Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution

Hu, Zhiqiang; Zhang, Xuehong; Wu, Zhenqiang; Qi, Haifeng; Wang, Zhilong

doi:10.1016/j.jbiotec.2012.10.004

Cited by 57 publications

(46 citation statements)

References 41 publications

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“…High extracellular pigment concentration was also achieved by perstractive fermentation using two‐stage operation mode, i.e. cultivation of microbes in an aqueous solution for microbial growth in the first stage and then perstractive fermentation in a nonionic surfactant micelle aqueous solution for production of extracellular pigments in the second stage (Hu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Solubilization capacity of nonionic surfactant micelles exhibiting strong influence on export of intracellular pigments in Monascus fermentation

Kang¹,

Zhang

et al. 2013

Microbial Biotechnology

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SummaryIn this study, perstractive fermentation of intracellular Monascus pigments in nonionic surfactant micelle aqueous solution had been studied. The permeability of cell membrane modified by nonionic surfactant might have influence on the rate of export of intracellular pigments into its extracellular broth while nearly no effect on the final extracellular pigment concentration. However, the solubilization of pigments in nonionic surfactant micelles strongly affected the final extracellular pigment concentration. The solubilization capacity of micelles depended on the kind of nonionic surfactant, the super-molecule assembly structure of nonionic surfactant in an aqueous solution, and the nonionic surfactant concentration. Elimination of pigment degradation by export of intracellular Monascus pigments and solubilizing them into nonionic surfactant micelles was also confirmed experimentally. Thus, nonionic surfactant micelle aqueous solution is potential for replacement of organic solvent for perstractive fermentation of intracellular product.

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Section: Introductionmentioning

confidence: 99%

Solubilization capacity of nonionic surfactant micelles exhibiting strong influence on export of intracellular pigments in Monascus fermentation

Kang¹,

Zhang

et al. 2013

Microbial Biotechnology

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“…These features play a very important role to achieve the desired yields of a target metabolite [121]. The configuration and volume of the bioreactor is an important factor to consider in the microbial production of carotenoids.…”

Section: Introductionmentioning

confidence: 99%

Biotechnological production of carotenoids by yeasts: an overview

et al. 2014

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Nowadays, carotenoids are valuable molecules in different industries such as chemical, pharmaceutical, poultry, food and cosmetics. These pigments not only can act as vitamin A precursors, but also they have coloring and antioxidant properties, which have attracted the attention of the industries and researchers. The carotenoid production through chemical synthesis or extraction from plants is limited by low yields that results in high production costs. This leads to research of microbial production of carotenoids, as an alternative that has shown better yields than other aforementioned. In addition, the microbial production of carotenoids could be a better option about costs, looking for alternatives like the use of low-cost substrates as agro-industrials wastes. Yeasts have demonstrated to be carotenoid producer showing an important growing capacity in several agro-industrial wastes producing high levels of carotenoids. Agro-industrial wastes provide carbon and nitrogen source necessary, and others elements to carry out the microbial metabolism diminishing the production costs and avoiding pollution from these agro-industrial wastes to the environmental. Herein, we discuss the general and applied concepts regarding yeasts carotenoid production and the factors influencing carotenogenesis using agro-industrial wastes as low-cost substrates.

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“…Compared with other nonionic surfactants such as Tween 80, Span 20, Triton X-114, Brij 30, PEG 4000, and Pluronic, Triton X-100 (TX) showed an excellent biocompatibility to fungal growth and extractive efficiency for Monascus pigment in an extractive fermentation (Hu et al 2012a, b). By using TX as the additive, an enrichment of the hydrophobic pigments occurred upon being “milked” in the artificial nonionic micelle aqueous solution to improve the pigment penetration across the cellular membrane (Hu et al 2012b; Kang et al 2013a). Further study indicated that the nonionic surfactant may modify the cell membrane lipid layer and then improve the rate of secretion of intracellular hydrophobic pigments across the cell membrane (Wang et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution

et al. 2017

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Extractive fermentation in a nonionic surfactant aqueous solution provides a promising and efficient method to produce Monascus pigments. The behaviour of pigment secretion during the extractive cultivation was investigated in the present work. The results revealed that the secretion of intracellular pigment was limited by its saturation concentration in the nonionic surfactant aqueous solution. The intracellular pigment was completely extracted to the outside of the cell at a low cell density and high concentration of Triton X-100 (TX) in fermentation broth; otherwise, a restriction for pigment extraction would occur. The decrement of the intracellular orange and yellow pigments was inconsistent with the increment of extracellular pigments with an increase in the TX concentration. It could be inferred that the intracellular orange pigment was converted to extracellular yellow pigment during the transmembrane secretion process, which might be attributed to the enzyme catalysis in the non-aqueous phase solution. This study helps explain the mechanism of variation of pigment characteristic and extraction capacity in extractive fermentation.

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Export of intracellular Monascus pigments by two-stage microbial fermentation in nonionic surfactant micelle aqueous solution

Cited by 57 publications

References 41 publications

Solubilization capacity of nonionic surfactant micelles exhibiting strong influence on export of intracellular pigments in Monascus fermentation

Solubilization capacity of nonionic surfactant micelles exhibiting strong influence on export of intracellular pigments in Monascus fermentation

Biotechnological production of carotenoids by yeasts: an overview

Saturation effect and transmembrane conversion of Monascus pigment in nonionic surfactant aqueous solution

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