Exploring the potential mechanism of Rhodomyrtus tomentosa (Ait.) Hassk fruit phenolic rich extract on ameliorating nonalcoholic fatty liver disease by integration of transcriptomics and metabolomics profiling

Wang, Ruimin; Yao, Linling; Lin, Xue; Hu, Xiaoping; Wang, Lu

doi:10.1016/j.foodres.2021.110824

Cited by 45 publications

(38 citation statements)

References 58 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The liver, pancreas and colon of the mice were collected for the preparation of paraffin tissue sections. The morphologies of the tissues were observed using a microscope (Nikon, Japan) after staining with hematoxylin and eosin (H&E) [ 28 ]. The histopathological score of the tissues was obtained according to the standard in Table S1 .…”

Section: Methodsmentioning

confidence: 99%

Kombucha Reduces Hyperglycemia in Type 2 Diabetes of Mice by Regulating Gut Microbiota and Its Metabolites

Wang

et al. 2022

Foods

View full text Add to dashboard Cite

Kombucha, which is rich in tea polyphenols and organic acid, is a kind of acidic tea soup beverage fermented by acetic acid bacteria, yeasts, lactic acid bacteria. Kombucha has been reported to possess anti-diabetic activity, but the underlying mechanism was not well understood. In this study, a high-fat, high-sugar diet combined with streptozotocin (STZ) injection was used to induce T2DM model in mice. After four weeks of kombucha intervention, the physiological and biochemical index were measured to determine the diabetes-related indicators. High-throughput sequencing technology was used to analyze the changes in gut microbiota from the feces. The results showed that four weeks of kombucha intervention increased the abundance of SCFAs-producing bacteria and reduced the abundance of gram-negative bacteria and pathogenic bacteria. The improvement in gut microbiota reduced the damage of intestinal barrier, thereby reducing the displacement of lipopolysaccharide (LPS) and inhibiting the occurrence of inflammation and insulin resistance in vivo. In addition, the increased levels of SCFAs-producing bacteria, and thus increasing the SCFAs, improved islet β cell function by promoting the secretion of gastrointestinal hormones (GLP-1/PYY). This study methodically uncovered the hypoglycemic mechanism of kombucha through gut microbiota intervention, and the result suggested that kombucha may be introduced as a new functional drink for T2DM prevention and treatment.

show abstract

Section: Methodsmentioning

confidence: 99%

Kombucha Reduces Hyperglycemia in Type 2 Diabetes of Mice by Regulating Gut Microbiota and Its Metabolites

Wang

et al. 2022

Foods

View full text Add to dashboard Cite

show abstract

“…Polyphenols, widely distributed in plants, are a very diverse and multifunctional group of phytochemicals, containing flavonoids, phenolic acids, anthocyanins, procyanidins, and stilbenes. Accumulating studies have shown that polyphenols have natural antioxidant properties and play an important role in preventing food oxidation and exerting health-promoting effects [1] , [2] . For these reasons, polyphenols or polyphenolic extracts from herbs, fruits, and vegetables have been wildly used in food, medicine, feed stuff and other fields [3] , [4] .…”

Section: Introductionmentioning

confidence: 99%

Ultrasonic-assisted extraction of polyphenolic compounds from Paederia scandens (Lour.) Merr. Using deep eutectic solvent: optimization, identification, and comparison with traditional methods

Liu

Wang

Zhang

et al. 2022

Ultrasonics Sonochemistry

View full text Add to dashboard Cite

“…GADD45G is an inflammation-related gene associated with oxidative stress and cytokine secretion signaling pathways. The expression of GADD45G is upregulated in the liver in high-fat-diet-induced non-alcoholic fatty liver disease (NAFLD), and its expression decreases when the high-fat condition is improved ( Wang et al, 2022 ), which may be related to its effect of on hepatic fibrosis and the occurrence of chemical transformation ( Hong et al, 2016 ). The above results suggest that differential expression of spliced isoforms may cause differences in gene expression.…”

Section: Discussionmentioning

confidence: 99%

Integrative Analysis of Nanopore and Illumina Sequencing Reveals Alternative Splicing Complexity in Pig Longissimus Dorsi Muscle

Shu

Wang

et al. 2022

Front. Genet.

View full text Add to dashboard Cite

Alternative splicing (AS) is a key step in the post-transcriptional regulation of gene expression that can affect intramuscular fat (IMF). In this study, longissimus dorsi muscles from 30 pigs in high- and low- IMF groups were used to perform Oxford Nanopore Technologies (ONT) full-length sequencing and Illumina strand-specific RNA-seq. A total of 43,688 full-length transcripts were identified, with 4,322 novel genes and 30,795 novel transcripts. Using AStalavista, a total of 14,728 AS events were detected in the longissimus dorsi muscle. About 17.79% of the genes produced splicing isoforms, in which exon skipping was the most frequent AS event. By analyzing the expression differences of mRNAs and splicing isoforms, we found that differentially expressed mRNAs with splicing isoforms could participate in skeletal muscle development and fatty acid metabolism, which might determine muscle-related traits. SERBP1, MYL1, TNNT3, and TNNT1 were identified with multiple splicing isoforms, with significant differences in expression. AS events occurring in IFI6 and GADD45G may cause significant differences in gene expression. Other AS events, such as ONT.15153.3, may regulate the function of ART1 by regulating the expression of different transcripts. Moreover, co-expression and protein-protein interaction (PPI) analysis indicated that several genes (MRPL27, AAR2, PYGM, PSMD4, SCNM1, and HNRNPDL) may be related to intramuscular fat. The splicing isoforms investigated in our research provide a reference for the study of alternative splicing regulation of intramuscular fat deposition.

show abstract

Exploring the potential mechanism of Rhodomyrtus tomentosa (Ait.) Hassk fruit phenolic rich extract on ameliorating nonalcoholic fatty liver disease by integration of transcriptomics and metabolomics profiling

Cited by 45 publications

References 58 publications

Kombucha Reduces Hyperglycemia in Type 2 Diabetes of Mice by Regulating Gut Microbiota and Its Metabolites

Kombucha Reduces Hyperglycemia in Type 2 Diabetes of Mice by Regulating Gut Microbiota and Its Metabolites

Ultrasonic-assisted extraction of polyphenolic compounds from Paederia scandens (Lour.) Merr. Using deep eutectic solvent: optimization, identification, and comparison with traditional methods

Integrative Analysis of Nanopore and Illumina Sequencing Reveals Alternative Splicing Complexity in Pig Longissimus Dorsi Muscle

Contact Info

Product

Resources

About