Exploring the metabolic biomarkers and pathway changes in crucian under carbonate alkalinity exposure using high-throughput metabolomics analysis based on UPLC-ESI-QTOF-MS

Sun, Yanchun; Han, Shicheng; Yao, Ming-zhu; Liu, Hongbai; Wang, Yumei

doi:10.1039/c9ra08090b

Cited by 18 publications

(6 citation statements)

References 132 publications

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“…After using the Pareto method to standardize the data, it was determined that some metabolic pathways in the blood involved in phenylalanine, tyrosine and tryptophan biosyntheses, phenylalanine metabolism, valine, leucine, and isoleucine biosyntheses, sulfur metabolism, tricarboxylic acid (TCA) cycle, and nicotinate and nicotinamide metabolism were associated with the cardiovascular toxicity effect of acrylamide. The metabolic pathway influence values are listed in Table S1, where a metabolic pathway with an influence value greater than or equal to 0.1 can be selected as a potential key metabolic pathway for acrylamide toxicity …”

Section: Resultsmentioning

confidence: 99%

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Unraveling the Serum Metabolomic Profile of Acrylamide-Induced Cardiovascular Toxicity

Wang

Chen

et al. 2021

J. Agric. Food Chem.

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Acrylamide has been reported as an important dietary risk factor from carbohydrate-rich processing food. However, systemic biological effects on the serum metabolomics induced by acrylamide have poorly been understood. In the present study, we evaluated the metabolic profiles in a rat serum after exposure to acrylamide using ultrahigh-performance liquid chromatography combined with quadrupole-orbitrap high-resolution mass spectrometry. The serum biochemical parameters of the treated and control groups were also determined using an automatic biochemical analyzer. Compared with the control group, 10 metabolites were significantly upregulated, including citric acid, D-(−)-fructose, gluconic acid, L-ascorbic acid 2-sulfate, 2-hydroxycinnamic acid, valine, L-phenylalanine, prolylleucine, succinic acid, and cholic acid, while 5 metabolites were significantly downregulated, including 3-hydroxybutyric acid, 4-oxoproline, 2,6-xylidine, 4-phenyl-3-buten-2-one, and N-ethyl-N-methylcathinone in the serum of 4-weekold rats exposed to acrylamide in the high-dose group (all P < 0.05). Importantly, acrylamide exposure affected metabolites mainly involved in the citrate cycle, valine, leucine, and isoleucine biosyntheses, phenylalanine, tyrosine and tryptophan biosyntheses, and pyruvate metabolism. These results suggested that exposure to acrylamide in rats exhibited marked systemic metabolic changes and affected the cardiovascular system. This study will provide a theoretical basis for exploring the toxic mechanism and will contribute to the diagnosis and prevention of acrylamide-induced cardiovascular toxicity.

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Section: Resultsmentioning

confidence: 99%

“…The metabolic pathway influence values are listed in Table S1, where a metabolic pathway with an influence value greater than or equal to 0.1 can be selected as a potential key metabolic pathway for acrylamide toxicity. 25…”

Section: Analysis Of Metabolic Pathwaysmentioning

confidence: 99%

Unraveling the Serum Metabolomic Profile of Acrylamide-Induced Cardiovascular Toxicity

Wang

Chen

et al. 2021

J. Agric. Food Chem.

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“…It has been widely applied in unraveling underlying mechanisms of physiological processes and abiotic stress responses in aquatic organisms [ 23 ]. Previous studies have reported the metabolic variation characteristics of the crucian carp gill and liver in response to saline-alkali stress by using metabolomics technology [ 21 , 24 , 25 ]. However, there is little information concerning the mechanism underlying the carbonate alkaline stress-induced toxic effects and changes in metabolic profile.…”

Section: Introductionmentioning

confidence: 99%

Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

et al. 2023

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The salinization of the water environment caused by human activities and global warming has increased which has brought great survival challenges to aquatic animals. Crucian carp (Carassius auratus) is an essential freshwater economic fish with superior adaptability to saline-alkali water. However, the physiological regulation mechanism of crucian carp adapting to saline-alkali stress remains still unclear. In this study, crucian carp were exposed to freshwater or 20, 40, and 60 mmol/L NaHCO3 water environments for 30 days, the effects of saline-alkali stress on the kidney were evaluated by histopathology, biochemical assays and metabolomics analysis from renal function, antioxidant capacity and metabolites level. Our results showed different degrees of kidney damage at different exposure concentrations, which were characterized by glomerular atrophy and swelling, renal tubular degranulation, obstruction and degeneration, renal interstitial edema, renal cell proliferation and necrosis. Saline-alkali stress could change the levels of several physiological parameters with renal function and antioxidant capacity, including creatinine (CREA), urea nitrogen (BUN), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) and malondialdehyde (MDA). In addition, metabolomics analysis showed that differential metabolites (DMs) were involved in various metabolic pathways, including phenylalanine, tyrosine, and tryptophan biosynthesis, aminoacyl-tRNA biosynthesis, purine metabolism, glycerophospholipid metabolism, sphingolipid metabolism, glycolysis/gluconeogenesis and the TCA cycle. In general, our study revealed that saline-alkaline stress could cause significant changes in renal function and metabolic profiles, and induce severe damage in the crucian carp kidney through destroying the anti-oxidant system and energy homeostasis, inhibiting protein and amino acid catabolism, as well as disordering purine metabolism and lipid metabolism. This study could contribute to a deeper understanding the adverse effects of saline-alkali stress on crucian carp kidney and the regulatory mechanism in the crucian carp of saline-alkali adaptation at the metabolic level.

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“…It has been widely used to study the abstruse metabolic mechanisms of fish in response to changes in endogenous and exogenous factors [ 17 ]. Sun et al [ 18 ] utilized UPLC-QTOF/MS metabolomics technology to explore the changes in biomarkers and pathways of crucian carp ( Carassius auratus ) under carbonate alkalinity exposure to reveal the molecular and physiological mechanisms of fish salt tolerance. Ladisa et al [ 19 ] employed UHPLC-MS technology to investigate the seasonal metabolic changes and energy distribution associated with the growth and reproductive stages of the liver of male goldfish ( Carassius auratus ).…”

Section: Introductionmentioning

confidence: 99%

UPLC-QTOF/MS Metabolomics and Biochemical Assays Reveal Changes in Hepatic Nutrition and Energy Metabolism during Sexual Maturation in Female Rainbow Trout (Oncorhynchus mykiss)

Ding

Liu²,

Meng³

et al. 2022

Biology

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Mobilization and repartition of nutrients and energy are prerequisites for the normal sexual maturity of broodstock. However, there are few studies on the mechanisms of hepatic nutrients and energy metabolism during sexual maturation in female rainbow trout (Oncorhynchus mykiss). This study investigated hepatic metabolite changes and explored the potential nutritional regulation mechanism between mature and immature female rainbow trout by combining UPLC-QTOF/MS metabolomics and biochemical assays. It was observed that hepatic biochemical assays differed considerably between the two groups, such as glucose, triglycerides, hexokinase, lipase, and aspartate aminotransferase. Liver metabolomics showed that various differential metabolites involved in amino acid and lipid metabolism markedly increased, suggesting the enhancement of lipid metabolism and amino acid anabolism in the liver provides the necessary material basis for ovarian development. Meanwhile, glycogen catabolism and glycolysis hold the key to maintaining organismal energy homeostasis with normal sexual maturation of female rainbow trout. Overall, the results from this study suggested that the liver undergoes drastic reprogramming of the metabolic profile in response to mobilization and repartition of nutrients and energy during the sexual maturation of female rainbow trout. This study further deepened the understanding of the reproductive biology of rainbow trout, and provided the theoretical basis and practical ramifications for nutritional requirements of breeding high-quality broodstock in the artificial propagation of rainbow trout.

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Exploring the metabolic biomarkers and pathway changes in crucian under carbonate alkalinity exposure using high-throughput metabolomics analysis based on UPLC-ESI-QTOF-MS

Abstract: We explore the metabolic biomarker and pathway changes accompanying the adaptive evolution of crucian subjected to carbonate alkalinity exposure, using UPLC-ESI-QTOF-MS, in order to understand the molecular physiological mechanisms of saline–alkali tolerance.

Cited by 18 publications

References 132 publications

Unraveling the Serum Metabolomic Profile of Acrylamide-Induced Cardiovascular Toxicity

Unraveling the Serum Metabolomic Profile of Acrylamide-Induced Cardiovascular Toxicity

Effects of Saline-Alkaline Stress on Metabolome, Biochemical Parameters, and Histopathology in the Kidney of Crucian Carp (Carassius auratus)

UPLC-QTOF/MS Metabolomics and Biochemical Assays Reveal Changes in Hepatic Nutrition and Energy Metabolism during Sexual Maturation in Female Rainbow Trout (Oncorhynchus mykiss)

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