Exploring the mechanism of Shengmai Yin for coronary heart disease based on systematic pharmacology and chemoinformatics

Abstract

Objective: To explore the mechanism of Shengmai Yin (SMY) for coronary heart disease (CHD) by systemic pharmacology and chemoinformatics. Methods: Traditional Chinese Medicine Systems Pharmacology Database (TCMSP), traditional Chinese medicine integrative database (TCMID) and the traditional Chinese medicine (TCM) Database@Taiwan were used to screen and predict the bioactive components of SMY. Pharmmapper were utilized to predict the potential targets of SMY, the TCMSP was utilized to obtain the known targets of SMY. The Genecards and OMIM database were utilized to collect CHD genes. Cytoscape was then used for network construction and analysis, and DAVID was used for Gene Ontology (GO) and pathway enrichment analysis. After that, animal experiments were then performed to further validate the results of systemic pharmacology and chemoinformatics. Results: Three major networks were constructed: (1) CHD genes’ protein–protein interaction (PPI) network; (2) SMY–CHD PPI network; (3) SMY known target–CHD PPI network. The other networks are minor networks generated by analyzing the three major networks. Experimental results showed that compared with the model group, the Shengmai injection (SMI) can reduce the myocardial injury score and the activities of serum aspartate aminoconvertase (AST), CK and lactate dehydrogenase (LDH) in rats (P<0.05), and reduce serum lipid peroxide (LPO) content and increase serum superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in myocardial infarction rats (P<0.05). SMI can also decrease the expression of MMP-9 mRNA and increase that of TIMP-1 mRNA (P<0.01). Conclusion: SMY may regulate the signaling pathways (such as PPAR, FoxO, VEGF signaling), biological processes (such as angiogenesis, blood pressure formation, inflammatory response) and targets (such as AKT1, EGFR, MAPK1) so as to play a therapeutic role in CHD.

Eight Trigrams Boxing Combined with Wenyang Huoxue Recipe Improves Cardiopulmonary Motor Function and the Quality of Life of Patients with Coronary Heart Disease

“…Traditional Chinese medicine (TCM) emphasizes the importance of health maintenance and rehabilitation in cardiac rehabilitation. 14,15 Yang deficiency blood stasis syndrome is the basic pathogenesis of patients following PCI. Our center created a Wenyang Huoxue recipe to treat patients with Yang deficiency blood stasis syndrome after coronary artery PCI and conducted a systematic study on cardiac rehabilitation of integrated traditional Chinese and Western medicine.…”

The Protective Effect of Sheng Mai Yin on Diabetic Cardiomyopathy via NLRP3/Caspase-1 Pathway

“…In this study, we found that SMY had the same effect as Met in improving cardiac function damage, which led us to believe that SMY can protect the heart from diabetes. SMY, a traditional Chinese medicine, is commonly used in the clinic to treat cardiac insufficiency, coronary heart disease, and heart failure [ 8 , 9 , 30 ]. SMY could restore the cardiac function of CHF rats, reduce serum biochemical indexes, reduce cardiac tissue damage, and reduce the expression levels of ALOX15 and CYP1A2, which may be related to the linoleic acid metabolic pathway [ 10 ].…”

A Quantitative Serum Proteomic Analysis Helps to Explore the Comprehensive Mechanism and Identify Serum Biomarkers of Shengmai Injection’s Effect on Isoproterenol-Induced Myocardial Ischemia in Rats

“…Some pharmacological studies reported SMI’s effects of anti-inflammatory, anti-oxidation, and suppression of cell apoptosis through pathways such as caspase-3, mTOR, JNK, AKT, ERK1/2, and AMPK ( Li et al, 2019 ; Zhu et al, 2019 ; Cao et al, 2020 ). Recently, Jiang et al ( Jiang et al, 2020 ) used systemic pharmacology and chemoinformatics to predict signaling pathways, biological processes, and targets of Shengmai Yin (including SMI) therapeutic role in coronary heart disease. Interestingly, in this serum proteome, we found 100 DEPs owing to SMI’s intervention could be mapped to 184 signaling pathways by KEGG database, and these pathways cover most of the reported.…”