Exploring the relationship between serum biomarkers, acute intracerebral changes and outcome after severe traumatic brain injury (TBI)

Childs, Charmaine; Martínez‐Morillo, Eduardo; Wai, Aung Phyo; Zu, Mya Myint; Diamandis, Anastasia; Diamandis, Eleftherios P.

doi:10.1515/cclm-2013-0216

Cited by 3 publications

(2 citation statements)

References 4 publications

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“…These were serum samples from patients (4 female and 8 male), aged 21 -78 years, recruited for a previous study [15] . Outcome at 3 months a er injury was assessed using the Glasgow Outcome Score (GOS).…”

Section: Serum Samples From Patients With Tbimentioning

confidence: 99%

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Martínez‐Morillo¹,

Childs

García

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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Brain injury is a medical emergency that needs to be diagnosed and treated promptly. Several proteins have been studied as biomarkers of this medical condition. The aims of this study were to: 1) evaluate the selectivity and precision of a commercial ELISA kit for neurofilament medium polypeptide (NFM) protein; and 2) evaluate the concentration in cerebrospinal fluid (CSF) and serum of healthy individuals and patients with brain damage.An ELISA from Elabscience was used. The selectivity was evaluated using size-exclusion chromatography and mass spectrometry. Intra- and inter-batch coefficients of variation (CV) were also studied. Fifty-one CSF samples from 36 age-matched patients with hemorrhagic stroke (HS) (n=30), ischemic stroke (IS) (n=11) and healthy individuals (n=10) were assayed. In addition, serum samples from healthy volunteers (n=47), 68 serum samples from seven patients with HS, 106 serum samples from 12 patients with traumatic brain injury (TBI) and 68 serum samples from 68 patients with mild traumatic brain injury (mTBI) were also analyzed.NFM was identified in the chromatographic fraction with highest immunoreactivity. The intra- and inter-batch CVs were ≤10% and ≤13%, respectively. The CSF-NFM concentration in HS was significantly higher (p<0.0001) than in IS and controls. Serum NFM concentration ranged from 0.26 to 8.57 ng/mL in healthy individuals (median=2.29), from 0.97 to 42.4 ng/mL in HS (median=10.8) and from 3.48 to 45.4 ng/mL in TBI (median=14.7). Finally, 44% of patients with mTBI had increased NFM concentration, with significantly higher levels (p=0.01) in patients with polytrauma.To our knowledge this is the first study describing increased NFM levels in CSF and serum from patients with brain damage.

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Section: Serum Samples From Patients With Tbimentioning

confidence: 99%

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Martínez‐Morillo¹,

Childs

García

et al. 2015

Clinical Chemistry and Laboratory Medicine (CCLM)

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“…Importantly, it was recently demonstrated that the high negative predictive values of protein S100B at ED admission would safely allow withholding CT scans in up to 50% of patients with mild TBI, so decreasing the total healthcare cost for patient management by approximately 30% [11]. It is also noteworthy that the measurement of S100B has consistently overwhelmed that of other potential biomarkers of brain injury, especially neuron-specific enolase (NSE) [12,13].…”

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confidence: 99%

Protein S100B: from cancer diagnostics to the evaluation of mild traumatic brain injury

Lippi

Cervellin

2016

Clinical Chemistry and Laboratory Medicine (CCLM)

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Traumatic Optic Nerve Injury Elevates Plasma Biomarkers of Traumatic Brain Injury in a Porcine Model

Bramblett

Harris

Scott

et al. 2021

Journal of Neurotrauma

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A diagnosis of traumatic brain injury (TBI) is typically based on patient medical history, a clinical examination, and imaging tests. Elevated plasma levels of glial fibrillary acidic protein (GFAP), ubiquitin c-terminal hydrolase L1 (UCH-L1), and neurofilament light chain (NFL) have been observed in numerous studies of TBI patients. It is reasonable to view traumatic optic neuropathy (TON) as a focal form of TBI. The purpose of this study was to assess if circulating GFAP, UCH-L1, and NFL are also elevated in a porcine model of TON. Serum levels of GFAP, UCH-L1, and NFL were measured immediately before optic nerve crush and 1 h post-injury in 10 Yucatan minipigs. Severity of optic nerve crush was confirmed by visual inspection of the optic nerve at time of injury, loss of visual function as measured by flash visual evoked potential (fVEP) at 7 and 14 days, and histological analysis of axonal transport of cholera toxin-β (CT-β) within the optic nerve. Post-crush concentrations of GFAP, UCH-L1, and NFL were all significantly elevated compared with pre-crush concentrations ( p < 0.01, p = 0.01, and p < 0.01, respectively). The largest increase was observed for GFAP with the post-injury median concentration increasing nearly sevenfold. The use of these TBI biomarkers for diagnosing and managing TON may be helpful for non-ophthalmologists in particular in diagnosing this condition. In addition, the potential utility of these biomarkers for diagnosing other optic nerve and/or retinal pathologies should be evaluated.

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Exploring the relationship between serum biomarkers, acute intracerebral changes and outcome after severe traumatic brain injury (TBI)

Cited by 3 publications

References 4 publications

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Neurofilament medium polypeptide (NFM) protein concentration is increased in CSF and serum samples from patients with brain injury

Protein S100B: from cancer diagnostics to the evaluation of mild traumatic brain injury

Traumatic Optic Nerve Injury Elevates Plasma Biomarkers of Traumatic Brain Injury in a Porcine Model

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