Exploring the potential of microalgae in the recycling of dairy wastes

Gramegna, Giovanna; Scortica, Anna; Scafati, Valentina; Ferella, Francesco; Gurrieri, Libero; Giovannoni, Moira; Bassi, Roberto; Sparla, Francesca; Mattei, Benedetta; Benedetti, Manuel

doi:10.1016/j.biteb.2020.100604

Cited by 38 publications

(14 citation statements)

References 43 publications

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“…This was due microalgae did not have the ability to digest excessive nutrients in VCO waste, thereby leading to the possibility of producing toxic metabolites [26,27]. Likewise, microbial load in the effluent also had a role to render growth rate as they competed to consume the nutrients [20]. Based on Table S3, the highest growth rate was obtained at 20% effluent concentration in accordance with Fig.…”

Section: Effect Of Vco Mill Effluent On Biomass and Growth Rate Of Bo...supporting

confidence: 64%

“…Many studies also have reported the increasing in microalgae biomass by mixotrophic and utilization of glycerol during cultivation as followed: Chlorella vulgaris in real centrate wastewater [9], Chlorella in dairy wastes [20], C. protothecoides in dairy wastes [11,12], Chlorella sp. in palm oil mill effluent [21], Phaeodactylum tricornitum and Chlorella vulgaris with glycerol addition as carbon source [19,22].…”

Section: Introductionmentioning

confidence: 99%

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Enhancing the biomass production of microalgae by mixotrophic cultivation using virgin coconut oil mill effluent

Dewi¹,

Mahreni²,

Nur³

et al. 2022

Environmental Engineering Research

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Skim is a major by-product of the virgin coconut oil (VCO) industry consisting of nutrients and is currently discharged to the environment as waste. Hence, this research aimed to utilize skim as a medium for Botryococcus braunii and Chlorella vulgaris growth. The effect of organic carbon resourced from glucose and glycerol on biomass was also investigated at certain concentrations with intervals 100:0 to 0:100 (%). Results indicated that Botryococcus braunii and Chlorella vulgaris cultivated under 20% VCO mill effluent gave the highest biomass production, which accounts for 1.69 g/L and 5.34 g/L; 89.40% and 86.70% COD reduction efficiency. Whereas, mixotrophic condition showed a positive trend for Botryococcus braunii at 80:20 (glucose:glycerol) with 5.60 g/L biomass production and 97.64% COD removal efficiency. Vice versa, the addition of glucose:glycerol gave an inhibitory impact on Chlorella vulgaris as the biomass production was merely 1.66 g/L with COD efficiency 94.03%.

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Section: Effect Of Vco Mill Effluent On Biomass and Growth Rate Of Bo...supporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Enhancing the biomass production of microalgae by mixotrophic cultivation using virgin coconut oil mill effluent

Dewi¹,

Mahreni²,

Nur³

et al. 2022

Environmental Engineering Research

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“…Chlorella vulgaris wild-type strain 211-11p was obtained from the Culture Collection of Algae (Göttingen University, Germany). C. vulgaris was maintained at 26 °C, with a 16/8 h light/dark photoperiod, light intensity of 35 µmol photons m −2 s −1 , in solid or liquid TAP medium on a rotary shaker (180 rpm) according to [ 22 ]. Growth was followed by measuring cell density using an automated cell counter (Countess II FL Cell Counter, ThermoFisher).…”

Section: Methodsmentioning

confidence: 99%

“…It is worth noting that Phi is only metabolizable by few chemolithotrophic bacteria possessing the phosphite dehydrogenase enzyme, such as Pseudomonas stutzeri [20], and therefore an eventual microbial growth in such medium could only be sustained at the expense of C. vulgaris biomass, used here as carbon and phosphate source. Moreover, Phi is characterized by a mild antimicrobial activity that made the selection in the algal trap more stringent [21][22][23]. In the absence of antimicrobial agents, several microorganisms could proliferate in the trap eating each other and exploiting the algal-supplemented medium as a mere basal salt source, hindering the discrimination of a real algivorous and algal saprophyte from the rest of contaminating microbes.…”

Section: Capture Of the Unknown Fungal Isolate By The Algal Trapmentioning

confidence: 99%

A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes

Giovannoni

Larini

Scafati

et al. 2021

Biotechnol Biofuels

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Background Microalgae are coming to the spotlight due to their potential applications in a wide number of fields ranging from the biofuel to the pharmaceutical sector. However, several factors such as low productivity, expensive harvesting procedures and difficult metabolite extractability limit their full utilization at industrial scale. Similarly to the successful employment of enzymatic arsenals from lignocellulolytic fungi to convert lignocellulose into fermentable sugars for bioethanol production, specific algalytic formulations could be used to improve the extractability of lipids from microalgae to produce biodiesel. Currently, the research areas related to algivorous organisms, algal saprophytes and the enzymes responsible for the hydrolysis of algal cell wall are still little explored. Results Here, an algal trap method for capturing actively growing microorganisms was successfully used to isolate a filamentous fungus, that was identified by whole-genome sequencing, assembly and annotation as a novel Penicilliumsumatraense isolate. The fungus, classified as P.sumatraense AQ67100, was able to assimilate heat-killed Chlorellavulgaris cells by an enzymatic arsenal composed of proteases such as dipeptidyl- and amino-peptidases, β-1,3-glucanases and glycosidases including α- and β-glucosidases, β-glucuronidase, α-mannosidases and β-galactosidases. The treatment of C.vulgaris with the filtrate from P.sumatraense AQ67100 increased the release of chlorophylls and lipids from the algal cells by 42.6 and 48.9%, respectively. Conclusions The improved lipid extractability from C.vulgaris biomass treated with the fungal filtrate highlighted the potential of algal saprophytes in the bioprocessing of microalgae, posing the basis for the sustainable transformation of algal metabolites into biofuel-related compounds.

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“…The environmental impact of the dairy industry is also manifested in the generation of large amounts of wastewater. It is estimated that the processing of one liter of milk produces 0.2-10 liters of wastewater (Gramegna et al, 2020). Dairy wastewater, as compared to municipal or domestic wastewater, is characterized by a higher content of organic substances, mainly nitrogen and phosphorus compounds, which -if discharged into the environment without appropriate treatment -increases the risk of eutrophication of water reservoirs and watercourses (Kolev Slavov, 2017).…”

Section: (3) 2022mentioning

confidence: 99%

Determinants of Implementation of the Circular Economy in the Food Processing Sector on the Example of the Dairy Industry

Gralak¹,

Grochowska²,

Szczepaniak³

2022

Zagadnienia Ekonomiki Rolnej / Problems of Agricultural Economi

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Exploring the potential of microalgae in the recycling of dairy wastes

Cited by 38 publications

References 43 publications

Enhancing the biomass production of microalgae by mixotrophic cultivation using virgin coconut oil mill effluent

Enhancing the biomass production of microalgae by mixotrophic cultivation using virgin coconut oil mill effluent

A novel Penicillium sumatraense isolate reveals an arsenal of degrading enzymes exploitable in algal bio-refinery processes

Determinants of Implementation of the Circular Economy in the Food Processing Sector on the Example of the Dairy Industry

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