Exploring the Potential of Electrical Impedance Tomography for Tissue Engineering Applications

Wu, Haowei; Zhou, Wenli; Yang, Yunjie; Jia, Jiabin; Bagnaninchi, Pierre O.

doi:10.3390/ma11060930

Cited by 33 publications

(32 citation statements)

References 40 publications

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“…The successful use of EIT as a medical diagnostic and its ability to obtain in vivo information, suggests that it may be an effective monitoring technique for future 3D cell cultures. Researchers have demonstrated the measurement of 3D tissue impedance and subsequently reconstruction of the respective images using EIT (Jamil et al, 2016; Wu, Yang, Bagnaninchi, & Jia, 2017; Wu, Yang, Bagnaninchi, & Jia, 2018; Wu, Zhou, Yang, Jia, & Bagnaninchi, 2018; Yang et al, 2017; Yin, Wu, Jia, & Yang, 2018; Yin, Yang, Jia, & Tan, 2017). For monitoring 3D cell aggregates or spheroids, two electrode distribution designs were implemented.…”

Section: D Impedance Tomographymentioning

confidence: 99%

“…(b) Rectangular electrodes at the boundary of the well were used to monitor spheroid and (c) reconstructed images for the spheroid samples in 2% Triton X‐100 solution (1‐2) and HG culture medium (3–4). Reproduced with permission (Wu, Yang et al, 2018; Wu, Zhou et al, 2018). Copyright Wu et al (2018) [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: D Impedance Tomographymentioning

confidence: 99%

“…Reproduced with permission (Wu, Yang et al, 2018; Wu, Zhou et al, 2018). Copyright Wu et al (2018) [Color figure can be viewed at wileyonlinelibrary.com]…”

Section: D Impedance Tomographymentioning

confidence: 99%

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Three‐Dimensional (3D) cell culture monitoring: Opportunities and challenges for impedance spectroscopy

León

Pupovac

McArthur

2020

Biotech & Bioengineering

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Three‐dimensional (3D) cell culture has developed rapidly over the past 5–10 years with the goal of better replicating human physiology and tissue complexity in the laboratory. Quantifying cellular responses is fundamental in understanding how cells and tissues respond during their growth cycle and in response to external stimuli. There is a need to develop and validate tools that can give insight into cell number, viability, and distribution in real‐time, nondestructively and without the use of stains or other labelling processes. Impedance spectroscopy can address all of these challenges and is currently used both commercially and in academic laboratories to measure cellular processes in 2D cell culture systems. However, its use in 3D cultures is not straight forward due to the complexity of the electrical circuit model of 3D tissues. In addition, there are challenges in the design and integration of electrodes within 3D cell culture systems. Researchers have used a range of strategies to implement impedance spectroscopy in 3D systems. This review examines electrode design, integration, and outcomes of a range of impedance spectroscopy studies and multiparametric systems relevant to 3D cell cultures. While these systems provide whole culture data, impedance tomography approaches have shown how this technique can be used to achieve spatial resolution. This review demonstrates how impedance spectroscopy and tomography can be used to provide real‐time sensing in 3D cell cultures, but challenges remain in integrating electrodes without affecting cell culture functionality. If these challenges can be addressed and more realistic electrical models for 3D tissues developed, the implementation of impedance‐based systems will be able to provide real‐time, quantitative tracking of 3D cell culture systems.

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Section: D Impedance Tomographymentioning

confidence: 99%

Section: D Impedance Tomographymentioning

confidence: 99%

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Three‐Dimensional (3D) cell culture monitoring: Opportunities and challenges for impedance spectroscopy

León

Pupovac

McArthur

2020

Biotech & Bioengineering

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“…In the last decades, a huge interest has been addressed toward to possibility to translate both these techniques from 2D to 3D sensing [15,16]. This further methodological exploitation may ensure the monitoring of cells growth or differentiation with improved reliability, for both pharmacology and regenerative medicine purposes.…”

Section: Introductionmentioning

confidence: 99%

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

et al. 2019

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During the last years, scientific research in biotechnology has been reporting a considerable boost forward due to many advances marked in different technological areas. Researchers working in the field of regenerative medicine, mechanobiology and pharmacology have been constantly looking for non-invasive methods able to track tissue development, monitor biological processes and check effectiveness in treatments. The possibility to control cell cultures and quantify their products represents indeed one of the most promising and exciting hurdles. In this perspective, the use of conductive materials able to map cell activity in a three-dimensional environment represents the most interesting approach. The greatest potential of this strategy relies on the possibility to correlate measurable changes in electrical parameters with specific cell cycle events, without affecting their maturation process and considering a physiological-like setting. Up to now, several conductive materials has been identified and validated as possible solutions in scaffold development, but still few works have stressed the possibility to use conductive scaffolds for non-invasive electrical cell monitoring. In this picture, the main objective of this review was to define the state-of-the-art concerning conductive biomaterials to provide researchers with practical guidelines for developing specific applications addressing cell growth and differentiation monitoring. Therefore, a comprehensive review of all the available conductive biomaterials (polymers, carbon-based, and metals) was given in terms of their main electric characteristics and range of applications.

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“…Combining 3D in vitro human models with impedance based cellular assays will provide a platform for a non-invasive, quantitative, detailed analysis of cell culture in real time, hugely benefiting the in vitro drug screening process and making animal testing less attractive. Recent developments in microelectrode sensor design for electrical impedance tomography show great promise in their translation to the field of tissue engineering and 3D culture [70][71][72].…”

Section: Platforms and Microfluidic Systemsmentioning

confidence: 99%

Application of Impedance-Based Techniques in Hepatology Research

Morgan

Gamal

Samuel

et al. 2019

JCM

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There are a variety of end-point assays and techniques available to monitor hepatic cell cultures and study toxicity within in vitro models. These commonly focus on one aspect of cell metabolism and are often destructive to cells. Impedance-based cellular assays (IBCAs) assess biological functions of cell populations in real-time by measuring electrical impedance, which is the resistance to alternating current caused by the dielectric properties of proliferating of cells. While the uses of IBCA have been widely reported for a number of tissues, specific uses in the study of hepatic cell cultures have not been reported to date. IBCA monitors cellular behaviour throughout experimentation non-invasively without labelling or damage to cell cultures. The data extrapolated from IBCA can be correlated to biological events happening within the cell and therefore may inform drug toxicity studies or other applications within hepatic research. Because tight junctions comprise the blood/biliary barrier in hepatocytes, there are major consequences when these junctions are disrupted, as many pathologies centre around the bile canaliculi and flow of bile out of the liver. The application of IBCA in hepatology provides a unique opportunity to assess cellular polarity and patency of tight junctions, vital to maintaining normal hepatic function. Here, we describe how IBCAs have been applied to measuring the effect of viral infection, drug toxicity/IC50, cholangiopathies, cancer metastasis and monitoring of the gut-liver axis. We also highlight key areas of research where IBCAs could be used in future applications within the field of hepatology.

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Exploring the Potential of Electrical Impedance Tomography for Tissue Engineering Applications

Cited by 33 publications

References 40 publications

Three‐Dimensional (3D) cell culture monitoring: Opportunities and challenges for impedance spectroscopy

Three‐Dimensional (3D) cell culture monitoring: Opportunities and challenges for impedance spectroscopy

A Review on Biomaterials for 3D Conductive Scaffolds for Stimulating and Monitoring Cellular Activities

Application of Impedance-Based Techniques in Hepatology Research

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