Exploring the sublethal genotoxic effects of class II organophosphorus insecticide quinalphos on freshwater fish Cyprinus carpio

Hemalatha, Devan; Nataraj, Bojan; Rangasamy, Basuvannan; Maharajan, Kannan; Ramesh, Mathan

doi:10.1007/s00343-019-9104-y

Cited by 8 publications

(5 citation statements)

References 67 publications

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“…After exposure of monocrotrophos and organophosphate to zebra fish Micronuleus test and comet assay indicated formation of micronulei and DNA damage (Dcosta et al, 2018). Similarly, many Authors (Naz et al, 2021;Hemalatha et al, 2020;Davico et al, 2020)…”

Section: Resultsmentioning

confidence: 99%

Chlorpyrifos and Endosulfan Mixture Induced DNA Damage and Nuclear Anomalies in RBCs of Labeo rohita Assessed Through Comet and Micronucleus Assay

Naz,

Abdullah,

Ahmed

et al. 2024

PUJZ

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Insecticides are widely used to control the insect damaging food and cash crops as well as the fruit plants. However, terrestrial and aquatic biodiversity is directly affected by overuse of insecticides. Present study was conducted to find out the genotoxic effect of insecticides on DNA damage and nuclear anomalies in RBCs of Labeo rohita after exposure to chlorpyrifos+endosulfan (CPF+END) by using Comet and Micronucleus Assay. Twenty fingerlings of L. rohita was kept in LC 50 concentration of CPF+END, (1.95±0.02 μgL -1 for 96 h) for 5 days after acclimatization. The blood sample of 5 fish was collected after 24, 48, 72 and 96 h to see the DNA damage and nuclear anomalies. Results of the current study revealed that damaged nuclei (DN%) and genetic damage index (GDI) in RBCs of L. rohita exposed to insecticides mixture was increased throughout the experiment. Similarly, the frequency of micronuclei (MN) and other nuclear anomalies (NAs) were also increased as the duration of exposure passed. It is concluded that exposure of fish to insecticides may cause considerable genetic damage to the fish. The both Comet and MN Assay are good biomarkers to identify the insecticides pollution. The results of our study can be utilized in environmental risk evaluation and in bio-monitoring approaches.Novelty Statement | This research sheds light on the genotoxicity and environmental risks associated with endosulfan and chlorpyrifos pesticides in freshwater ecosystems and offers new insights into the potential synergistic effects of these pesticides on aquatic organisms.

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Section: Resultsmentioning

confidence: 99%

Chlorpyrifos and Endosulfan Mixture Induced DNA Damage and Nuclear Anomalies in RBCs of Labeo rohita Assessed Through Comet and Micronucleus Assay

Naz,

Abdullah,

Ahmed

et al. 2024

PUJZ

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“…Moreover, it is possible that FLX affects aquatic animals such as microalgae, water fleas, plankton, and midges, including some fishes [52] . Fish are often used as bioindicators to determine the genotoxic and mutagenic effects of contaminants present in the aquatic environment [53] . In the past decade, there have been limited reports of FLX genotoxicity in fish.…”

Section: Discussionmentioning

confidence: 99%

“…For example, intracellular ROS production in haemocytes of Mytilus edulis was increased after exposure to FLX at concentrations of 10 and 50 mg/L, thus affecting haemocyte DNA damage [62] . Additionally, ROS can disrupt DNA replication and transcription [46] , as well as inhibit DNA repair [53] . A previous study found that Carassius auratus had increased malondialdehyde (MDA) content after exposure to FLX [63] .…”

Section: Discussionmentioning

confidence: 99%

Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure

et al. 2022

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“…Os peixes são considerados um modelo genético adequado para avaliação de alterações ambientais em ecossistemas aquáticos, devido a sua capacidade de metabolizar e acumular poluentes de forma eficiente (Hemalatha et al, 2021). Além de fornecer proteínas de alta qualidade, esses animais também são uma excelente fonte de nutrientes essenciais, como vitaminas A e D, selênio, iodo, ácido eicosapentaenóico e ácido docosahexaenóico (Chan et al, 2018).…”

Section: Introductionunclassified

Investigação de Alterações Genotóxicas em Hoplosternum littoralle, Frente a Contaminação Ambiental, com o Uso do Teste do Micronúcleo

Da Silva Soares,

Araújo da Silva

2023

J. Educ. Sci and Health

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O teste do micronúcleo (MN) é uma análise rápida e simples, que detecta a presença de danos genotóxicos no material genético de indivíduos expostos a agentes clastogênicos. Esse teste vem sendo utilizado para mensurar o grau de exposição aos contaminantes ambientais que causam efeitos genotóxicos. Esse estudo analisou a presença de alterações genotóxicas, utilizando o teste do micronúcleo, em células de peixe da espécie Hoplosternum littorale (tamoatá). Foram analisadas 20 amostras coletadas nos igarapés da Comunidade da Sharp, Presidente Dutra e Ponte da Paraíba, em Manaus, Amazonas. Esses ambientes são poluídos por efluentes que são lançados em suas águas. Foram analisadas 1000 células por lâmina (n = 20.000 células analisadas no total), no período de outubro a novembro de 2022, foram analisadas no laboratório multidisciplinar do Centro Universitário do Norte- UNN, na cidade de Manaus. Foram identificados 82 micronúcleos nas células da espécie amostrada dos três igarapés. Os indivíduos coletados no igarapé da Comunidade da Sharp, apresentaram um total de micronúcleos muito maior, correspondendo a 73,1 % do total desses danos, em comparação os indivíduos dos outros igarapés (Ponte Presidente Dutra 17,07% e Ponte da Paraíba 9,7%). A quantidade de micronúcleos é significativa, qualitativamente para indicativo de possíveis agentes causadores de danos genotóxicos nos peixes analisados, provenientes desses ambientes.

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Exploring the sublethal genotoxic effects of class II organophosphorus insecticide quinalphos on freshwater fish Cyprinus carpio

Cited by 8 publications

References 67 publications

Chlorpyrifos and Endosulfan Mixture Induced DNA Damage and Nuclear Anomalies in RBCs of Labeo rohita Assessed Through Comet and Micronucleus Assay

Chlorpyrifos and Endosulfan Mixture Induced DNA Damage and Nuclear Anomalies in RBCs of Labeo rohita Assessed Through Comet and Micronucleus Assay

Investigation of genotoxicity, mutagenicity, and cytotoxicity in erythrocytes of Nile tilapia (Oreochromis niloticus) after fluoxetine exposure

Investigação de Alterações Genotóxicas em Hoplosternum littoralle, Frente a Contaminação Ambiental, com o Uso do Teste do Micronúcleo

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