Exposure of human skin to benzo[a]pyrene: Role of CYP1A1 and aryl hydrocarbon receptor in oxidative stress generation

Costa, Chiara; Catania, Stefania; Pasquale, R. De; Stancanelli, Rosanna; Scribano, G.M.; Melchini, Antonietta

doi:10.1016/j.tox.2010.02.014

Cited by 87 publications

(67 citation statements)

References 26 publications

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“…4a). Data obtained from western blotting analysis confirmed the presence of detectable amounts of both receptor and enzyme in basal conditions as previously reported (Costa et al 2010). Our results showed an increase in AhR and CYP1A1 protein expression by HE/HP-β-CyD only at the highest concentration (50 μM, p<0.001; Fig.…”

Section: Modulation Of Bap-induced Ahr and Cyp1a1 Protein Expression supporting

confidence: 91%

“…As the study required that skin maintained its metabolic competence, we adopted for sample preparation the method already described by Costa et al (2010). Human skin from healthy donors was obtained from cosmetic reduction surgery of the abdomen and kept cooled during transportation to the laboratory, which occurred within 2 h. Samples were visually examined in order to ascertain their integrity.…”

Section: Sample Preparation and Treatment With He/hp-β-cyd And Bapmentioning

confidence: 99%

“…BaP is a specific aryl hydrocarbon receptor (AhR) agonist able to modify the expression of several target genes including CYP 1A1 (Nakatsuru et al 2004). The inducible CYP 1A1, which has been individuated also in human and rodent skin, forms activated metabolites of BaP that can directly damage DNA and induce tumorigenesis (Costa et al 2010;Janmohamed et al 2001;Reiners et al 1998). Both AhR activation and CYP 1A1 induction are also responsible to enhance oxidative stress conditions increasing the physiological generation of reactive oxygen species (ROS) (Elbekai et al 2004;Stohs 1990).…”

Section: Introductionmentioning

confidence: 99%

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Interaction of a functionalized complex of the flavonoid hesperetin with the AhR pathway and CYP1A1 expression: involvement in its protective effects against benzo[a]pyrene-induced oxidative stress in human skin

et al. 2011

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Skin cancer pathogenesis is partially associated to the oxidative stress conditions induced by environmentally carcinogens such as benzo[a]pyrene (BaP). The protective effects against BaP-induced oxidative stress of the flavonoid hesperetin as a complex with the 2-hydroxypropyl-β-cyclodextrin (HE/HP-β-CyD) have been evaluated using an ex vivo human skin model. Human healthy skin has been pre-treated with the functionalized complex HE/HP-β-CyD (0.5-50 μM) before BaP (5 μM) application simulating occupational and environmental exposure. Oxidative stress was evaluated in terms of 3-(4, 5-dimethylthiazol-2-yl)-2, 5-dipheyltetrazolium bromide reduction, protein peroxidation and reactive oxygen species (ROS) formation. Additionally, it has been investigated whether the potential protective effects of HE/HP-β-CyD may be correlated to the interaction with aryl hydrocarbon receptor (AhR) pathway. A significant protection by HE/HP-β-CyD against the BaP-induced increase in ROS and carbonyl compound production, as well as reduction in tissue viability, has been observed (p<0.001). Results obtained showed that HE/HP-β-CyD was also able to reduce BaP-induced AhR and CYP1A1 protein expression (p<0.001). Experimental evidences provided from this study suggest significant preventive properties of HE/HP-β-CyD in the toxicity caused by environmental carcinogens such as PAHs.

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Section: Modulation Of Bap-induced Ahr and Cyp1a1 Protein Expression supporting

confidence: 91%

Section: Sample Preparation and Treatment With He/hp-β-cyd And Bapmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Interaction of a functionalized complex of the flavonoid hesperetin with the AhR pathway and CYP1A1 expression: involvement in its protective effects against benzo[a]pyrene-induced oxidative stress in human skin

et al. 2011

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“…UV exposure enhances ROS/RNS production [4,5,6], and the free radicals cause skin damage including apoptosis by interacting with DNAs, RNAs, and proteins [7]. Several lines of evidence have pointed to the implication of mitogen-activated protein kinase, aryl hydrocarbon receptor, transcription factors, such as nuclear factor-κB and nuclear factor erythroid 2-related factor 2, or matrix metalloproteinase in the degradation of dermal connective tissue following oxidative stress-induced skin damage [8,9,10,11,12,13,14,15]. …”

Section: Introductionmentioning

confidence: 99%

DCP-LA Exerts an Antiaging Action on the Skin

Nishizaki¹

2016

Skin Pharmacol Physiol

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The present study assessed the possibility for the linoleic acid derivative 8-[2-(2-pentyl-cyclopropylmethyl)-cyclopropyl]-octanoic acid (DCP-LA) as an antiaging compound for the skin by assaying senescence-associated β-galactosidase (SA-β-Gal), a biomarker of senescence and cell viability. The nitric oxide (NO) donor sodium nitroprusside (SNP) increased in SA-β-Gal-positive cells in cultured human fibroblasts and mouse keratinocytes, and DCP-LA significantly inhibited the effect of SNP. Moreover, SNP induced cell death in cultured mouse keratinocytes, and DCP-LA significantly prevented NO stress-induced death of keratinocytes. Taken together, these results indicate that DCP-LA exerts an antiaging action on the skin.

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“…Both the individual toxicity and human health risk assessment of these metals have been extensively reviewed and reported by various international regulatory agencies such as WHO and US EPA. Oxidative stress is attributed as the unifying factor for metal toxicity (Flora et al 2008, Henkler et al 2010 and metal induced oxidative stress may result in lipid peroxidation, damage to cellular protein and nucleic acids leading to a variety of cellular dysfunctions, including cell death (Valko et al 2005 (Costa et al 2010, Tsuji et al 2011. Naphthalene (Nap) (Group 2B carcinogen) is associated with hemolytic anemia, cataract and respiratory disorders (Stohs et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays

Muthusamy¹

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Mixed contamination of polyaromatic hydrocarbons (PAHs) and metals are ubiquitous in the environment. Some of the individual PAHs are known as human carcinogens, and metals are associated with various systemic toxicity and cancers in humans. Although the individual toxicity of PAHs and metals are well documented, our present state of knowledge about the adverse health effects of these mixtures is very limited in terms of exposure associated toxicity in humans, the underlying mechanism of toxicity and predicting the toxicity of mixtures. Due to this, their risk assessment has been mostly carried out based on their individual toxicity data.This research work investigated the interaction and combined toxicity of four PAHs

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Exposure of human skin to benzo[a]pyrene: Role of CYP1A1 and aryl hydrocarbon receptor in oxidative stress generation

Cited by 87 publications

References 26 publications

Interaction of a functionalized complex of the flavonoid hesperetin with the AhR pathway and CYP1A1 expression: involvement in its protective effects against benzo[a]pyrene-induced oxidative stress in human skin

Interaction of a functionalized complex of the flavonoid hesperetin with the AhR pathway and CYP1A1 expression: involvement in its protective effects against benzo[a]pyrene-induced oxidative stress in human skin

DCP-LA Exerts an Antiaging Action on the Skin

Evaluation of interaction and combined toxicity of polyaromatic hydrocarbons (PAHs) and metals using human cell-based bioassays

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