Exploring the Schistosoma mansoni adult male transcriptome using RNA-seq

Almeida, Giulliana T.; Amaral, Murilo Sena; Beckedorff, Felipe; Kitajima, João Paulo; DeMarco, Ricardo; Verjovski-Almeida, Sérgio

doi:10.1016/j.exppara.2011.06.010

Cited by 37 publications

(33 citation statements)

References 30 publications

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“…With second generation massively parallel sequencing platforms, transcriptome sequencing (RNA-Seq) has become widely used. In terms of parasites, the transcriptomes of Plasmodium falciparum [22], Trypanosoma brucei [23], Schistosoma mansoni [24], Trichuris suis [25], Dermanyssus gallinae [26] and others [27–29] have been sequenced. To better understand the intricacy of gene function and the activity of cells, description of the transcriptome is useful [30].…”

Section: Introductionmentioning

confidence: 99%

Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

et al. 2016

Parasites Vectors

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BackgroundPsoroptic mange is a chronic, refractory, contagious and infectious disease mainly caused by the mange mite Psoroptes ovis, which can infect horses, sheep, buffaloes, rabbits, other domestic animals, deer, wild camels, foxes, minks, lemurs, alpacas, elks and other wild animals. Features of the disease include intense pruritus and dermatitis, depilation and hyperkeratosis, which ultimately result in emaciation or death caused by secondary bacterial infections. The infestation is usually transmitted by close contact between animals. Psoroptic mange is widespread in the world. In this paper, the transcriptome of P. ovis is described following sequencing and analysis of transcripts from samples of larvae (i.e. the Pso_L group) and nymphs and adults (i.e. the Pso_N_A group). The study describes differentially expressed genes (DEGs) and genes encoding allergens, which help understanding the biology of P. ovis and lay foundations for the development of vaccine antigens and drug target screening.MethodsThe transcriptome of P. ovis was assembled and analyzed using bioinformatic tools. The unigenes of P. ovis from each developmental stage and the unigenes differentially between developmental stages were compared with allergen protein sequences contained in the allergen database website to predict potential allergens.ResultsWe identified 38,836 unigenes, whose mean length was 825 bp. On the basis of sequence similarity with seven databases, a total of 17,366 unigenes were annotated. A total of 1,316 DEGs were identified, including 496 upregulated and 820 downregulated in the Pso_L group compared with the Pso_N_A group. We predicted 205 allergens genes in the two developmental stages similar to genes from other mites and ticks, of these, 14 were among the upregulated DEGs and 26 among the downregulated DEGs.ConclusionThis study provides a reference transcriptome of P. ovis in absence of a reference genome. The analysis of DEGs and putative allergen genes may lay the foundation for studies of functional genomics, immunity and gene expression profiles of this parasitic mite species.Electronic supplementary materialThe online version of this article (doi:10.1186/s13071-016-1856-z) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

et al. 2016

Parasites Vectors

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“…Schistosomula have been used in transcriptome studies since the era of DNA microarrays [10, 21, 22], and until more recent times when NGS techniques [11, 23, 24] are used. These studies compared gene expression among schistosomula submitted to different lengths of time of culture and at different lifecycle stages of the parasite, but none of them attempted to simulate the intravascular condition of a vertebrate host.…”

Section: Discussionmentioning

confidence: 99%

Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum

et al. 2017

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Once inside a vertebrate host after infection, individual schistosomula of the parasite Schistosoma mansoni find a new and complex environment, which requires quick adjustments for survival, such as those that allow it to avoid the innate immune response of the host. Thus, it is very important for the parasite to remain within the skin after entering the host for a period of about 3 days, at which time it can then reach the venous system, migrate to the lungs and, by the end of eighth day post-infection, it reach the portal venous system, while undergoing minimal changes in morphology. However, after just a few days in the portal blood system, the parasite experiences an extraordinary increase in biomass and significant morphological alterations. Therefore, determining the constituents of the portal venous system that may trigger these changes that causes the parasite to consolidate its development inside the vertebrate host, thus causing the disease schistosomiasis, is essential. The present work simulated the conditions found in the portal venous system of the vertebrate host by exposing schistosomula of S. mansoni to in vitro culture in the presence of portal serum of the hamster, Mesocricetus auratus. Two different incubation periods were evaluated, one of 3 hours and one of 12 hours. These time periods were used to mimic the early contact of the parasite with portal serum during the course of natural infection. As a control, parasites were incubated in presence of hamster peripheral serum, in order to compare gene expression signatures between the two conditions. The mRNA obtained from parasites cultured under both conditions were submitted to a whole transcriptome library preparation and sequenced with a next generation platform. On average, nearly 15 million reads were produced per sample and, for the purpose of gene expression quantification, only reads mapped to one location of the transcriptome were considered. After statistical analysis, we found 103 genes differentially expressed by schistosomula cultured for 3 hours and 12 hours in the presence of hamster portal serum. After the subtraction of a second list of genes, also differentially expressed between schistosomula cultured for 3 hours and 12 hours in presence of peripheral serum, a set of 58 genes was finally established. This pattern was further validated for a subset of 17 genes, by measuring gene expression through quantitative real time polymerase chain reaction (qPCR). Processes that were activated by the portal serum stimulus include response to stress, membrane transport, protein synthesis and folding/degradation, signaling, cytoskeleton arrangement, cell adhesion and nucleotide synthesis. Additionally, a smaller number of genes down-regulated under the same condition act on cholinergic signaling, inorganic cation and organic anion membrane transport, cell adhesion and cytoskeleton arrangement. Considering the role of these genes in triggering processes that allow the parasite to quickly adapt, escape the immune response of the host and st...

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“…These genes can be divided into 18 families, which can be further divided into two groups, based on the number of members they contain [67]. Further transcriptome sequencing may have identified a further 11 micro-exon containing genes [69]. Protein expression studies show these genes code for small proteins with isoforms that have very similar size and charge that appear to be secreted.…”

Section: Alternate Splicing In Protozoan Parasitesmentioning

confidence: 99%

The role played by alternative splicing in antigenic variability in human endo-parasites

Hull

Dlamini

2014

Parasites & Vectors

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Endo-parasites that affect humans include Plasmodium, the causative agent of malaria, which remains one of the leading causes of death in human beings. Despite decades of research, vaccines to this and other endo-parasites remain elusive. This is in part due to the hyper-variability of the parasites surface proteins. Generally these surface proteins are encoded by a large family of genes, with only one being dominantly expressed at certain life stages. Another layer of complexity can be introduced through the alternative splicing of these surface proteins. The resulting isoforms may differ from each other with regard to cell localisation, substrate affinities and functions. They may even differ in structure to the extent that they are no longer recognised by the host’s immune system. In many cases this leads to changes in the N terminus of these proteins. The geographical localisation of endo-parasitic infections around the tropics and the highest incidences of HIV-1 infection in the same areas, adds a further layer of complexity as parasitic infections affect the host immune system resulting in higher HIV infection rates, faster disease progression, and an increase in the severity of infections and complications in HIV diagnosis. This review discusses some examples of parasite surface proteins that are alternatively spliced in trypanosomes, Plasmodium and the parasitic worm Schistosoma as well as what role alternate splicing may play in the interaction between HIV and these endo-parasites.

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Exploring the Schistosoma mansoni adult male transcriptome using RNA-seq

Cited by 37 publications

References 30 publications

Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

Preliminary analysis of Psoroptes ovis transcriptome in different developmental stages

Comparative sequence analysis reveals regulation of genes in developing schistosomula of Schistosoma mansoni exposed to host portal serum

The role played by alternative splicing in antigenic variability in human endo-parasites

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