Exploring the protist microbiome: The diversity of bacterial communities associated with Arcella spp. (Tubulina: Amoebozoa)

Gomaa, Fatma; Utter, Daniel R.; Loo, Wesley T.; Lahr, Daniel J. G.; Cavanaugh, Colleen M.

doi:10.1016/j.ejop.2021.125861

Cited by 5 publications

(2 citation statements)

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“…An open question remains regarding the persistence of hypolimnion-associated lineages during the biannual water column mixing in spring and autumn. Most likely their refuge is in the sediments, often inhabited by obligately anoxic benthic microbiomes (Bernhard et al, 2014; Gomaa et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Seasonal dynamics and drivers of microbial communities in a temperate dimictic lake: insights from metabarcoding and machine learning

Michał,

Anna,

Paweł

et al. 2024

Preprint

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Microbial communities, consisting of prokaryotes and protists, play a central role in ecological processes in aquatic environments. To understand these communities, metabarcoding provides a powerful tool to assess their taxonomic composition and to track spatio-temporal dynamics in both marine and freshwater environments. While previous research has primarily focused on marine ecosystems, it is important to study microbial communities in freshwater environments, which are characterised by high diversity and susceptibility to rapid environmental change. In temperate lakes, despite extensive research on temporal changes in physico-chemical factors and microscopic studies of plankton, there is a notable research gap regarding their eukaryotic microbial communities. Our study fills this gap by investigating the diversity and seasonal changes of prokaryotic and eukaryotic communities in Lake Ros (Poland), a representative temperate lake characterised by two mixing episodes in spring and autumn and pronounced stratification in summer. Our metabarcoding analysis revealed that both the bacterial and protist communities exhibit distinct seasonal patterns that are not necessarily shaped by dominant taxa. To decipher the drivers of the seasonal communities, we used machine learning and statistical methods and identified crucial amplicon sequence variants (ASVs) specific to each season. In addition, we identified a distinct community in the anoxic hypolimnion. We have also shown that the key factors shaping the community composition in Lake Ros are temperature, oxygen and silicon concentration. Understanding these community structures and the underlying factors is crucial in the context of climate change, which might affect mixing patterns and lead to prolonged stratification. Given the pronounced seasonal shifts observed in these communities, we can anticipate that climate change will profoundly impact the functioning of temperate dimictic lakes.

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Section: Discussionmentioning

confidence: 99%

Seasonal dynamics and drivers of microbial communities in a temperate dimictic lake: insights from metabarcoding and machine learning

Michał,

Anna,

Paweł

et al. 2024

Preprint

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“…Over the past decade, there has been a significant expansion in research exploring the relationship between hosts and their microbiota. As a result, investigations into microbiota have extended beyond humans to encompass other vertebrates, plants, invertebrates, and protists (Ahern et al, 2021;Akyol et al, 2020;Gomaa et al, 2022;van Oppen & Blackall, 2019;Vernier et al, 2020). Across the animal kingdom, substantial research on host-associated microbiota started providing compelling evidence for the widespread prevalence of phylosymbiosis.…”

Section: Introductionmentioning

confidence: 99%

Microbiota associated with meiofaunal nemerteans reveals evidence of phylosymbiosis

Leasi,

Eckert,

Norenburg

et al. 2023

Preprint

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Phylosymbiosis, the association between the phylogenetic relatedness of hosts and the composition of their microbial communities, is a widespread phenomenon in diverse animal taxa. However, the generality of the existence of such a pattern has been questioned, and there seems evidence against its occurrence in small-sized aquatic animals, for which the microbiota composition is mostly shaped by local environmental factors. This study aims to investigate the microbial communities associated with poorly known marine interstitial nemerteans to uncover their microbiota diversity and assess the occurrence of phylosymbiosis. Through a comprehensive approach, specimens from various Central American sites were analyzed using morphology-based taxonomy and molecular techniques targeting the host 18S rRNA gene whereas their microbial association was analyzed by targeting the prokaryotic 16S rRNA gene. Phylogenetic and statistical analyses were conducted to examine the potential effects of host nemertean taxa and sampling locations on the host-associated microbial communities. The results provide compelling evidence of phylosymbiosis in meiofaunal nemerteans, emphasizing the significant impact of host genetic relatedness on microbiome diversity also in small-sized animals. Additional research is needed to fully unravel potential symbiotic relationships as well as the complex mechanisms that govern the relationships between hosts and their microbiota across various organisms and ecological settings. The remarkable diversity of meiofaunal animals, spanning various animal phyla with different lifestyles and inhabiting diverse ecosystems, combined with the advancements in multi-omics approaches, offers a promising avenue for comprehensive understanding of the evolutionary and ecological interactions between hosts and their microbiota throughout the animal tree of life.

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Protists’ microbiome: A fine-scale, snap-shot field study on the ciliate Euplotes

Manassero

Vannini

2023

European Journal of Protistology

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Exploring the protist microbiome: The diversity of bacterial communities associated with Arcella spp. (Tubulina: Amoebozoa)

Cited by 5 publications

References 50 publications

Seasonal dynamics and drivers of microbial communities in a temperate dimictic lake: insights from metabarcoding and machine learning

Seasonal dynamics and drivers of microbial communities in a temperate dimictic lake: insights from metabarcoding and machine learning

Microbiota associated with meiofaunal nemerteans reveals evidence of phylosymbiosis

Protists’ microbiome: A fine-scale, snap-shot field study on the ciliate Euplotes

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