Exploring the pathogenesis of type 2 diabetes mellitus intestinal damp-heat syndrome and the therapeutic effect of Gegen Qinlian Decoction from the perspective of exosomal miRNA

He, Liyun; Bao, Tingting; Yang, Yingying; Wang, Han; Gu, Chengjuan; Chen, Jia; Zhai, Tiangang; He, Xiaozhou; Wu, Mengyi; Zhao, Linhua; Tong, Xiaolin

doi:10.1016/j.jep.2021.114786

Cited by 9 publications

(10 citation statements)

References 77 publications

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“…GGQL was first recorded in a famous ancient medicine treatise Shanghan Lun, compiled by Zhong-Jing Zhang in the Han Dynasty of Chinese history (202 BC-220 AD). In previous researches, it has been found that GGQL exerts a range of pharmacological activities, including anti-inflammation, antidiabetic, antioxidant, and immunoregulative effects [11][12][13][14]. Our research team has confirmed that GGQL could decrease the fasting blood glucose (FBG) in mice with diabetes and improve the oral glucose tolerance and insulin tolerance in rats with T2DM [15].…”

Section: Introductionmentioning

confidence: 59%

Exploring Anti-Type 2 Diabetes Mellitus Mechanism of Gegen Qinlian Decoction by Network Pharmacology and Experimental Validation

Bao

Sun

et al. 2022

Disease Markers

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Purpose. Gegen Qinlian Decoction (GGQL) has been employed to treat type 2 diabetes mellitus (T2DM) in the clinical practice of traditional Chinese medicine. However, the underlying mechanism of GGQL in the treatment of T2DM remains unknown. This study was aimed at exploring the pharmacological mechanisms of GGQL against T2DM via network pharmacology analysis combined with experimental validation. Methods. The effective components of GGQL were screened, and the target was predicted by using traditional Chinese medicine systems pharmacology database and analysis platform (TCMSP). The candidate targets of GGQL were predicted by network pharmacological analysis, and crucial targets were chosen by the protein-protein interaction (PPI) network. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) functional enrichment analyses were performed to predict the core targets and pathways of GGQL against T2DM. Then, T2DM mice were induced by a high-fat diet combined with streptozotocin. The model and GGQL groups were given normal saline and GGQL aqueous solution (10 and 20 g/kg/d) intragastric administration, respectively, for 8 weeks. The mice in the GGQLT groups were administered with GGQLT at 10 and 20 g/kg/d, respectively. The pathological changes in liver tissues were observed by hematoxylin-eosin staining. The protein expression of TNF-α and NF-κB was verified by western blotting. Results. A total of 204 common targets of GGQL for the treatment of T2DM were obtained from 140 active ingredients and 212 potential targets of T2DM. GO and KEGG enrichment analysis involved 119 signaling pathways, mainly in inflammatory TNF signaling pathways. Animal experiments showed that GGQL significantly reduced the serum levels of body mass, fasting blood glucose, fasting insulin, HOMA-IR, TNF-α, and IL-17. The liver pathological section showed that GGQL could improve the vacuolar degeneration and lipid deposition in the liver of T2DM mice. Mechanistically, GGQL downregulated the mRNA expression of TNF-α and NF-κB. Conclusions. This study demonstrated that GGQL may exert antidiabetic effects against T2DM by suppressing TNF-α signaling pathway activation, thus providing a basis for its potential use in clinical practice and further study in treating T2DM.

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Section: Introductionmentioning

confidence: 59%

Exploring Anti-Type 2 Diabetes Mellitus Mechanism of Gegen Qinlian Decoction by Network Pharmacology and Experimental Validation

Bao

Sun

et al. 2022

Disease Markers

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“…Insulin resistance and β-cell dysfunction are the two primary insulin-related abnormalities that define this form of diabetes (34,35). Insulin resistance is brought on by disruptions in a variety of cellular pathways, which lower the insulin sensitivity of cells in peripheral tissues, including muscle, the liver, and adipose tissue.…”

Section: Type 2 Diabetes Mellitus T2dmmentioning

confidence: 99%

An Efficacy of Anti-hyperglycemic Agents (Nigella sativa) in Blood, Body Weight and Glucose levels of Diabetes Mellitus Rats: A Comprehensive Review

Ofori¹,

Yeboah²,

Amissah-Reynolds³

2023

Int J Health Sci Res

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There is a large global trend toward the use of medicinal herbs for the treatment of diabetes, and many people employ complementary and alternative medicine. The objective of the study was to provide comprehensive information on the effects of Nigella sativa on lipid profile, glucose level, and body weight in diabetic-induced rats. This is a comprehensive review carried out through PubMed, Science Direct, Google scholar and Springer and other Scientific Electronic Library Online databases, using the keywords; “N. sativa”, “black seed”, “diabetes”, “glucose”, “lipid”, “Weight”, and “insulin” associated with the Boolean operator “AND”. At first, 241 articles from 2000 to 2022 were discovered. Following the application of the specified inclusion and exclusion criteria, 21 papers were still available, and 15 underwent complete reading, after which all were included in the study. According to their findings, N. sativa has a variety of possible mechanisms for controlling hyperglycemia and abnormal lipid profiles, including its antioxidant properties and influences on insulin secretion, glucose absorption and body weight. However, further clinical studies are required to determine the N. sativa therapeutic benefits, as well as the kind and dose that work best for managing diabetes and its consequences. Key words: Diabetes Mellitus, glucose, Lipid, hyperglycemia, Metabolism, Cumin seeds

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“…He et al initiated a controlled study to explore the pathogenesis of type 2 diabetes mellitus (T2DM) intestinal damp-heat syndrome (IDHS) from the perspective of exosomal miRNA, which enrolled 24 patients with IDHS of T2DM and 24 healthy people ( He et al, 2021 ). According to the results of chIp-sequencing and qPCR verification, exosomal hsa-miR-9-5p, hsa-miR-150-5p, and hsa-miR-216b-5p were significantly increased in the IDHS patients compared with the healthy group.…”

Section: Interplay Between Exosomes and Tcm Syndromesmentioning

confidence: 99%

“…Moreover, exosomes may be the potential delivery carriers for compounds isolated from TCM ( Dong M. et al, 2021a ). TCM syndromes are closely associated with exosomes ( He et al, 2021 ). These results demonstrate that exosomes may be a novel insight into TCM.…”

Section: Introductionmentioning

confidence: 99%

Exosomes: A novel insight into traditional Chinese medicine

Zhao

Liang

et al. 2022

Front. Pharmacol.

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Exosomes are small extracellular vesicles and play an essential role in the mediation of intercellular communication both in health and disease. Traditional Chinese medicine (TCM) has historically been used to maintain human health and treat various diseases up till today. The interplay between exosomes and TCM has attracted researchers’ growing attention. By integrating the available evidence, TCM formulas and compounds isolated from TCM as exosome modulators have beneficial effects on multiple disorders, such as tumors, kidney diseases, and hepatic disease, which may associate with inhibiting cells proliferation, anti-inflammation, anti-oxidation, and attenuating fibrosis. Exosomes, a natural delivery system, are essential in delivering compounds isolated from TCM to target cells or tissues. Moreover, exosomes may be the potential biomarkers for TCM syndromes, providing strategies for TCM treatment. These findings may provide a novel insight into TCM from exosomes and serve as evidence for better understanding and development of TCM.

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Exploring the pathogenesis of type 2 diabetes mellitus intestinal damp-heat syndrome and the therapeutic effect of Gegen Qinlian Decoction from the perspective of exosomal miRNA

Cited by 9 publications

References 77 publications

Exploring Anti-Type 2 Diabetes Mellitus Mechanism of Gegen Qinlian Decoction by Network Pharmacology and Experimental Validation

Exploring Anti-Type 2 Diabetes Mellitus Mechanism of Gegen Qinlian Decoction by Network Pharmacology and Experimental Validation

An Efficacy of Anti-hyperglycemic Agents (Nigella sativa) in Blood, Body Weight and Glucose levels of Diabetes Mellitus Rats: A Comprehensive Review

Exosomes: A novel insight into traditional Chinese medicine

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