Exploring the Mitochondrial Proteome of the Ciliate Protozoon Tetrahymena thermophila: Direct Analysis by Tandem Mass Spectrometry

Mitochondrial Evolution

“…At least 13 novel, "ciliate-specific" proteins identified during MS analysis of mitochondria from Tetrahymena pyriformis (Smith et al 2007) were subsequently found as components of the purified mitochondrial F 1 F 0 -ATP synthase (complex V) of this protist (Nina et al 2010). These observations highlight an emerging theme of taxon-specific retooling of mitochondrial complexes such as electron transport chain assemblies and ribosomes, only the core a-Proteobacteria-like components of which appear to have been contributed by the proto-mitochondrion.…”

Differential remodelling of peroxisome function underpins the environmental and metabolic adaptability of diplonemids and kinetoplastids

“…The glucose concentration of the culture supernatant was measured using a Glucose-Oxidase assay kit (Sigma-Aldrich). Amino acid consumption by cells was evaluated by the increase of NH 4 þ in the culture supernatant using the Ammonia assay kit (Sigma-Aldrich).…”

Subcomplexes of Ancestral Respiratory Complex I Subunits Rapidly Turn Over in Vivo as Productive Assembly Intermediates in Arabidopsis*

“…Aside from Arabidopsis, direct evidence for the presence of both ␥-CAs and the 20.9-kDa subunit in mitochondrial CI is available for the green alga, Chlamydomonas reinhardtii (32), as well as Acanthamoeba castellanii (33). Various of these components have also been identified in proteomic screens of purified mitochondria from additional nonphotosynthetic protists, including Tetrahymena thermophila (34), Dictyostelium discoideum (35), and Trypanosoma brucei (36,37) (Table 1). Evidently, the subunits of the subcomplex described here in Arabidopsis are widely conserved and coincident in CI across eukaryotes.…”