Exposure and risk assessment of acetamiprid in honey bee colonies under a real exposure scenario in Eucalyptus sp. landscapes

Capela, Nuno; Xu, Mang; Simões, Sandra; Azevedo-Pereira, Henrique M.V.S.; Peters, Jeroen; Sousa, José Paulo

doi:10.1016/j.scitotenv.2022.156485

Cited by 10 publications

(7 citation statements)

References 64 publications

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“…For instance, Capela et al. (2022) reported that acetamiprid residue concentrations ranged from 11 to 1000 ppb on the day after application in a Eucalyptus sp. plantation, while Pohorecka et al.…”

Section: Discussionmentioning

confidence: 99%

Chronic acetamiprid exposure moderately affects the foraging behaviour of buff‐tailed bumblebees (Bombus terrestris)

Tóth,

Kovács

2023

Ethology

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Bumblebees are important pollinators in both natural and agricultural ecosystems, but their survival and pollination service are threatened by extensive pesticide use. Due to regulation changes, acetamiprid has become the only neonicotinoid substance that can be used without restrictions and in open‐field cultivations in the European Union. Yet, we know little about how this active ingredient affects bumblebees' foraging behaviour and if such effects are similarly detrimental to those of other neonicotinoids. Here we investigated how a 14‐day‐long (chronic) exposure to low (5 ppb) and high (2500 ppb) concentrations of acetamiprid in syrup affected different aspects of foraging behaviour in buff‐tailed bumblebees (Bombus terrestris, Linnaeus). We recorded individual foraging at artificial food patches during one‐hour‐long trials and then compared several foraging‐related measures between differently dosed individuals. We found that 75.12% of the total syrup consumption occurred at the first‐exploited patch, but individuals did not exhibit any bias toward pesticide‐treated food patches. Chronic exposure to acetamiprid had little effect on individual foraging decisions at the first‐exploited food patch but significantly affected the time to the first feeding and the number of feedings at the second‐exploited patch in interaction with body mass. The duration of the first feeding was affected only by foragers' body mass. Our finding indicates that chronic exposure to a high but field‐realistic concentration of acetamiprid may alter some aspects of bumblebees' foraging behaviour. If such behavioural changes accumulate during consecutive foraging bouts, chronic exposure to this pesticide might lead to a reduction of daily resource collection, ultimately jeopardising colony fitness or plant (crop) pollination.

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Section: Discussionmentioning

confidence: 99%

Chronic acetamiprid exposure moderately affects the foraging behaviour of buff‐tailed bumblebees (Bombus terrestris)

Tóth,

Kovács

2023

Ethology

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“…This was already successfully proven by the implementation of the Ace assay for screening a wide range of matrices in Eucalyptus sp. plantations [ 52 ]. Moreover, their implementation by plant retailers allows for stricter control in the “grower to customer” chain as both of them desire an effective phasing out of unwanted pesticides like neonicotinoids for more sustainable food and product chains.…”

Section: Discussionmentioning

confidence: 99%

Neonicotinoid Microsphere Immunosensing for Profiling Applications in Honeybees and Bee-Related Matrices

Portier

Bovee

et al. 2022

Biosensors

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Neonicotinoids are the most commonly used insecticides due to their effectiveness. However, non-targeted insects, especially bees, are also affected by neonicotinoids. Therefore, neonicotinoid application can contribute to the declining bee populations worldwide. The presented study describes the development of novel competitive, fluorescent microsphere-based suspension immunoassays for neonicotinoid profiling and their application to bees and essential bee-related matrices, using the Multi-Analyte Profiling (xMAP) technology. For the construction of these neonicotinoid microsphere immunoassays (nMIAs), neonicotinoid–ovalbumin conjugates were coupled to unique fluorescent, paramagnetic microspheres, which competed with the free neonicotinoids that were present in test samples for interacting with the corresponding, specific antibodies. In total, five independent nMIA’s were developed for the detection of imidacloprid, acetamiprid, clothianidin, thiacloprid, thiamethoxam, dinotefuran, nitenpyram and imidaclothiz with the limits of detection being for 0.01 ng/mL, 0.01 ng/mL, 0.02 ng/mL, 0.02 ng/mL, 0.003 ng/mL, 2.95 ng/mL, 0.09 ng/mL and 0.04 ng/mL, respectively. The developed nMIAs were applied to fortified matrices including surface water, pollen, honey and honeybees. All of the neonicotinoids, except dinotefuran, could be sensitively detected in all of the tested environmental matrices and bees, with there being sensitivities of 1 ng/mL in water and 10 ng/g in solid materials. These nMIAs provide a rapid profiling method for all of the common neonicotinoids, including those that are banned by the European Union for outdoor use. The developed method can contribute to healthy and sustainable beekeeping, globally, via its application in the apiary environment.

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“…This substance is reported to be non-toxic to pollinators due to its rapid metabolisation 11 , yet recent investigations suggest that in high doses, it can induce various sub-lethal effects and thus pose a threat to pollinators 12 , 13 , natural predators of pests 14 , 15 and other non-target organisms including vertebrates 16 . Previous investigations indicate that the field-relevant concentrations of acetamiprid can vary widely with specific environmental conditions and locations 17 , 18 , and even the concentrations of residues in flowers following application may range within several orders of magnitude (from 0.1 to 3360 ppb 19 – 21 ), probably depending on the mode of application and crop type. Due to the apparent uncertainty associated with the exposure levels to this substance (including the exposure of humans to this neonicotinoid 22 ), the sub-lethal effects of acetamiprid applied in agricultural production are likely to remain in the focus of ecotoxicology, conservation biology and health science research in the coming years.…”

Section: Introductionmentioning

confidence: 99%

“…in flowers following application may range within several orders of magnitude (from 0.1 to 3360 ppb [19][20][21] ), probably depending on the mode of application and crop type. Due to the apparent uncertainty associated with the exposure levels to this substance (including the exposure of humans to this neonicotinoid 22 ), the sub-lethal effects of acetamiprid applied in agricultural production are likely to remain in the focus of ecotoxicology, conservation biology and health science research in the coming years.…”

mentioning

confidence: 99%

Exposure to an insecticide formulation alters chemosensory orientation, but not floral scent detection, in buff-tailed bumblebees (Bombus terrestris)

Kárpáti,

Szelényi,

Tóth

2024

Sci Rep

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Although pesticide-free techniques have been developed in agriculture, pesticides are still routinely used against weeds, pests, and pathogens worldwide. These agrochemicals pollute the environment and can negatively impact human health, biodiversity and ecosystem services. Acetamiprid, an approved neonicotinoid pesticide in the EU, may exert sub-lethal effects on pollinators and other organisms. However, our knowledge on the scope and severity of such effects is still incomplete. Our experiments focused on the effects of the insecticide formulation Mospilan (active ingredient: 20% acetamiprid) on the peripheral olfactory detection of a synthetic floral blend and foraging behaviour of buff-tailed bumblebee (Bombus terrestris) workers. We found that the applied treatment did not affect the antennal detection of the floral blend; however, it induced alterations in their foraging behaviour. Pesticide-treated individuals started foraging later, and the probability of finding the floral blend was lower than that of the control bumblebees. However, exposed bumblebees found the scent source faster than the controls. These results suggest that acetamiprid-containing Mospilan may disrupt the activity and orientation of foraging bumblebees. We hypothesize that the observed effects of pesticide exposure on foraging behaviour could be mediated through neurophysiological and endocrine mechanisms. We propose that future investigations should clarify whether such sub-lethal effects can affect pollinators’ population dynamics and their ecosystem services.

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Exposure and risk assessment of acetamiprid in honey bee colonies under a real exposure scenario in Eucalyptus sp. landscapes

Cited by 10 publications

References 64 publications

Chronic acetamiprid exposure moderately affects the foraging behaviour of buff‐tailed bumblebees (Bombus terrestris)

Chronic acetamiprid exposure moderately affects the foraging behaviour of buff‐tailed bumblebees (Bombus terrestris)

Neonicotinoid Microsphere Immunosensing for Profiling Applications in Honeybees and Bee-Related Matrices

Exposure to an insecticide formulation alters chemosensory orientation, but not floral scent detection, in buff-tailed bumblebees (Bombus terrestris)

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