Exploring Vaccinium vitis-idaea L. as a potential source of therapeutic agents: antimicrobial, antioxidant, and anti-inflammatory activities of extracts and fractions

Vilkickytė, Gabrielė; Petrikaitė, Vilma; Pukalskas, Audrius; Šipailienė, Aušra; Raudonė, Lina

doi:10.1016/j.jep.2022.115207

Cited by 9 publications

(5 citation statements)

References 79 publications

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“…One primary problem with the analysis of proanthocyanidins is that due to the complexities of the structures and different linkages, the results of analytical procedures can often be erroneous, non-reproducible, non-selective, or not quantifiable [ 30 ]. Previously, PRF was additionally analyzed by our research group using the UPLC-PDA/ESI-QTOF-MS method to confirm the identity of proanthocyanidins and suggest more A-type procyanidin trimers [ 31 ]. Other studies indicated that oligomeric (mDP 4–10) and polymeric (mDP > 10) proanthocyanidins can be found in the lingonberry matrix as well [ 2 , 32 ], thus suggesting unidentified larger polymeric molecules in our tested sample.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, the main effects of plant secondary metabolites working toward wound healing can be addressed as cell migration and proliferation as well as antimicrobial, anti-inflammatory, and antioxidant activities [ 17 ]. Previously, our group reported that PRF from lingonberry leaves surpassed all other phenolic fractions and crude extracts by the highest antioxidant, anti-inflammatory potential, and strongest antimicrobial properties [ 31 ]. In addition, cytotoxic PRF activity has been established in a previous experiment against human colon adenocarcinoma HT-29, renal carcinoma CaKi-1, and melanoma IGR39 cell lines, and was in the range from 30 to 50 µg/mL [ 29 ].…”

Section: Discussionmentioning

confidence: 99%

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Proanthocyanidins from Vaccinium vitis-idaea L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery

Vilkickytė

Žilius

Petrikaitė

et al. 2022

Plants

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The compositions and health-beneficial properties of lingonberry leaves (Vaccinium vitis-idaea L.) are well established; however, their proanthocyanidins are still heavily underutilized. Optimizing their delivery systems is key to enabling their wider applications. The present study investigates the phytochemical and ‘wound-healing’ properties of proanthocyanidin-rich fraction(s) (PRF) from lingonberry leaves as well as the development of optimal dermal film as a proanthocyanidin delivery system. The obtained PRF was subjected to HPLC-PDA and DMAC analyses to confirm the qualitative and quantitative profiles of different polymerization-degree proanthocyanidins. A ‘wound healing’ in vitro assay was performed to assess the ability of PRF to modulate the wound environment for better healing. Low concentrations of lingonberry proanthocyanidins were found to accelerate ‘wound‘ closures, while high levels inhibited human fibroblast migration. Fifteen dermal films containing PRF were prepared and evaluated based on their polymer (MC, HEC, PEG 400) compositions, and physical, mechanical, and biopharmaceutical properties using an experimental design. The composition containing 0.30 g of MC, 0.05 g of HEC, and 3.0 g of PEG 400 was selected as a promising formulation for PRF delivery and a potentially effective functional wound dressing material, supporting the need for further investigations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Proanthocyanidins from Vaccinium vitis-idaea L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery

Vilkickytė

Žilius

Petrikaitė

et al. 2022

Plants

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“…However, the UAE extract presented a bactericidal effect on K. pneumoniae and P. aeruginosa while the CE extract presented a bactericidal effect only on P. aeruginosa. Recently, Vilkickyte et al [60] reported that an extract from lingonberry leaves showed no antimicrobial activity against E. coli. Moreover, Tian et al [61] reported that E. coli presented low sensitivity to an extract of berry plants and no inhibitory effect was noticed in extracts derived from bilberry, chokeberry and nettle leaves.…”

Section: Antimicrobial Activity Of Bog Bilberry Leaf Extractsmentioning

confidence: 99%

Characterization of the Chemical Composition and Biological Activities of Bog Bilberry (Vaccinium uliginosum L.) Leaf Extracts Obtained via Various Extraction Techniques

Ștefănescu,

Socaci,

Fărcaș

et al. 2024

Foods

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This investigation aimed to assess the chemical composition and biological activities of bog bilberry (Vaccinium uliginosum L.) leaves. Hydroethanolic extracts were obtained using four extraction techniques: one conventional (CE) and three alternative methods; ultrasound (UAE), microwave (MAE) and high-pressure (HPE) extractions. Spectrophotometric analysis was conducted to determine their chemical content, including the total phenolic content (TPC) and total flavonoid content (TFC). Furthermore, their antioxidative and antimicrobial properties were evaluated. HPLC (high performance liquid chromatography) analysis identified and quantified 17 phenolic compounds, with chlorogenic acid being the predominant compound, with the lowest level (37.36 ± 0.06 mg/g) for the bog bilberry leaf extract obtained by CE and the highest levels (e.g., HPE = 44.47 ± 0.08 mg/g) for the bog bilberry leaf extracts obtained by the alternative methods. Extracts obtained by HPE, UAE and MAE presented TPC values (135.75 ± 2.86 mg GAE/g; 130.52 ± 1.99 mg GAE/g; 119.23 ± 1.79 mg GAE/g) higher than those obtained by the CE method (113.07 ± 0.98 mg GAE/g). Regarding the TFC values, similar to TPC, the highest levels were registered in the extracts obtained by alternative methods (HPE = 43.16 ± 0.12 mg QE/g; MAE = 39.79 ± 0.41 mg QE/g and UAE = 33.89 ± 0.35 mg QE/g), while the CE extract registered the lowest level, 31.47 ± 0.28 mg QE/g. In the case of DPPH (1,1-diphenyl-2-picrylhydrazyl) antioxidant activity, the extracts from HPE, UAE and MAE exhibited the strongest radical scavenging capacities of 71.14%, 63.13% and 60.84%, respectively, whereas the CE extract registered only 55.37%. According to Microbiology Reader LogPhase 600 (BioTek), a common MIC value of 8.88 mg/mL was registered for all types of extracts against Staphylococcus aureus (Gram-positive bacteria) and Salmonella enterica (Gram-negative bacteria). Moreover, the alternative extraction methods (UAE, HPE) effectively inhibited the growth of Candida parapsilosis, in comparison to the lack of inhibition from the CE method. This study provides valuable insights into bog bilberry leaf extracts, reporting a comprehensive evaluation of their chemical composition and associated biological activities, with alternative extraction methods presenting greater potential for the recovery of phenolic compounds with increased biological activities than the conventional method.

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“…A search for the genus name Vaccinium or the combination Vaccinium + a drug in the archive of biomedical research articles in PubMed Central revealed 9868 and 3542 papers, respectively, with 56% of the studies published in the last 3 years. Such interest is primarily due to the growing demand for these plants as food and medicinal resources, effective in the prevention and treatment of many diseases [9][10][11][12], including COVID-19 [13,14].…”

Section: Introductionmentioning

confidence: 99%

Phosphorus Supply to Plants of Vaccinium L. Genus: Proven Patterns and Unexplored Issues

Struchkova,

Mikheev,

Berezina

et al. 2024

Agronomy

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Phosphorus availability is a serious problem for plants growing and grown in acidic soils of bogs, poor in macronutrients. The application of phosphorus fertilizers to such soils is unprofitable because of the physical and chemical properties of these soils, where phosphate is firmly bound to organic and inorganic compounds and becomes inaccessible to plants. Plants of the Vaccinium genus both from natural stands and commercial plantations may suffer from phosphorus deficiency, so they need to have a number of adaptations that allow them to efficiently extract phosphorus. This review addresses the following issues in relation to plants of the Vaccinium genus: sources of phosphorus for plants; the release of phosphate ions from soil components; the transport of phosphate ions into plants; and the importance of mycorrhiza in supplying phosphorus to plants. Thus, we sought to draw researchers’ attention to sources and routes of phosphorus supply of plants of the Vaccinium genus and its unexplored aspects.

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Exploring Vaccinium vitis-idaea L. as a potential source of therapeutic agents: antimicrobial, antioxidant, and anti-inflammatory activities of extracts and fractions

Cited by 9 publications

References 79 publications

Proanthocyanidins from Vaccinium vitis-idaea L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery

Proanthocyanidins from Vaccinium vitis-idaea L. Leaves: Perspectives in Wound Healing and Designing for Topical Delivery

Characterization of the Chemical Composition and Biological Activities of Bog Bilberry (Vaccinium uliginosum L.) Leaf Extracts Obtained via Various Extraction Techniques

Phosphorus Supply to Plants of Vaccinium L. Genus: Proven Patterns and Unexplored Issues

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