Abarikwu et al., 2015 ).…”