Exposure of pink salmon embryos to dissolved polynuclear aromatic hydrocarbons delays development, prolonging vulnerability to mechanical damage

Carls, Mark G.; Thedinga, John F.

doi:10.1016/j.marenvres.2009.12.006

Cited by 36 publications

(28 citation statements)

References 40 publications

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“…We found that mortality significantly increased in the F1 generation after parents (F0) had been fed medium and high doses of BaP. This result was consistent with previous findings that waterborne exposure to PAHs in embryos and larvae increased mortality (Carls and Thedinga, 2010; Barron et al, 2004; Hawkins et al, 2002; Carls et al, 1999). The same was true when zebrafish embryos were exposed to BaP alone (Fang et al, 2013; Bugiak et al, 2010).…”

Section: Discussionsupporting

confidence: 92%

“…Because maternal BaP deposition from diet in F1 embryos was undetected, the embryo exposure may be most similar to lower PAH concentration exposures (waterborne or sediment) in embryos where time to hatch also decreased in Pacific herring, fathead minnow, and pink salmon embryos (Carls et al, 1999; Colavecchia et al, 2004; Carls and Thedinga, 2010). On the other hand, after direct exposure to higher PAH concentrations time to hatch increased (delayed hatching) (Colavecchia et al, 2004; Carls and Thedinga, 2010). Time to hatch also increased (delayed hatching) after a direct waterborne exposure to BaP alone (0.24 and 24 μg/L) (Fang et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…Cardiac deformities were also apparent after BaP exposure in the absence of other PAHs (Wills et al, 2009). In pink salmon ( Oncorhynchus gorbuscha ) and rainbow trout ( O. mykiss ), dissolved PAHs from crude oil caused higher mortality, alterations in time to hatch, growth reduction, spinal deformities, jaw deformities, yolk sac edema, and impaired swimming (Carls and Thedinga, 2010; Barron et al, 2004; Hawkins et al, 2002). In Sebastiscus marmoratus , a teleost of the scorpion fish family, skeletal deformities including incidence of spinal curvature and craniofacial defects were caused by BaP as well as altered expression of genes involved in bone formation (He et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

et al. 2014

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In the aquatic environment, adverse outcomes from dietary polycyclic aromatic hydrocarbon (PAH) exposure are poorly understood, and multigenerational developmental effects following exposure to PAHs are in need of exploration. Benzo[a]pyrene (BaP), a model PAH, is a recognized carcinogen and endocrine disruptor. Here adult zebrafish (F0) were fed 0, 10, 114, or 1012 μg BaP/g diet at a feed rate of 1% body weight twice/day for 21 days. Eggs were collected and embryos (F1) were raised to assess mortality and time to hatch at 24, 32, 48, 56, 72, 80, and 96 hours post fertilization (hpf) before scoring developmental deformities at 96 hpf. F1 generation fish were raised to produce the F2 generation followed by the F3 and F4 generations. Mortality significantly increased in the higher dose groups of BaP (2.3 and 20 μg BaP/g fish) in the F1 generation while there were no differences in the F2, F3, or F4 generations. In addition, premature hatching was observed among the surviving fish in the higher dose of the F1 generation, but no differences were found in the F2 and F3 generations. While only the adult F0 generation was BaP-treated, this exposure resulted in multigenerational phenotypic impacts on at least two generations (F1 and F2). Body morphology deformities (shape of body, tail, and pectoral fins) were the most severe abnormality observed, and these were most extreme in the F1 generation but still present in the F2 but not F3 generations. Craniofacial structures (length of brain regions, size of optic and otic vesicles, and jaw deformities), although not significantly affected in the F1 generation, emerged as significant deformities in the F2 generation. Future work will attempt to molecularly anchor the persistent multigenerational phenotypic deformities noted in this study caused by BaP exposure.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

et al. 2014

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“…The effects of PAH exposure on fish embryos are well characterized and include increased mortality (Carls et al ; Heintz et al ; Carls and Thedinga ; Hedgpeth and Griffitt ), delayed or reduced hatching (Dubansky et al ; Hedgpeth and Griffitt ), and developmental abnormalities (Carls et al ; Dubansky et al ; Incardona et al , ; Mager et al ). Our data corroborate these findings because all the groups in the present study displayed an approximate PAH dose‐dependent relationship for hatching and mortality data (Figure A and B).…”

Section: Discussionmentioning

confidence: 99%

Combined effects of Deepwater Horizon crude oil and environmental stressors on Fundulus grandis embryos

Rodgers

Jones

Klinkhamer³

et al. 2018

Enviro Toxic and Chemistry

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In the present study, we examined how sensitivity to oil changes in combination with environmental stressors in Fundulus grandis embryos. We exposed embryos (<24 h post fertilization) to a range of high-energy water accommodated fraction (HEWAF) concentrations (0-50 parts per billion [ppb] total polycyclic aromatic hydrocarbons [PAHs]) made from Macondo crude oil in conjunction with various environmental conditions (temperature: 20 and 30 °C; salinity: 3, 7, and 30 practical salinity units [PSU]; and dissolved oxygen: 2 and 6 mg/L). Endpoints included mortality, hatching rates, and expression of cytochrome p450 1a and 1c (cyp1a, cyp1c) in hatched larvae. There was 100% mortality for all fish under the 2 parts per million (ppm) dissolved oxygen regimes. For the 6 mg/L dissolved oxygen treatments, mortality and median lethal time (LT50) were generally higher in the 30 °C treatments versus the 20 °C treatments. Oil increased mortality in fish exposed to the highest concentration in the 20-3-6 (°C-PSU-mg/L), 25-7-6, and 30-30-6 conditions. Hatching was driven by environmental conditions, with oil exposure having a significant impact on hatching in only the 25-7-6 and 30-30-6 groups at the greatest HEWAF exposure. Expression of cyp1a was up-regulated in most treatment groups versus the controls, with cyp1c expression exhibiting a similar pattern. These data suggest interactive effects among temperature, salinity, and PAHs, highlighting a need to further assess the effects of oil exposure under various environmental conditions. Environ Toxicol Chem 2018;37:1916-1925. © 2018 SETAC.

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“…In the case of marine oil spills, such as the Deepwater Horizon spill, there is an initial, acute risk to organisms that can become covered in viscous crude as well as acute and chronic risks from exposure to toxic chemicals through air, water and food. Even after the oil is no longer visible, chemicals of concern can persist in the environment [6] and affect exposed organisms [5, 7]. It is the freely dissolved fraction of chemicals in the water that is bioavailable to diffuse across biological membranes and enter organisms and the food web [8].…”

Section: Introductionmentioning

confidence: 99%

Impact of the Deepwater Horizon Oil Spill on Bioavailable Polycyclic Aromatic Hydrocarbons in Gulf of Mexico Coastal Waters

Allan

Smith

Anderson

2012

Environ. Sci. Technol.

322

204

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An estimated 4.1 million barrels of oil and 2.1 million gallons of dispersants were released into the Gulf of Mexico during the Deepwater Horizon oil spill. There is a continued need for information about the impacts and long-term effects of the disaster on the Gulf of Mexico. The objectives of this study were to assess bioavailable polycyclic aromatic hydrocarbons (PAHs) in the coastal waters of four Gulf Coast states that were impacted by the spill. For over a year, beginning in May 2010, passive sampling devices were used to monitor the bioavailable concentration of PAHs. Prior to shoreline oiling, baseline data were obtained at all the study sites, allowing for direct before and after comparisons of PAH contamination. Significant increases in bioavailable PAHs were seen following the oil spill, however, pre-oiling levels were observed at all sites by March, 2011. A return to elevated PAH concentrations, accompanied by a chemical fingerprint similar to that observed while the site was being impacted by the spill, was observed in Alabama in summer, 2011. Chemical forensic modeling demonstrated that elevated PAH concentrations are associated with distinctive chemical profiles.

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Exposure of pink salmon embryos to dissolved polynuclear aromatic hydrocarbons delays development, prolonging vulnerability to mechanical damage

Cited by 36 publications

References 40 publications

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

Multigenerational effects of benzo[a]pyrene exposure on survival and developmental deformities in zebrafish larvae

Combined effects of Deepwater Horizon crude oil and environmental stressors on Fundulus grandis embryos

Impact of the Deepwater Horizon Oil Spill on Bioavailable Polycyclic Aromatic Hydrocarbons in Gulf of Mexico Coastal Waters

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