2012
DOI: 10.1016/j.jprot.2012.07.025
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Exploring the pan-surfome of Streptococcus suis: Looking for common protein antigens

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Cited by 31 publications
(36 citation statements)
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“…Many of these were also identified in a high proportion of adult clinical isolates (22). Our results show the enrichment of LPXTG-cell wall proteins when compared with their predicted figures from the genome, as already demonstrated in previous works for this and other pathogens (20,21,48,49,(51)(52)(53)(54). We decided to include also some cytoplasmic proteins, as for some of them their surface localization and immunogenicity/protective activity, are described, like Eno (55,56) or GAPDH (57).…”
Section: Figsupporting
confidence: 76%
“…Many of these were also identified in a high proportion of adult clinical isolates (22). Our results show the enrichment of LPXTG-cell wall proteins when compared with their predicted figures from the genome, as already demonstrated in previous works for this and other pathogens (20,21,48,49,(51)(52)(53)(54). We decided to include also some cytoplasmic proteins, as for some of them their surface localization and immunogenicity/protective activity, are described, like Eno (55,56) or GAPDH (57).…”
Section: Figsupporting
confidence: 76%
“…Several proteomic analyses have been carried out on the pneumococcus, targeting either the membrane fraction [31][33] or the cell wall-attached proteins [34], [35], but these biochemical fractionation-based methods have several limitations: they are relatively slow, membrane proteins are not always well resolved in polyacrylamide gels and topology information is lost [19], [36]. The “shaving” approach has become a powerful way to identify the set of surface proteins expressed on a given organism (the “surfome”), most of which are normally highly immunogenic as shown by different immunochemical techniques [15], [16], [37], and provides new candidates that elicit protective activity against infection [38]. We have previously set up this strategy in the pneumococcus [19], showing that the procedure enables to define the “pan-surfome” of a collection of clinical isolates, in order to select common proteins to all or most strains [37], [39].…”
Section: Resultsmentioning
confidence: 99%
“…The “shaving” approach has become a powerful way to identify the set of surface proteins expressed on a given organism (the “surfome”), most of which are normally highly immunogenic as shown by different immunochemical techniques [15], [16], [37], and provides new candidates that elicit protective activity against infection [38]. We have previously set up this strategy in the pneumococcus [19], showing that the procedure enables to define the “pan-surfome” of a collection of clinical isolates, in order to select common proteins to all or most strains [37], [39].…”
Section: Resultsmentioning
confidence: 99%
“…In 2004, a cell-associated DNase (112 kDa), encoded by the ssnA gene, has been identified in S. suis serotype 2 [15]. The recent pan-surfome analysis performed on S. suis and which concluded that the cell-associated DNase may represent one of the best vaccine candidates has revived the interest for this protein [16]. More specifically, it was found that the S. suis DNase is largely distributed among serotypes, and is highly immunogenic and accessible to antibodies [16].…”
Section: Introductionmentioning
confidence: 99%
“…The recent pan-surfome analysis performed on S. suis and which concluded that the cell-associated DNase may represent one of the best vaccine candidates has revived the interest for this protein [16]. More specifically, it was found that the S. suis DNase is largely distributed among serotypes, and is highly immunogenic and accessible to antibodies [16]. In addition, de Buhr et al [17] recently reported that S. suis DNase is involved in the degradation and escaping of neutrophil extracellular traps.…”
Section: Introductionmentioning
confidence: 99%