Exposure of the Opportunistic Marine Pathogen Photobacterium damselae subsp. damselae to Human Body Temperature Is a Stressful Condition That Shapes the Transcriptome, Viability, Cell Morphology, and Virulence

Abstract

Photobacterium damselae Response to 37 • C human body temperature constitutes a stressful condition for Pdd. This study provides the first transcriptome profile of Pdd exposed at human body temperature, and unveils a number of candidate molecular targets for prevention and control of human infections caused by this pathogen.

The two‐component system RstAB regulates production of a polysaccharide capsule with a role in virulence in the marine pathogen Photobacterium damselae subsp. damselae

“…However, the specific stimuli that trigger RstB autophosphorylation as such have not been identified so far. In addition, the conditions that influence transcription of rstAB genes are poorly investigated, although previous studies indicate that temperature changes do not affect expression of rstAB genes (Matanza and Osorio, 2018; Matanza and Osorio, 2020).…”

Mediterranean Aquaculture in a Changing Climate: Temperature Effects on Pathogens and Diseases of Three Farmed Fish Species

“…To our knowledge, with the exception of some data on Photobacterium damselae subsp. damselae [ 152 , 153 ], no Mediterranean aquaculture-related pathogen has been assessed using a molecular approach in relation to temperature changes. A realistic proxy to depict the pathways perturbed by temperature changes is the human pathogen Klebsiella pneumoniae ; this is an opportunistic pathogen in warm freshwater aquaculture, also propagated through contaminated seafood [ 154 ], particularly abundant in natural water reservoirs exposed to temperature variation.…”

“…An increase in temperature may also activate the ‘heat-shock response’ (with a consequent production of HSPs), a well-known mechanism that limits the damage caused by exposure to extreme temperatures [ 271 ]. The mechanism of this stress response is associated with impairment of cell viability, abnormal cell morphologies, changes in cell membrane lipid composition and downregulation of virulence factor expression [ 152 ].…”

“…Diseases caused by this bacterium in aquaculture have often been related to increased water temperatures during summer months, reflected also from the fact that both bacterial growth and expression levels of virulence factors are higher at 25 °C, than at 15 °C [ 153 ]. A recent study investigating transcriptome differences in bacteria grown at 25 and 37 °C suggested that the latter temperature activates a strong stress response that impairs their growth, viability, morphology and virulence [ 152 ]. P. damselae subsp.…”

Comparative analysis of spleen transcriptome of immune response in Sebastes schlegeli against Photobacterium damselae subsp. damselae infection

“…Transcriptome analysis on fish immune responses during bacterial infection has been carried out on different kinds of fish infected with various pathogens (Jiang et al, 2016;Jiao et al, 2020;Tran et al, 2018) and provided an effective approach for assessing the pathogen-host interactions without prior knowledge of the reference genomic DNA sequence. For studies on Photobacteriosis caused by Pdd, Matanza and Osorio used RNA sequencing to analyse the transcriptome regulatory changes in Pdd triggered by the temperature shift, which were the only report so far (Matanza & Osorio, 2020;Matanza et al, 2018). However, there is no information available on the gene expression profile of the whole transcriptome or its response to Pdd infection in marine animals, which could contribute to our understanding of the molecular responses in S. schlegeli.…”