This paper investigates the relative effect of material properties and structural details in the joint panels on seismic fragility of existing reinforced concrete (RC) frames. Five building classes with different structural details (particularly in the joint panels) and material characteristics are defined according to different past design codes, for a three-and a six-storey archetype geometry. Based on non-linear static or non-linear dynamic analysis procedures, results from the study show that the effect of structural details on seismic fragility of the considered structures is negligible for damage states involving an essentially-elastic behaviour. Conversely, this is much higher for the life-safety and near-collapse damage states, and it is considerably higher than the one due to materials. Therefore, in the diagnosis phase, higher emphasis should be given to on-site investigations on the actual reinforcement content/layout rather than to invasive material testing. The uncertainty related to the structural details described herein is practically related to the exterior, rather than interior, joint panels. Cover removal for one of those joints could potentially eliminate this specific uncertainty. As a practical action, a good practice for the in-situ testing of RC frames