Exposure of fertilized porcine ova to pseudorabies virus

Bolin, Steve; Runnels, L J; Sawyer, C.A.; Atcheson, K.J.; Gustafson, D. P.

doi:10.1016/s0093-691x(79)80022-9

Cited by 3 publications

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“…In recent years, worldwide epidemics of infectious disease have become a problem in the swine industry, and the risk of transmission between farms increases when live pigs are introduced from outside. However, it has been shown that these risks can be reduced by using embryo transfer technology (Bolin et al, 1981;Wrathall, 1995), and more recently the development of practical non-surgical embryo transfer methods in pigs has made it possible to produce piglets with a high probability (Martinez et al, 2004(Martinez et al, , 2016Misumi et al, 2020). Embryo transfer technology is now becoming more widely applicable, allowing its use on farms (Martinez et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Induction of short‐term pseudopregnancy in gilts by the administration of estradiol benzoate or estradiol dipropionate to achieve ovulatory synchronization for embryo collection

Hirayama

Misumi

Yoshioka

et al. 2021

Animal Science Journal

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A study was conducted to investigate whether ovulation in gilts could be synchronized for embryo collection by the administration of estradiol benzoate (EB) or estradiol dipropionate (EDP) to induce pseudopregnancy, followed by the treatment with prostaglandin F 2α (PGF 2α ) on 10 days after. Ten gilts each received a total of 20 mg of EB or EDP on Day 10 or EB on Day 10 and 14 to induce pseudopregnancy (Day 0 = onset of estrus). Donors received PGF 2α 10 or 15 days (as a control) after the first administration of estrogens and subsequently eCG and hCG, and were then inseminated artificially. The embryos were collected 7 days after the administration of hCG, and assessed for embryo yield and their developmental stages. All protocols resulted in good embryo yield (9.8-13.2 embryos in average), and the embryos showed average ability to develop to the expanded blastocyst stage (3.29-4.03 as developmental scores) without any significant differences among the protocols. These results suggest that the administration of PGF 2α 10 days after the treatment of gilts with EB or EDP would allow synchronization of ovulation and embryo collection, as well as shortening the period from estrus detection to embryo collection, thus improving embryo collection efficiency.

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Section: Introductionmentioning

confidence: 99%

Induction of short‐term pseudopregnancy in gilts by the administration of estradiol benzoate or estradiol dipropionate to achieve ovulatory synchronization for embryo collection

Hirayama

Misumi

Yoshioka

et al. 2021

Animal Science Journal

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Embryo Transfer in Pigs

Gordon

1983

Controlled Breeding in Farm Animals

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Hygienic Applications of Biotechnical Measures in Pigs*

Hare¹

1991

Reprod Domestic Animals

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Contents: A key factor in the hygienic application of embryo transfer (ET) and its associated technologies is effective risk management. This depends on knowing what the risks of infectious disease transmission are and how they can be reduced or removed. Risk assessment depends on a knowledge of the pathogenesis of the disease, the procedures used in ET and its associated technologies and the results from research into infectious disease transmission by embryos or through ET. Two approaches can be taken to ensure the safe health status of the embryo: 1) by determining that the donor animals (male and female) are free from specific diseases, or 2) by ensuring that the zona pellucida (ZP)‐intact embryo is collected in a sanitary manner and handled (washed, treated, evaluated, etc.) according to recommended procedures. The former approach is applicable to diseases on which no or insufficient research has been done to determine the risk of their being transmitted by embryos or where the results of research done indicate a risk of the disease being transmitted. The latter approach is a preferred option with diseases where results of research done indicate that the risk of their being transmitted by embryos collected from infected or recovered donors is negligible. Infectious diseases for which research results indicate negligible risk of transmission by ZP‐intact (ZP‐I) embryos collected from infected or recovered donors include: pseudorabies (Aujesrky's disease), hog cholera (swine fever), foot and mouth disease, and swine vesicular disease. Infectious diseases on which insufficient research has been done include: African swine fever, vesicular stomatitis, enterovirus disease, parvouirus disease and leptospirosis. A problem associated with this approach is ensuring that procedures shown to be effective under experimental conditions are properly carried out under field conditions: the use of nationally accredited embryo collection teams may solve this problem. Healthy recipients and good record keeping are also critical factors in the hygienic application of ET. The health status of an embryo collected from a specific disease‐free donor will not be adversely affected by damage to its ZP. Where research has shown that ZP‐I embryos that have been exposed to particular pathogens are not infected or contaminated after proper washing or washing and treatment, it can be assumed that the risks of transmission of the diseases caused by these pathogens will not be increased by deliberate (micromanipulation) or accidental damage to the ZP after the washing/treatment procedures have been carried out. The risks of infectious disease transmission when embryos are produced by in vitro fertilization or blastomere transplantation to mature oocytes, followed by culture, have yet to be determined.

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Exposure of fertilized porcine ova to pseudorabies virus

Cited by 3 publications

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Induction of short‐term pseudopregnancy in gilts by the administration of estradiol benzoate or estradiol dipropionate to achieve ovulatory synchronization for embryo collection

Induction of short‐term pseudopregnancy in gilts by the administration of estradiol benzoate or estradiol dipropionate to achieve ovulatory synchronization for embryo collection

Embryo Transfer in Pigs

Hygienic Applications of Biotechnical Measures in Pigs*

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