Exploring the role of the ovary‐serine protease gene in the female fertility of the diamondback moth using CRISPR/Cas9
Muhammad Asad,
Jianying Liao,
Jing Chen
et al.
Abstract:BACKGROUNDOogenesis is a complex pathway necessary for proper female reproduction in insects. Ovary‐serine protease (Osp) is a homologous gene of serine protease Nudel (SpNudel) and plays an essential role in the oogenesis and ovary development of Drosophila melanogaster. However, the function of Osp is not determined in Plutella xylostella, a highly destructive pest of cruciferous crops.RESULTSThe PxOsp gene comprised a 5883‐bp open‐reading frame that encoded a protein consisting of 1994 amino acids, which co… Show more
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