Exponential amplification by redox cross-catalysis and unmasking of doubly protected molecular probes

Pallu, Justine; Rabin, Charlie; Pan, Hui; Moreira, Thamires S; Creste, Geordie; Calvet, Corentin; Limoges, Benoı̂t; Mavré, François; Branca, Mathieu

doi:10.1039/d1sc06086d

Cited by 1 publication

(1 citation statement)

References 61 publications

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“…Particularly, the characteristic sigmoidal reaction/transduction profile of autocatalysis reaction facilitates the most effective signal amplification. − Among these artificial molecular circuits, nucleic acid-based autocatalytic circuits have been designed to detect various biomarkers due to their high programmability, accurate recognition capability, fine biocompatibility, and desirable controllability. − However, the existing autocatalytic systems are constrained to the following aspects. First, autocatalytic systems are complicated because they usually involve more different reactants, thus leading to undesired side reactions and inevitable signal leakage, which affect their sensitivity and specificity. , Second, some autocatalytic circuits require the assistance of enzymes, which need strict operating conditions and are thus costly.…”

Section: Introductionmentioning

confidence: 99%

Self-Stacking Autocatalytic Molecular Circuit with Minimal Catalytic DNA Assembly

Zhu

Gong

et al. 2023

J. Am. Chem. Soc.

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Isothermal autocatalytic DNA circuits have been proven to be versatile and powerful biocomputing platforms by virtue of their self-sustainable and self-accelerating reaction profiles, yet they are currently constrained by their complicated designs, severe signal leakages, and unclear reaction mechanisms. Herein, we developed a simpler-yet-efficient autocatalytic assembly circuit (AAC) for highly robust bioimaging in live cells and mice. The scalable and sustainable AAC system was composed of a mere catalytic DNA assembly reaction with minimal strand complexity and, upon specific stimulation, could reproduce numerous new triggers to expedite the whole reaction. Through in-depth theoretical simulations and systematic experimental demonstrations, the catalytic efficiency of these reproduced triggers was found to play a vital role in the autocatalytic profile and thus could be facilely improved to achieve more efficient and characteristic autocatalytic signal amplification. Due to its exponentially high signal amplification and minimal reaction components, our self-stacking AAC facilitated the efficient detection of trace biomolecules with low signal leakage, thus providing great clinical diagnosis and therapeutic assessment potential.

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Section: Introductionmentioning

confidence: 99%

Self-Stacking Autocatalytic Molecular Circuit with Minimal Catalytic DNA Assembly

Zhu

Gong

et al. 2023

J. Am. Chem. Soc.

View full text Add to dashboard Cite

show abstract

Exponential amplification by redox cross-catalysis and unmasking of doubly protected molecular probes

Abstract: The strength of autocatalytic reactions lies in their ability to provide a powerful means of molecular amplification, which can be very useful for improving the analytical performances of a multitude...

Cited by 1 publication

References 61 publications

Self-Stacking Autocatalytic Molecular Circuit with Minimal Catalytic DNA Assembly

Self-Stacking Autocatalytic Molecular Circuit with Minimal Catalytic DNA Assembly

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