Exposure of rats to a 50-Hz, 100 ?Tesla magnetic field does not affect the ex vivo production of interleukins by activated T or B lymphocytes

M, Häussler; Thun-Battersby, Susanne; Mevissen, Meike; Löscher, Wolfgang

doi:10.1002/(sici)1521-186x(1999)20:5<295::aid-bem6>3.0.co;2-p

Cited by 4 publications

(1 citation statement)

References 45 publications

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“…The exposure system was built by the Department of Biophysics (Iran University of Medical Sciences) as previously described in detail [15]. The PEMFs were generated by a pair of Helmholtz coils having a diameter of 75 cm.…”

Section: Methodsmentioning

confidence: 99%

Effects of extremely‐low‐frequency pulsed electromagnetic fields on collagen synthesis in rat skin

Ahmadian

Zarchi

Bolouri

2006

Biotech and App Biochem

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To investigate the effects of extremely-low-frequency PEMFs (pulsed electromagnetic fields) on the synthesis of epidermal collagen, six groups of animals each consisting of eight mature male rats were selected randomly: one group for the control and five for the test. Using a parallel set of Helmholtz coils, a uniform field intensity of 2 mT at different frequencies of 25, 50 and 100 Hz yielded the most effective frequency to be 25 Hz. Then, at this frequency, two different field intensities of 1 and 4 mT were applied. The treatment time of 2.5 h/day lasted for 8 days, keeping the same procedure for the control group, except with the field turned off. On the ninth day, the rats were killed and skin samples from the dorsal region were taken for collagen assessment by measuring hydroxyproline content using the Stegemann-Stalder [(1967) Clin. Chim. Acta 8, 267-273] method. The results indicated that a PEMF of 2 mT at 25 Hz increased the collagen synthesis (P < 0.05). The other intensities and frequency setting did not have any noticeable effect; however, at a frequency of 25 Hz at 4 mT, collagen increase was also noticed. It was concluded that at 25 Hz under a field setting of 2 mT for the duration of 8 days, stimulation of skin at 2.5 h/day would cause increase in collagen synthesis in rat skin.

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Section: Methodsmentioning

confidence: 99%

Effects of extremely‐low‐frequency pulsed electromagnetic fields on collagen synthesis in rat skin

Ahmadian

Zarchi

Bolouri

2006

Biotech and App Biochem

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Factors confounding cytosolic calcium measurements in Jurkat E6.1 cells during exposure to ELF magnetic fields

McCreary

Thomas

Prato

2002

Bioelectromagnetics

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Reported changes in the cytosolic calcium concentration ([Ca2+](c)) as a result of exposure to extremely low frequency (ELF) magnetic fields (MF) have been equivocal. In this study, we examine the possibility that some of these differences are attributable to variability associated with the cell cycle, pH of the suspension medium, and response to a calcium agonist. We used a custom designed spectrofluorimeter to measure [Ca2+](c) in Indo 1-AM loaded Jurkat E6.1 cells suspended in conditioned RPMI 1640 medium containing 10% fetal bovine serum. Four exposures were examined: zero static MF (Null), 60 Hz 100 microT(peak) sinusoidal MF (AC), 78 microT static MF (DC), and the combination of the 60 Hz and the 78 microT static MF (AD + DC). A significant decrease in normalized [Ca2+](c) values between 375-495 s for the DC and AC + DC groups was found in comparison to the Null group. However, statistical analysis indicated that cell cycle and quality of the alpha-CD3 monoclonal antibody response were significant covariates, while pH was not a significant covariate. When the effect of these covariates was taken into account, all exposure groups were significantly different from the control. Our results suggest that ELF MF effects may not be seen unless correction is made for biological variability of each cell preparation with respect to cell cycle and [Ca2+](c) response to antigen stimulation.

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Experimental Results: In vivo

Κato

Electromagnetics in Biology

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Exposure of rats to a 50-Hz, 100 ?Tesla magnetic field does not affect the ex vivo production of interleukins by activated T or B lymphocytes

Cited by 4 publications

References 45 publications

Effects of extremely‐low‐frequency pulsed electromagnetic fields on collagen synthesis in rat skin

Effects of extremely‐low‐frequency pulsed electromagnetic fields on collagen synthesis in rat skin

Factors confounding cytosolic calcium measurements in Jurkat E6.1 cells during exposure to ELF magnetic fields

Experimental Results: In vivo

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