Exploring the potential of slaughterhouse waste valorization: Development and scale-up of a new bioprocess for medium-chain length polyhydroxyalkanoates production

Acedos, Miguel G.; Moreno-Cid, Juan A.; Verdú, Fuensanta; González, José Antonio García-Cruces; Tena, Sara; López, Juan Carlos

doi:10.1016/j.chemosphere.2021.132401

Cited by 11 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, it is essential to choose substrates like agro-industrial wastes that meet basic requirements, such as high quality and availability throughout the year, suitability for storage, incompetency with other feedstock applications, and accessible collection and transportation. Optimally, PHA production should be integrated into existing industrial production lines with cheap, renewable, and high-quality substrates. ,− …”

Section: Biosynthesis Of Phamentioning

confidence: 99%

Polyhydroxyalkanoates for Sustainable Aquaculture: A Review of Recent Advancements, Challenges, and Future Directions

Asiri

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

Polyhydroxyalkanoates (PHA) are biodegradable biopolymers produced by prokaryotic microbes, which, at the same time, can be applied as single-cell proteins (SCPs), growing on renewable waste-derived substrates. These PHA polymers have gained increasing attention as a sustainable alternative to conventional plastics. One promising application of PHA and PHA-rich SCPs lies within the aquaculture food industry, where they hold potential as feed additives, biocontrol agents against diseases, and immunostimulants. Nevertheless, the cost of PHA production and application remains high, partly due to expensive substrates for cultivating PHA-accumulating SCPs, costly sterilization, energy-intensive SCPs harvesting techniques, and toxic PHA extraction and purification processes. This review summarizes the current state of PHA production and its application in aquaculture. The structure and classification of PHA, microbial sources, cultivation substrates, biosynthesis pathways, and the production challenges and solutions are discussed. Next, the potential of PHA application in aquaculture is explored, focusing on aquaculture challenges, common and innovative PHA-integrated farming practices, and PHA mechanisms in inhibiting pathogens, enhancing the immune system, and improving growth and gut health of various aquatic species. Finally, challenges and future research needs for PHA production and application in aquaculture are identified. Overall, this review paper provides a comprehensive overview of the potential of PHA in aquaculture and highlights the need for further research in this area.

show abstract

Section: Biosynthesis Of Phamentioning

confidence: 99%

Polyhydroxyalkanoates for Sustainable Aquaculture: A Review of Recent Advancements, Challenges, and Future Directions

Asiri

2024

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

“…Conversion of waste animal fats and mixed fat fractions to PHA has been demonstrated in multiple studies at the shake flask scale (Acedos et al, 2022 ; Ashby & Foglia, 1998 ; Cromwick et al, 1996 ; Riedel et al, 2015 ; Rodríguez et al, 2021 ; Saad et al, 2021 ; Taniguchi et al, 2003 ). A subsequent process transfer to the bioreactor scale is challenging due to the hydrophobicity and high melting temperature of waste animal fats.…”

Section: Introductionmentioning

confidence: 99%

Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions

Gutschmann

Högl

Huang

et al. 2022

Microbial Biotechnology

View full text Add to dashboard Cite

Fat‐containing animal by‐product streams are locally available in large quantities. Depending on their quality, they can be inexpensive substrates for biotechnological processes. To accelerate industrial polyhydroxyalkanoate (PHA) bioplastic production, the development of efficient bioprocesses that are based on animal by‐product streams is a promising approach to reduce overall production costs. However, the solid nature of animal by‐product streams requires a tailor‐made process development. In this study, a fat/protein‐emulsion (FPE), which is a by‐product stream from industrial‐scale pharmaceutical heparin production and of which several hundred tons are available annually, was evaluated for PHA production with Ralstonia eutropha . The FPE was used as the sole source of carbon and nitrogen in shake flask and bioreactor cultivations. A tailored pneumatic feeding system was built for laboratory bioreactors to facilitate fed‐batch cultivations with the solid FPE. The process yielded up to 51 g L −1 cell dry weight containing 71 wt% PHA with a space–time yield of 0.6 g PHA L −1 h −1 without using any carbon or nitrogen sources other than FPE. The presented approach highlights the potential of animal by‐product stream valorization into PHA and contributes to a transition towards a circular bioeconomy.

show abstract

“…The production of PHAs by bacteria is dependent on several factors such as species of bacteria, type of carbon source, cultivation period, pH, temperature, the ratio of carbon to nitrogen sources, and the availability of macro- and microelements (e.g., phosphorus, sulfur, and magnesium). − Moreover, cultivations can be executed by different fermentation strategies, including batch, fed-batch, and continuous processes to accomplish a high growth rate, a high content of biopolymer accumulation in bacterial cells, and optimize PHA yield within a short time …”

Section: Introductionmentioning

confidence: 99%

A Review of Current Achievements and Recent Challenges in Bacterial Medium-Chain-Length Polyhydroxyalkanoates: Production and Potential Applications

Azizi,

Eslami,

Goudarzi

et al. 2024

Biomacromolecules

View full text Add to dashboard Cite

Using petroleum-derived plastics has contributed significantly to environmental issues, such as greenhouse gas emissions and the accumulation of plastic waste in ecosystems.Researchers have focused on developing ecofriendly polymers as alternatives to traditional plastics to address these concerns. This review provides a comprehensive overview of medium-chain-length polyhydroxyalkanoates (mcl-PHAs), biodegradable biopolymers produced by microorganisms that show promise in replacing conventional plastics. The review discusses the classification, properties, and potential substrates of less studied mcl-PHAs, highlighting their greater ductility and flexibility compared to poly(3hydroxybutyrate), a well-known but brittle PHA. The authors summarize existing research to emphasize the potential applications of mcl-PHAs in biomedicine, packaging, biocomposites, water treatment, and energy. Future research should focus on improving production techniques, ensuring economic viability, and addressing challenges associated with industrial implementation. Investigating the biodegradability, stability, mechanical properties, durability, and cost-effectiveness of mcl-PHA-based products compared to petroleum-based counterparts is crucial. The future of mcl-PHAs looks promising, with continued research expected to optimize production techniques, enhance material properties, and expand applications. Interdisciplinary collaborations among microbiologists, engineers, chemists, and materials scientists will drive progress in this field. In conclusion, this review serves as a valuable resource to understand mcl-PHAs as sustainable alternatives to conventional plastics. However, further research is needed to optimize production methods, evaluate long-term ecological impacts, and assess the feasibility and viability in various industries.

show abstract

Exploring the potential of slaughterhouse waste valorization: Development and scale-up of a new bioprocess for medium-chain length polyhydroxyalkanoates production

Cited by 11 publications

References 41 publications

Polyhydroxyalkanoates for Sustainable Aquaculture: A Review of Recent Advancements, Challenges, and Future Directions

Polyhydroxyalkanoates for Sustainable Aquaculture: A Review of Recent Advancements, Challenges, and Future Directions

Polyhydroxyalkanoate production from animal by‐products: Development of a pneumatic feeding system for solid fat/protein‐emulsions

A Review of Current Achievements and Recent Challenges in Bacterial Medium-Chain-Length Polyhydroxyalkanoates: Production and Potential Applications

Contact Info

Product

Resources

About