Exploring winter diet, gut microbiota and parasitism in caribou using multi-marker metabarcoding of fecal DNA
Mélissande Nagati,
Marie-Josée Bergeron,
Patrick Gagné
et al.
Abstract:In conservation strategies, getting precise and repeatable information on the species’ diet and health without relying on invasive or laborious methods is challenging. Here, we developed an efficient and non-invasive workflow for the sequencing and analysis of four taxonomic markers from fecal DNA to characterize the gut microbiota, parasites, and plants and lichens composing the winter diet of caribou (Rangifer tarandus), Canada's most iconic endangered species. Sequencing of the 18S rRNA gene of eukaryotes f… Show more
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