Exploring the mechanism of Yishen Daluo decoction in the treatment of multiple sclerosis based on network pharmacology and in vitro experiments

Cheng, Shuo; Zhang, Ce; Cai, Qing-Yuan; Wang, Xinghua; Liu, Zhaoheng; Peng, Wei; Wang, Xu; Tan, Yan; Qian, Hua

doi:10.1016/j.jtcms.2023.03.002

Cited by 2 publications

(2 citation statements)

References 27 publications

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“…It appears that the PI3K/Akt/mTOR pathway negatively regulates autophagy as seen in WD [ 11 , 39 ]. This effect has been documented to be significantly inhibited by TCM in vitro experiments [ 40 , 41 ]. Similarly, this study revealed that GDFMD markedly reduced LC3 and Beclin-1 expressions while raising p62, p-PI3K, p-Akt, and p-mTOR expressions.…”

Section: Discussionmentioning

confidence: 99%

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Tang

Zhao

Zhang

et al. 2023

Mediators of Inflammation

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Background. Gandoufumu decoction (GDFMD) is a traditional Chinese medicine that has been widely used to treat Wilson’s disease (WD) liver damage patients. However, its specific molecular mechanism currently remains unclear. Autophagy as a key contributor to WD liver damage has been intensely researched in the recent years. Therefore, the aim of this present study is to explore the effect of GDFMD on autophagy in WD liver damage, and the final purpose is to provide scientific evidence for GDFMD treatment in WD liver damage. Methods. The molecular mechanisms and autophagy-related pathways of GDFMD in the treatment of WD liver damage were predicted using network pharmacology. Copper assay kit was used to determine copper content in serum. Enzyme-linked immunosorbent assay (ELISA) was utilized to quantify serum levels of liver enzymes and oxidative stress-related indicators. Hematoxylin-eosin (HE), Masson, and Sirius red staining were used for the characterization of liver pathological changes. Transmission electron microscopy, immunofluorescence, and Western blot analyses were used to evaluate autophagy activity. The impact of the GDFMD on typical autophagy-related pathway (PI3K/Akt/mTOR pathway) molecules was also assessed via Western blot analysis. Results. GDFMD effectively attenuated serum liver enzymes, oxidative stress, autophagy, and degree of hepatic histopathological impairment and reduced serum copper content. Through network pharmacological approaches, PI3K/Akt/mTOR pathway was identified as the typical autophagy-related pathway of GDFMD in the treatment of WD liver damage. Treatment with GDFMD activated the PI3K/Akt/mTOR pathway, an effect that was able to be counteracted by LY294002, a PI3K antagonist or Rapa (rapamycin), an autophagy inducer. Conclusions. GDFMD imparted therapeutic effects on WD through autophagy suppression by acting through the PI3K/Akt/mTOR pathway.

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Section: Discussionmentioning

confidence: 99%

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Tang

Zhao

Zhang

et al. 2023

Mediators of Inflammation

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“…It is a disorder of CNS in which there is an inflammation in the myelin sheath of axons. Mitochondrial dysfunctioning is associated with the pathogenesis of MS which has been revealed in recent research (Cheng et al, 2023).…”

Section: Multiple Sclerosismentioning

confidence: 98%

Prominent Medicinal Plants for the Treatment of Neurodegenerative Disorders

Sajid,

Abbas,

Khan

et al. 2023

Complementary and Alternative Medicine: One Health Perspective

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The phrase "neurodegenerative diseases" refers to conditions that cause a major loss of neurons, both structurally and functionally. Neurodegenerative diseases are serious life-threatening in which neural cells in the brain stop working or die. One of the most recently discovered pathogenic processes in the genesis of neurological illnesses is mitochondrial dysfunction. Mitochondria play a significant role in various cellular processes such as the production of ATP, intracellular calcium, and generation of reactive oxygen species and improper function leads to diseases. Oxidative stress and aging are two other prevalent etiologies of neurological diseases. Several side effects have emerged as a result of current treatment methods and the progressive nature of illnesses, prompting patients to switch to other medications. The helpful impact of medicinal plants in these situations has been attributed to their demonstration through several cellular and molecular processes. Traditional plants have a few neuroprotective mechanisms: they reduce inflammatory responses, regulate pro-inflammatory cytokines' functional aspects such as tumor growth, and enhance antioxidant qualities. Plant prevention strategies for these diseases heavily rely on variations in transcription and transduction pathways. Some of the therapeutic herbs for preventing neurological disorders are Panax ginseng, Curcuma longa, Zingiber officinale, Ginkgo biloba, and Utrica diocia, etc., have shown antioxidant as well as anti-inflammatory effects and also show a promising tool for treating neurodegenerative diseases such as Alzheimer's, Parkinson's, Huntington's, multiple sclerosis, and epilepsy. Medicinal plants contain various phytochemical constituents that improve oxidative stress, reduce mitochondrial damage, and protect against brain damage. In short, use of whole plants or parts of medicinal plants from roots to seeds extracted chemicals are used to prevent neurodegenerative illnesses.

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Exploring the mechanism of Yishen Daluo decoction in the treatment of multiple sclerosis based on network pharmacology and in vitro experiments

Cited by 2 publications

References 27 publications

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Discussion on the Mechanism of Gandoufumu Decoction Attenuates Liver Damage of Wilson’s Disease by Inhibiting Autophagy through the PI3K/Akt/mTOR Pathway Based on Network Pharmacology and Experimental Verification

Prominent Medicinal Plants for the Treatment of Neurodegenerative Disorders

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