Exploring the oncogenic and tumor-suppressive roles of Circ-ADAM9 in cancer

Hussain, Md Sadique; Moglad, Ehssan; Bansal, Pooja; Kaur, Harpreet; Deorari, Mahamedha; almalki, Waleed Hassan; Kazmi, Imran; Alzarea, Sami I.; Singh, Mahaveer; Kukreti, Neelima

doi:10.1016/j.prp.2024.155257

Cited by 7 publications

(2 citation statements)

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“…However, few studies have focused on the regulatory effect of circRNAs on the radiosensitivity of ESCC. On the basis of our previous work [ 23 ], the expression profiles of radioresistant KYSE150R and radiosensitive KYSE150 cells were detected by AgilenthumancircRNA microarray. It was found that compared with KYSE150 cells, there were 74 circRNAs with more than 2-fold difference in radiation-resistant KYSE150R group, including 57 up-regulated circRNAs expression and 17 down-regulated circRNAs expression.…”

Section: Introductionmentioning

confidence: 99%

“…Many studies have shown that circRNA has multiple functions in regulating tumorigenesis and development: (a) circRNA as miRNA sponge [ 24 ]; (b) circRNA binding to protein [ 25 ]; (c) circRNA translation into protein [ 26 ]; (d) transcriptional regulatory factor [ 27 ]. we mainly predicted circRNA spongified miRNA so as to affect the radiosensitivity of ESCC in our previous work [ 23 ], so this study selected spongified miRNA as the related mechanism of circRNA affecting ESCC radiosensitivity. miRNAs [ 28 , 29 ] is a large class of short (~ 22nt) ncRNA, which promotes the degradation of target gene mRNA or inhibits its translation by pairing with the 3 ‘UTR (untranslated region) of the target gene.…”

Section: Introductionmentioning

confidence: 99%

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CircRNA_101491 regulated the radiation sensitivity of esophageal squamous cell carcinomas via sponging miR-125a-5p

Lin,

Huang,

Qian

et al. 2024

Radiat Oncol

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Background At present, it has been found that many patients have acquired resistance to radiotherapy, which greatly reduces the effect of radiotherapy and further affects the prognosis. CircRNAs is involved in the regulation of radiosensitivity of many kinds of tumor cells. Therefore, the main purpose of this study is to explore the regulatory effect of CircRNA_101491 on radiosensitivity of ESCC and its related mechanism. Methods We established ESCC radiation-resistant cell line (KYSE150R cell) by gradient dose method, and tested the difference of KYSE150 between KYSE150R cell and parent cell in vitro. Then, after knocking down the expression of CircRNA_101491, a series of in vitro experiments were conducted to verify the effects of CircRNA_101491 on the phenotype and radiosensitivity of KYSE150R cells, and further analyzed the related regulatory mechanism. In addition, we also used the model of transplanted tumor in nude mice to investigate the effect of CircRNA_101491 on the radiosensitivity of ESCC in vivo. Results According to a series of in vitro experiments, we confirmed that KYSE150R cells lost the epithelial phenotype and obtained interstitial cell-like phenotype, and found that CircRNA_101491 was highly expressed in KYSE150R cells. In addition, we found that knocking down the expression of CircRNA_101491 will lift the inhibition of miR-125a-5p, and then reverse the process of EMT, accelerate the process of apoptosis, thus play a role in radiosensitization. The in vivo experiment of transplanted tumor in nude mice also showed that knocking down the expression of CircRNA_101491 could enhance the radiosensitivity of ESCC. Conclusion In conclusion, we confirmed that interfering with the expression of CircRNA_101491 can relieve the inhibition of miR-125a-5p, thus reverse the process of interstitial phenotype, accelerate the process of apoptosis, and enhance the radiosensitivity of ESCC.

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Section: Introductionmentioning

confidence: 99%