Exposure to 1.8 GHz radiofrequency field modulates ROS in human HEK293 cells as a function of signal amplitude

Pooam, Marootpong; Jourdan, Nathalie; Aguida, Blanche; Dahon, Cyril; Baouz, Soria; Terry, Colin; Raad, Haider; Ahmad, Margaret

doi:10.1080/19420889.2022.2027698

Cited by 6 publications

(8 citation statements)

References 36 publications

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“…9 For instance, 15 min of exposure to 1.8 GHz carrier frequency has been shown to cause an increase in intracellular reactive oxygen species in HEK293 cells in culture, accompanied by altered gene expression of many redox-associated enzymes. 12 This study also demonstrated that many RF bioeffects display a degree of hormesis, defined as an adaptive response of cells and organisms to moderate and typically intermittent stress. These hormetic biological responses do not follow a linear dose− response curve that increase as a function of increasing signal amplitude, and instead, they follow a biphasic dose−response curve with high response levels at certain signal amplitudes and no measurable response at others, often referred to as "blind spots".…”

Section: ■ Introductionmentioning

confidence: 67%

“…These hormetic biological responses do not follow a linear dose− response curve that increase as a function of increasing signal amplitude, and instead, they follow a biphasic dose−response curve with high response levels at certain signal amplitudes and no measurable response at others, often referred to as "blind spots". 12 Finally, the EM effect from cold atmospheric plasma on cancer cells is observed even if the cells are irradiated through filters that block UV and infrared radiation, which may indicate that the effect is caused by waves from other parts of the spectrum, for example, gigahertz 13 or terahertz radiation. 14,15 In a more recent work, it has been shown that even if the cold atmospheric plasma discharge is encapsulated in the closed, glass, dielectric container, referred to as the discharge tube (DT), the DT treatment can "sensitize" the cancer cells to chemotherapeutics to a greater degree than the chemotherapeutic drug alone.…”

Section: ■ Introductionmentioning

confidence: 99%

“…This study also demonstrated that many RF bioeffects display a degree of hormesis, defined as an adaptive response of cells and organisms to moderate and typically intermittent stress. These hormetic biological responses do not follow a linear dose–response curve that increase as a function of increasing signal amplitude, and instead, they follow a biphasic dose–response curve with high response levels at certain signal amplitudes and no measurable response at others, often referred to as “blind spots” …”

Section: Introductionmentioning

confidence: 99%

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Electromagnetic Nature of Distant Interaction of the Atmospheric Pressure Helium Plasma Discharge Tube with Glioblastoma Cancer Cells

Zolotukhin,

Horkowitz,

Keidar

2024

ACS Appl. Mater. Interfaces

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Atmospheric pressure coaxial gaseous discharge tubes (DTs) with helium have demonstrated potential for in vitro inactivation or sensitization of glioblastoma cancer cells. Here, we study the effect of two configurations of the DT electrode system on its electromagnetic emissivity as well as other physical factors (heating and UV emission) that form in the vicinity of this device. We demonstrate that the configuration of the DT electrodes that concentrates the discharge streamers near the top of the device has a distant (cm scale) deactivation effect on U87-MG glioblastoma cancer cells when irradiated, without measurable UV components in the DT optical emission spectra. This effect persists even through different barriers such as glass, plastic, or quartz Petri dishes but is eliminated when glass or plastic dishes are filled with water. These findings demonstrate the potential for development of noninvasive, physical-based treatment methods of deep-tissue tumors.

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Section: ■ Introductionmentioning

confidence: 67%

Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Electromagnetic Nature of Distant Interaction of the Atmospheric Pressure Helium Plasma Discharge Tube with Glioblastoma Cancer Cells

Zolotukhin,

Horkowitz,

Keidar

2024

ACS Appl. Mater. Interfaces

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“…ROS generation in response to RF-EMF was also reported in human HEK293 cells where the 1.8 GHz field strongly modified the expression of antioxidative and oxidative enzymes [ 98 ]. The response of CRY1 to a magnetic field up to 1.32 mT under in vitro conditions has also been demonstrated [ 99 ].…”

Section: Discussionmentioning

confidence: 99%

2.4 GHz Electromagnetic Field Influences the Response of the Circadian Oscillator in the Colorectal Cancer Cell Line DLD1 to miR-34a-Mediated Regulation

Olejárová

Moravčík

Herichová

2022

IJMS

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Radiofrequency electromagnetic fields (RF-EMF) exert pleiotropic effects on biological processes including circadian rhythms. miR-34a is a small non-coding RNA whose expression is modulated by RF-EMF and has the capacity to regulate clock gene expression. However, interference between RF-EMF and miR-34a-mediated regulation of the circadian oscillator has not yet been elucidated. Therefore, the present study was designed to reveal if 24 h exposure to 2.4 GHz RF-EMF influences miR-34a-induced changes in clock gene expression, migration and proliferation in colorectal cancer cell line DLD1. The effect of up- or downregulation of miR-34a on DLD1 cells was evaluated using real-time PCR, the scratch assay test and the MTS test. Administration of miR-34a decreased the expression of per2, bmal1, sirtuin1 and survivin and inhibited proliferation and migration of DLD1 cells. When miR-34a-transfected DLD1 cells were exposed to 2.4 GHz RF-EMF, an increase in cry1 mRNA expression was observed. The inhibitory effect of miR-34a on per2 and survivin was weakened and abolished, respectively. The effect of miR-34a on proliferation and migration was eliminated by RF-EMF exposure. In conclusion, RF-EMF strongly influenced regulation mediated by the tumour suppressor miR-34a on the peripheral circadian oscillator in DLD1 cells.

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“…Halliwell and Gutteridge ( 21 ) show the importance of controlling the concentrations of radicals and H 2 O 2 in controlling biological processes to keep them in the normal operating range. Pooam et al ( 22 ) show that exposures at 1 GHz for 15 min can modulate ROS in human HEK293 cells as a function of signal amplitude, changed gene expression and anti-oxidative enzymes (SOD, GPX, GPX, and CAT) and oxidative (Nox-2) enzyme concentrations. Responses are non-linear in amplitude and are frequency dependent.…”

Section: Literature Summarymentioning

confidence: 99%

Some thoughts on the possible health effects of electric and magnetic fields and exposure guidelines

Barnes

Freeman

2022

Front. Public Health

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Concerns about the possible health effects from exposure to weak electric and magnetic (EM) fields have been debated since the early 1960s. It is now well established that biological systems respond to exposure to weak EM fields at energy levels well below the current safety guidelines which result in modification of their functionality without significant changes in temperature. These observations are adding to the debate over what should be done to protect the users of cellular telecommunications systems. Experimental results showing both increases and decreases in cancer cell growth rates and concentration of reactive oxygen species for exposure to nano-Tesla magnetic fields at both radio frequencies (RF) and extra low frequencies (ELF) are cited in this paper. Some theoretical models on how variations in EM exposure can lead to different biological outcomes and how feedback and repair processes often mitigate potential health effects due to long-term exposure to low-level EM energy sources are presented. Of particular interest are the application of the radical pair mechanisms that affect polarization of electrons, and nuclear spins and the importance of time-delayed feedback loops and the timing of perturbations to oscillations in biological systems. These models help account for some of the apparently conflicting experimental results reported and suggest further investigation. These observations are discussed with particular emphasis on setting future safety guidelines for exposure to electromagnetic fields in cellular telecommunications systems. The papers cited are a very small fraction of those in the literature showing both biological effects and no effects from weak electric and magnetic fields.

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Exposure to 1.8 GHz radiofrequency field modulates ROS in human HEK293 cells as a function of signal amplitude

Cited by 6 publications

References 36 publications

Electromagnetic Nature of Distant Interaction of the Atmospheric Pressure Helium Plasma Discharge Tube with Glioblastoma Cancer Cells

Electromagnetic Nature of Distant Interaction of the Atmospheric Pressure Helium Plasma Discharge Tube with Glioblastoma Cancer Cells

2.4 GHz Electromagnetic Field Influences the Response of the Circadian Oscillator in the Colorectal Cancer Cell Line DLD1 to miR-34a-Mediated Regulation

Some thoughts on the possible health effects of electric and magnetic fields and exposure guidelines

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