Exposure of liver cell culture from the orange-spotted grouper, Epinephelus coioides, to benzo[a]pyrene and light results in oxidative damage as measured by antioxidant enzymes

Derakhshesh, Negin; Salamat, Negin; Movahedinia, Abdolali; Hashemitabar, Mahmoud; Bayati, Vahid

doi:10.1016/j.chemosphere.2019.03.181

Cited by 23 publications

(16 citation statements)

References 61 publications

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“…The toxicity of BaP towards organisms has been widely reported using colon epithelial cells as an in vitro model system [20,21]. Derakhshesh et al used MTT assay to indicate that a concentration of 100 µM BaP would lead more than 50% of the intestinal cell lines to die [22]. In this study, a concentration of 50 µM BaP was observed to strongly inhibit the growth of 52.78% colon epithelial cells, according to MTT assay.…”

Section: Discussionmentioning

confidence: 62%

“…Recently, several studies have explored the efficiency of flavonoids and butyrates in weakening the cytotoxicity of BaP using different cell lines. It was seen that flavonoids did not decrease AhR expression but counteracted BaP-mediated aryl hydrocarbon receptor repressor (AhRR) repression [22,34,35]. It was found that butyrate not only altered the expression of cytochrome CYP1A1 and metabolism of BaP via histone deacetylase activity but also improved the expression and activity of xenobiotic enzymes, which are responsible for metabolizing carcinogens in the colon epithelial cell model.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Zhang

et al. 2020

Toxics

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Long-term exposure to benzo(a)pyrene (BaP) poses a serious genotoxic threat to human beings. This in vitro study investigated the potential of inactivated Bifidobacterium animalis subsp. lactis BI-04 in alleviating the damage caused by BaP in colon epithelial cells. A concentration of BaP higher than 50 μM strongly inhibited the growth of colon epithelial cells. The colon epithelial cells were treated with 50 μM BaP in the presence or absence of inactivated strain BI-04 (~5 × 108 CFU/mL). The BaP-induced apoptosis of the colon epithelial cells was retarded in the presence of B. lactis BI-04 through activation of the PI3K/ AKT signaling pathway, and p53 gene expression was decreased. The presence of the BI-04 strain reduced the intracellular oxidative stress and DNA damage incurred in the colon epithelial cells by BaP treatment due to the enhanced expression of antioxidant enzymes and metabolism-related enzymes (CYP1A1). The data from comet assay, qRT-PCR, and western blot analysis showed that the cytotoxic effects of BaP on colon epithelial cells were largely alleviated because the bifidobacterial strain could bind to this carcinogenic compound. The in vitro study highlights that the consumption of commercial probiotic strain BI-04 might be a promising strategy to mitigate BaP cytotoxicity.

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Section: Discussionmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Zhang

et al. 2020

Toxics

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“…Usually, ALT and AST are considered as the biomarker for injury to liver (Derakhshesh et al. 2019 ). The MDA is one of the degradative products of polyunsaturated fatty acid peroxide, which can cause cross-linking polymerization of nucleic acid, protein and other endogenous macromolecules, as well as induce cytotoxicity (Xie et al.…”

Section: Discussionmentioning

confidence: 99%

Jiang Zhi Granule protects immunological barrier of intestinal mucosa in rats with non-alcoholic steatohepatitis

Zhang

Pan

et al. 2021

Pharmaceutical Biology

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Context Jiang Zhi Granule (JZG) is known to improve hepatic function, reduce liver fat deposition and inflammation in non-alcoholic fatty liver disease (NAFLD). Objective To determine the protective mechanism of JZG on immunological barrier of intestinal mucosa in rats with diet-induced non-alcoholic steatohepatitis (NASH). Materials and methods A Sprague-Dawley (SD) model of NASH was established using a high-fat diet and 1% dextran sulphate sodium (DSS) through drinking water. The rats were randomized into four groups and treated for four weeks, respectively, including normal control (NC), model control (MC), positive control (PC) and JZG. Mesenteric lymph nodes (MLNs) cells were isolated and cultured to assess a potential disruption of the enteric immune barrier. Also, investigation of intestinal mucosal dendritic cell-toll-like-receptor-myeloid differentiation primary response 88 (DC-TLR-MyD88) signalling pathway in vitro was examined. Results The lethal concentration 50 (LD 50 ) of JZG was greater than 5 g/kg, while its inhibitory concentration 50 (IC 50 ) was 1359 μg/mL in HepG2. In JZG group, the plasma levels of alanine transaminase (ALT), aspartate transaminase (AST), malondialdehyde (MDA), low-density lipoprotein cholesterol (LDL-C), total cholesterol (TC), triglyceride (TG) and serum endotoxin were significantly ( p < 0.01) reduced. In contrast, plasma concentrations of high - density lipoprotein cholesterol (HDL-C) and superoxide dismutase (SOD) were increased. Furthermore, proinflammatory factor, interferon-γ (IFN-γ)+ from CD4+ T cells in DSS-induced NASH rats increased significantly ( p < 0.01) compared to NC group. Importantly, JZG treatment substantially decreased ( p < 0.01) the relative expressions of TLR-44 and MyD88. Conclusions JZG treatment may protect immunological barrier of intestinal mucosa in NASH individual.

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“…B[a]P has a strong ability to enter and accumulate in the cells of aquatic organisms and causing various abnormalities and disorders due to its aromatic fused ring and lipophilicity (Derakhshesh et al, 2019). Previous studies showed that B[a]P is capable of influencing cell growth, effective quantum yield, and photosynthesis of phytoplankton (Li et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Combined Effects of Microplastics and Benzo[a]pyrene on the Marine Diatom Chaetoceros muelleri

Hou

et al. 2022

Front. Mar. Sci.

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Microplastics are regarded as ubiquitous pollutants in the ocean and have attracted worldwide concerns. Benzo[a]pyrene (B[a]P), one of typical polycyclic aromatic hydrocarbons (PAHs), is commonly detected in marine environment. Once coexisted, the microplastics and B[a]P may interact with each other and result in combined toxicity to organisms, and these remain to be systematically elucidated. Thus, this study aims to investigate (i) the effects of single micro-sized polystyrene (mPS), polyethylene terephthalate (mPET), and B[a]P on cell growth of Chaetoceros muelleri; and (ii) the interaction of microplastics and B[a]P, and their combined effects on C. muelleri. The results showed that both single microplastics and B[a]P at a high concentration of 150 μg/L inhibited the growth of C. muelleri. For single treatment of microplastics, stronger inhibition effects on microalgae was caused by mPET than that of mPS, with the highest IR of 25.23 and 11.17%, respectively. This may be attributed to the obvious surface roughness of mPET. By comparison, the combined effects of microplastics and B[a]P significantly inhibited the growth of C. muelleri as compared with the single treatment of B[a]P (P < 0.05). Synergistic effect was found in the combination of microplastics with B[a]P at high concentrations of 150 μg/L. Interestingly, the antagonistic effect on C. muelleri was observed in the combined treatment of microplastics and B[a]P at low concentrations of 10 μg/L. In addition, the pollutants reduced the content of photosynthetic pigments in microalgal cells. The SOD and MDA content of microalgae increased in the early stage of exposure to pollutants (e.g., Days 1 and 5), but decreased in the later stage (Day 15) compared with the control group. The decreased superoxide dismutase (SOD) and catalase (CAT) activity in single and composite systems may indicate that the antioxidative enzymatic system of microalgae has been inhibited or destroyed. This study will be helpful to further explore the ecological threats of microplastics and PAHs to the marine ecosystem.

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Exposure of liver cell culture from the orange-spotted grouper, Epinephelus coioides, to benzo[a]pyrene and light results in oxidative damage as measured by antioxidant enzymes

Cited by 23 publications

References 61 publications

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Potential of Inactivated Bifidobacterium Strain in Attenuating Benzo(A)Pyrene Exposure-Induced Damage in Colon Epithelial Cells In Vitro

Jiang Zhi Granule protects immunological barrier of intestinal mucosa in rats with non-alcoholic steatohepatitis

Combined Effects of Microplastics and Benzo[a]pyrene on the Marine Diatom Chaetoceros muelleri

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