Exploring the relationship between polymorphisms of leptin and IGF-1 genes with milk yield in indicine and taurine crossbred cows

Dar, Mohammad Rayees; Singh, Mahendra; Thakur, S.S.

doi:10.1007/s11250-021-02866-1

Cited by 4 publications

(1 citation statement)

References 31 publications

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“…Rambachan et al (2019) found an association of LEP gene with these traits in Hariana cattle. Dar et al (2021) also found an association of the LEP gene with milk production traits in different cattle breeds. The genotypic patterns of exon-7 of STAT5A gene had a significant (P≤0.05) effect on PY and a non-significant (P>0.05) effect on TMY, and LL.…”

Section: Resultsmentioning

confidence: 78%

Study of milk production genes and their association with production traits in Rathi cattle

CHOUHAN

Pannu

JOSHI

et al. 2023

Indian J of Anim Sci

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The study aimed to identify polymorphism of LEP, and STAT5A milk-producing genes and their association with production traits in Rathi cattle. An overall 160 animals were selected from a population of Rathi cattle from Livestock Research Station, Rajasthan University of Veterinary and Animals Sciences, Bikaner (Rajasthan). The phenotypic information on total milk yield, peak yield and lactation length were recorded from the years 2012- 2018. Each animal’s milk sample (100 ml) was analysed for milk composition parameters. Genomic DNA was extracted from the whole blood sample through the spin column method and association analysis was done. The polymorphism have been observed in exon-3 (454 bp) of LEP gene, intron 9-10 (224 bp) and intron-15-exon-16 (379 bp) of STAT5A genes by PCR-SSCP and revealed two types of genotypic pattern. The STAT5A exon-7 (215 bp) also showed polymorphism with three genotypic patterns. The AB pattern of LEP exon-3 and AA pattern of intron 9-10 and intron-15-exon-16 of STAT5A genes showed a significant effect on total milk yield. The BB genotypic pattern of the STAT5A exon-7 gene showed a significant impact on peak yield. The AB pattern of LEP and STAT5A genes were associated with more milk fat % in the studied population of Rathi cattle. All these genes showed a significant effect on total solids. The association analysis of LEP and STAT5A genes with different milk production and milk composition traits illustrated the worth of these genes for marker-assisted selection of dairy cattle. Thus, the present study was designed to unmask the relevant genetic factors responsible for variation in milk performance traits.

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Section: Resultsmentioning

confidence: 78%

Study of milk production genes and their association with production traits in Rathi cattle

CHOUHAN

Pannu

JOSHI

et al. 2023

Indian J of Anim Sci

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Genetic Polymorphism of GH and IGF-1 Genes and Body Measurements Traits in Maghrabi Camel

Darwish,

Abdelhafez,

Othman

et al. 2024

Biol Bull Russ Acad Sci

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IGF-I and GH Genes polymorphism and their association with milk yields, composition and reproductive performance in Holstein–Friesian dairy cattle

Saleh,

Hassan,

EL-Hedainy

et al. 2024

BMC Vet Res

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Background The insulin-like growth factor (IGF-I) and growth hormone (GH) genes have been identified as major regulators of milk yield and composition, and reproductive performance in cattle. Genetic variations/polymorphism in these genes have been found to influence milk production, yield and quality. This investigation aimed to explore the association between IGF-I and GH polymorphisms and milk yield and composition, and reproductive performance in a herd consisting of 1000 Holstein–Friesian (HF) dairy cattle from El-Alamia farm. The experimental animals were 76 ± 7.25 months in age, with an average live weight of 750 ± 50.49 kg, and raised under the same conditions of feeding and weather. The studied animals were divided into three categories; high producers (n = 280), medium producers (n = 318) and low producers (n = 402). Results The digestion of 249 bp for IGF-I-SnaBI using the Restriction-fragment-length-polymorphism (RFLP) technique yielded two alleles; T (0.59) and C (0.41) and three genotypes; TT (0.52), TC (0.39) and CC (0.09) and this agrees with the results of DNA/gene sequencing technique. The sequencing analysis of the IGF-I gene revealed polymorphism in position 472 (C > T). Nucleotide sequencing of the amplified fragment of the IGF-I gene of different genotypes was done and submitted to the NCBI GenBank with Accession no. MH156812.1 and MH156811.1. While the digestion of 432 bp for GH-AluI using the RFLP technique yielded two alleles; A (0.81) and G (0.19) and two genotypes; AA (0.77) and AG (0.23) and this agrees with the results of DNA/gene sequencing technique. The sequencing analysis of the GH gene revealed polymorphism in the position 1758 C > G and in turn led to changes in amino acid sequence as Alanine for (A) compared to Glycine for (G). Nucleotide sequencing of the amplified fragment of the GH gene was done and submitted to the NCBI GenBank with Accession no. MH156810.1. The results of this study demonstrate the effects of variants of the GH-IGF-I somatotrophic axis on milk production and composition traits in commercial HF cattle. The greatest values of milk yield and reproductive performance were observed on IGF-I-SnaBI-TC and GH-AluI-AG genotypes. While the greatest % fat and % protein values were observed on IGF-I-SnaBI-CC and GH-AluI-AA genotyped individuals. Conclusion The genetic variation of the studied genes can be utilized in selecting animals with superior milk yield, composition and reproductive performance in Holstein–Friesian Dairy Cattle under subtropical conditions.

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Exploring the relationship between polymorphisms of leptin and IGF-1 genes with milk yield in indicine and taurine crossbred cows

Cited by 4 publications

References 31 publications

Study of milk production genes and their association with production traits in Rathi cattle

Study of milk production genes and their association with production traits in Rathi cattle

Genetic Polymorphism of GH and IGF-1 Genes and Body Measurements Traits in Maghrabi Camel

IGF-I and GH Genes polymorphism and their association with milk yields, composition and reproductive performance in Holstein–Friesian dairy cattle

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