Exploring the potential immune cells related to the heredity of acute pancreatitis based on Mendelian randomization study

Abstract

Objective Through Mendelian randomization (MR) analysis method, exploring the potential innate immune cells associated with acute pancreatitis. Methods This study is based on publicly available genetic data, and selects SNP related to immune cells from the immune cell data set after filtering a series of steps, and matches SNP related to immune cells as covariates for MR analysis from the AP data set.Five regression model analysis methods, including MR Egger, weighted median (WME), inverse variance weighting (IVW), simple model, and weighted model, were used to analyze the causal relationship between these immune cells and AP, and to verify the diversity of results. ity, heterogeneity and robustness. Results This study found that 36 types of immune cell phenotypes have potential causal relationships with AP, and further correction revealed that 4 types of immune cells have causal relationships with AP, including CD14+ CD16- OR=0.93 (95%CI=0.899-0.970, P=0.00045), CD28 OR=0.87 (95%CI=0.801-0.937,P=0.00036),CD14+ OR=0.93 (95%CI=0.897-0.971,P=0.00068),Mo MDSC OR=1.07 (95%CI=1.030-1.113, P=0.00049).The study was assessed by IVW and MR-Egger tests (P>0.05), indicating that there was no heterogeneity in the study. After the MR-Egger intercept test P>0.05, it indicated that the data did not have multiple effects and the study results were robust. The leave-one-out method removed SNPs one by one and did not find SNPs that had a large impact on the causal association estimates, indicating that the results were robust. Conclusions Our study found by MR that increased levels of CD14+CD16-, CD28, CD14+ may be protective factors for AP, and increased level of Mo MDSC may be a risk factor for AP. These four types of immune cells are potential immune cells genetically associated with AP.