Exposure of juvenile Leghorn chickens to lead acetate enhances antibiotic resistance in enteric bacterial flora

Nisanian, M.; Holladay, Steven D.; Karpuzoglu, Ebru; Kerr, Richard; Williams, Susan M.; Stabler, Lisa; McArthur, J. Vaun; Tuckfield, R. Cary; Gogal, Robert M.

doi:10.3382/ps.2013-03600

Cited by 13 publications

(13 citation statements)

References 22 publications

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“…Arsenic cycling has been suggested to be a prominent feature in ancient microbial mats over 2.7 billion years old ( Sforna et al, 2014 ). Our data from Pavilion Lake microbialites suggest that heavy-metal resistance could be a general feature of microbialites globally, which may also provide cross-protection against antibiotics ( Nisanian et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

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Metagenomic Analysis Suggests Modern Freshwater Microbialites Harbor a Distinct Core Microbial Community

et al. 2016

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Modern microbialites are complex microbial communities that interface with abiotic factors to form carbonate-rich organosedimentary structures whose ancestors provide the earliest evidence of life. Past studies primarily on marine microbialites have inventoried diverse taxa and metabolic pathways, but it is unclear which of these are members of the microbialite community and which are introduced from adjacent environments. Here we control for these factors by sampling the surrounding water and nearby sediment, in addition to the microbialites and use a metagenomics approach to interrogate the microbial community. Our findings suggest that the Pavilion Lake microbialite community profile, metabolic potential and pathway distributions are distinct from those in the neighboring sediments and water. Based on RefSeq classification, members of the Proteobacteria (e.g., alpha and delta classes) were the dominant taxa in the microbialites, and possessed novel functional guilds associated with the metabolism of heavy metals, antibiotic resistance, primary alcohol biosynthesis and urea metabolism; the latter may help drive biomineralization. Urea metabolism within Pavilion Lake microbialites is a feature not previously associated in other microbialites. The microbialite communities were also significantly enriched for cyanobacteria and acidobacteria, which likely play an important role in biomineralization. Additional findings suggest that Pavilion Lake microbialites are under viral selection as genes associated with viral infection (e.g CRISPR-Cas, phage shock and phage excision) are abundant within the microbialite metagenomes. The morphology of Pavilion Lake microbialites changes dramatically with depth; yet, metagenomic data did not vary significantly by morphology or depth, indicating that microbialite morphology is altered by other factors, perhaps transcriptional differences or abiotic conditions. This work provides a comprehensive metagenomic perspective of the interactions and differences between microbialites and their surrounding environment, and reveals the distinct nature of these complex communities.

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Section: Resultsmentioning

confidence: 99%

“…Heavy-metal resistance genes may be involved in resistance, homeostasis or sequestration of metals. Antibiotic resistance has also been linked to heavy-metal stress, suggesting that resistance to one can lead to resistance to the other in complex bacterial communities ( Nisanian et al, 2014 ).…”

Section: Resultsmentioning

confidence: 99%

Metagenomic Analysis Suggests Modern Freshwater Microbialites Harbor a Distinct Core Microbial Community

et al. 2016

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“…Many of these genes, however, are associated with conjugative transposons (e.g., Tn 917 and the Tn 916–1525 family), which are often associated with multiple resistance (Roberts 2012). Beta-lactam resistance has been associated with mercury (e.g., Fraser 1971; Richmond et al 1964) and copper (e.g., Knapp et al 2011) exposure, and lead exposure contributed to AR in enteric bacteria in poultry (Nisanian et al 2014). The results from our study indicate linkage with other metals, namely aluminium, manganese, uranium and lead.…”

Section: Resultsmentioning

confidence: 99%

Relationship between antibiotic resistance genes and metals in residential soil samples from Western Australia

Knapp

Callan

Aitken

et al. 2016

Environ Sci Pollut Res

147

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Increasing drug-resistant infections have drawn research interest towards examining environmental bacteria and the discovery that many factors, including elevated metal conditions, contribute to proliferation of antibiotic resistance (AR). This study examined 90 garden soils from Western Australia to evaluate predictions of antibiotic resistance genes from total metal conditions by comparing the concentrations of 12 metals and 13 genes related to tetracycline, beta-lactam and sulphonamide resistance. Relationships existed between metals and genes, but trends varied. All metals, except Se and Co, were related to at least one AR gene in terms of absolute gene numbers, but only Al, Mn and Pb were associated with a higher percentage of soil bacteria exhibiting resistance, which is a possible indicator of population selection. Correlations improved when multiple factors were considered simultaneously in a multiple linear regression model, suggesting the possibility of additive effects occurring. Soil-metal concentrations must be considered when determining risks of AR in the environment and the proliferation of resistance.

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“…Beyond studies of co-election in environmental media, studies on Staphylococcus isolates from animals and humans have shown associations between Pb and Cd resistance and antibiotic resistance [ 26 , 27 ]. For example, fecal bacterial isolates from leghorn chickens that were fed Pb at levels that did not cause other morbidity or mortality, showed significantly elevated levels of antibiotic resistance compared to controls, and demonstrated a dose response relationship within different levels of Pb in the diet [ 28 ]. However, to our knowledge, few if any studies have examined the relationship between Pb or Cd exposure and selection for antibiotic resistance in humans.…”

Section: Introductionmentioning

confidence: 99%

Heavy metal exposure and nasal Staphylococcus aureus colonization: analysis of the National Health and Nutrition Examination Survey (NHANES)

2018

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BackgroundHeavy metals including lead and cadmium can disrupt the immune system and the human microbiota. and are increasingly of concern with respect to the propogation of antibiotic-resistence. Infection by methicillin-resistant Staphylococcus aureus (MRSA) is a major cause of global morbidity and mortality. Heavy metal exposure may be associated with increased MRSA colonization and infection, and a decrease in methicillin-susceptible Staphylococcus aureus (MSSA) through co-selection mechanisms and natural selection of antibiotic resistance in the presence of heavy metals. This study examines the association between blood lead (Pb) and cadmium (Cd) level, and MRSA and MSSA nasal colonization.MethodsAll data used for this analysis came from the 2001–2004 National Health and Nutrition Examination Survey (NHANES). The analytical sample consisted of 18,626 participants aged 1 year and older. Multivariate logistic regression, including adjustment for demographic and dietary factors, was used to analyze the association between blood Pb and Cd, and nasal colonization by MRSA and MSSA.ResultsPrevalence of MRSA and MSSA carriage were 1.2%, and 29.3% respectively. MRSA was highest in women, individuals age 70 and older, who self-identified as black, had only a high school diploma, lived below 200% of the Federal Poverty Level, and had a history of smoking. While not significantly different from those colonized with MSSA, geometric mean blood Pb (1.74 μg/dL) and blood Cd (0.31 μg/L) were highest in those colonized with MRSA. Associations with MRSA colonization appeared to increase in a dose-dependent manner with increasing quartile of blood Pb level. Blood Cd level in the fourth quartile was also significantly associated with lower odds of MRSA colonization. Both metals were associated with lower odds of MSSA colonization.ConclusionsBoth MRSA and MSSA results suggest that general population levels of blood Pb but not Cd are associated with differences in nasal carriage of S. aureus. While further research is needed, reduction in heavy metal exposures such as lead, concurrently with maintaining a healthy microbiota may be two modifiable options to consider in the fight against antibiotic-resistance.

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Exposure of juvenile Leghorn chickens to lead acetate enhances antibiotic resistance in enteric bacterial flora

Cited by 13 publications

References 22 publications

Metagenomic Analysis Suggests Modern Freshwater Microbialites Harbor a Distinct Core Microbial Community

Metagenomic Analysis Suggests Modern Freshwater Microbialites Harbor a Distinct Core Microbial Community

Relationship between antibiotic resistance genes and metals in residential soil samples from Western Australia

Heavy metal exposure and nasal Staphylococcus aureus colonization: analysis of the National Health and Nutrition Examination Survey (NHANES)

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