Exploring the metabolic features of purple non-sulfur bacteria for waste carbon utilization and single-cell protein synthesis

Rashid, Naim; Onwusogh, Udeogu; Mackey, Hamish R.

doi:10.1007/s13399-022-03273-8

Cited by 6 publications

(2 citation statements)

References 131 publications

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“…The maximum protein percentage of 34% is lower compared to studies conducted under photoorganoheterotrophic conditions. Indeed, previous studies reported that the protein content of PPB can vary between 45% and 75% [19]. This result was predictable, since, in this study, the process was conducted under photo fermentative conditions instead of photoorganoheterotrophy.…”

Section: Resultsmentioning

confidence: 57%

Valorization of Purple Phototrophic Bacteria Biomass Resulting from Photo Fermentation Aimed at Biohydrogen Production

Policastro,

Cesaro,

Fabbricino

2024

Molecules

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This study evaluated the feasibility of contextually producing hydrogen, microbial proteins, and polyhydroxybutyrate (PHB) using a mixed culture of purple phototrophic bacteria biomass under photo fermentative conditions. To this end, three consecutive batch tests were conducted to analyze the biomass growth curve and to explore the potential for optimizing the production process. Experimental findings indicated that inoculating reactors with microorganisms from the exponential growth phase reduced the duration of the process. Furthermore, the most effective approach for simultaneous hydrogen production and the valorization of microbial biomass was found when conducting the process during the exponential growth phase of the biomass. At this stage, achieved after 3 days of fermentation, the productivities of hydrogen, PHB, and microbial proteins were measured at 63.63 L/m3 d, 0.049 kg/m3 d, and 0.045 kg/m3 d, respectively. The biomass composition comprised a total intracellular compound percentage of 56%, with 27% representing PHB and 29% representing proteins. Under these conditions, the estimated daily revenue was maximized, amounting to 0.6 $/m3 d.

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Section: Resultsmentioning

confidence: 57%

Valorization of Purple Phototrophic Bacteria Biomass Resulting from Photo Fermentation Aimed at Biohydrogen Production

Policastro,

Cesaro,

Fabbricino

2024

Molecules

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“…In this perspective, biological treatments are desirable due to relatively low energy inputs. Recently, anaerobic treatment using PNSB has received considerable research attention [2][3][4]. They are considered favorable due to their unique metabolic features.…”

Section: Introductionmentioning

confidence: 99%

An integrated approach for treatment and biomass resource recovery from juice industry wastewater using purple non-sulfur bacteria

2023

Sustainable Processes and Clean Energy Transition

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Abstract. This study aims to treat juice industry wastewater and recover resources using purple non-sulfur bacteria (PNSB). Simulated juice wastewater was prepared and used as a feedstock for PNSB. Carbon sources, including sucrose, fructose, glucose, and tris-Na-citrate, were added to different bottles separately and inoculated with a mixed culture of PNSB. Tris-Na-citrate showed higher growth and biomass production (650±70 mg/L) than the other carbon sources. The PNSB growth was associated with the pH change; tris-Na-citrate showed different pH change behavior than the other carbon sources, i.e., it showed a lesser decrease in the medium pH than the others. The effect of substrate (using glucose) concentration on PNSB biomass production was determined in the following experiment. A substrate concentration of 400 mg/L (as COD) showed the highest growth, whereas 800 mg/L (as COD) returned the highest biomass production (776±1 mg/L). Total organic carbon removal (TOC) increased with an increase in substrate concentration. Inorganic carbon removal initially decreased but then increased over the growth period; however, it was also directly correlated with initial substrate concentration. Lastly, the effect of pH on TOC, IC, and COD removal was investigated. A pH of 6.0 or above (up to 8.0) was required to remove organic and inorganic carbon.

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Simultaneous treatment of fruit juice industry wastewater and single-cell protein synthesis using purple non-sulfur bacteria

Rashid

Abdelnabi

Vincent

et al. 2022

Biomass Conv. Bioref.

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Fruit juice industry wastewater is characterized by high chemical oxygen demand (COD) and low pH, which requires extensive energy if treated aerobically and can adversely affect aquatic organisms if discharged into water bodies without proper treatment. In this study, purple non-sulfur bacteria (PNSB) were employed to recover carbon through photoanaerobic treatment in the form of single-cell protein (SCP) suitable for aquaculture and rich in carotenoid and bacteriochlorophyll photopigments. The efficacy of PNSB was investigated using two types of wastewaters, citrus juice wastewater (CJW), and mixed juice wastewater (MJW) as substrates at different pH conditions (5.0–8.0). pH 8.0 showed the highest COD removal in CJW (89 ± 0.1%) and pH 7.0 in MJW (94 ± 1%), respectively. The biomass yields were 0.493 g-VSS·g-COD−1 and 0.397 g-VSS·g-COD−1 for CJW and MJW. SCP contents in CJW and MJW were 48 ± 1% and 40 ± 3%, respectively. Pigment analysis revealed that PNSB biomass contained carotenoids and bacteriochlorophyll. Carotenoids in CJW and MJW were 1354 ± 64 µg·g−1 and 1558 ± 218 µg·g−1 of biomass; bacteriochlorophyll contents were 2097 ± 110 µg·g−1 and 3100 ± 614 µg·g−1 of biomass, respectively. The microbial population showed diversity among the two substrates and pH conditions, with PNSB comprising potentially up to 51% of microbes in the culture. The results demonstrate that PNSB could be used as potential mediators to treat fruit juice wastewater, and produce SCP, and photopigments simultaneously.

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Exploring the metabolic features of purple non-sulfur bacteria for waste carbon utilization and single-cell protein synthesis

Cited by 6 publications

References 131 publications

Valorization of Purple Phototrophic Bacteria Biomass Resulting from Photo Fermentation Aimed at Biohydrogen Production

Valorization of Purple Phototrophic Bacteria Biomass Resulting from Photo Fermentation Aimed at Biohydrogen Production

An integrated approach for treatment and biomass resource recovery from juice industry wastewater using purple non-sulfur bacteria

Simultaneous treatment of fruit juice industry wastewater and single-cell protein synthesis using purple non-sulfur bacteria

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