Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq

Wang, Hourong; Liu, Zhifeng; Yu, Tianyuan; Zhang, Yingqi; Xu, Yajing; Yi, Jae-Seon; Guan, Qian; Liu, Di

doi:10.3389/fnins.2022.1007432

Cited by 5 publications

(4 citation statements)

References 41 publications

(34 reference statements)

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“…In recent years, research on CAT for the treatment of NP has increased year by year, with a proliferation of systematic reviews, large-sample randomized controlled trials, and original studies. [31][32][33][34] However, bibliometric analyses of the disciplinary structure, status of study, and hotspots in the area are still vacant. Therefore, in this study, we summarized the general information of this research field by bibliometrics, and analyzed the contributions and collaborations of countries, institutions, and authors to the field.…”

Section: Discussionmentioning

confidence: 99%

Research hotspots and trends of complementary and alternative therapy for neuropathic pain: A bibliometric analysis

Wang,

Sun,

Zhao

et al. 2024

Medicine

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Background: Neuropathic pain (NP) is a common type of pain in clinic. Due to the limited effect of drug treatment, many patients with NP are still troubled by this disease. In recent years, complementary and alternative therapy (CAT) has shown good efficacy in the treatment of NP. As the interest in CAT for NP continues to grow, we conducted a bibliometric study of publications on CAT treatment for NP. The aim of this study is to analyze the development overview, research hotspots and future trends in the field of CAT and NP through bibliometric methodology, so as to provide a reference for subsequent researchers. Methods: Publications on CAT in the treatment of NP from 2002 to 2022 were retrieved from the Web of Science Core Collection. Relevant countries, institutions, authors, journals, keywords, and references were analyzed bibliometrically using Microsoft Excel 2021, bibliometric platform, VOSviewer, and CiteSpace. Results: A total of 898 articles from 46 countries were published in 324 journals, and they were contributed by 4455 authors from 1102 institutions. The most influential country and institution are China (n = 445) and Kyung Hee University (n = 63), respectively. Fang JQ (n = 27) and Evidence-Based Complementary and Alternative Medicine (n = 63) are the author and journal with the most publications in this field. The clinical efficacy, molecular biological mechanisms and safety of CAT for NP are currently hot directions. Low back pain, postherpetic neuralgia, acupuncture, and herbal are the hot topics in CAT and NP in recent years. Conclusion: This study reveals the current status and hotspots of CAT for NP. The study also indicates that the effectiveness and effect mechanism of acupuncture or herbs for treating emotional problems caused by low back pain or postherpetic neuralgia may be a trend for future research.

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Section: Discussionmentioning

confidence: 99%

Research hotspots and trends of complementary and alternative therapy for neuropathic pain: A bibliometric analysis

Wang,

Sun,

Zhao

et al. 2024

Medicine

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“…Furthermore, the levels decreased after injection of TLR4 inhibitors in the model group and increased after administration of TLR4 activator in the tuina group. Wang et al (2022), using RNA-Seq, found that one-time tuina intervention could regulate TLR and NF-κB signaling pathways in minor chronic constriction injury (CCI) rats. It is suggested that tuina can reduce the expression of inflammatory factors by inhibiting the activation of TLR4/NF-κB signaling pathways, thereby achieving anti-inflammatory and analgesic effects.…”

Section: Tuina Exerts Analgesic Effects By Suppressing Peripheral Inf...mentioning

confidence: 99%

Tuina for peripherally-induced neuropathic pain: A review of analgesic mechanism

Liu

Wang

et al. 2022

Front. Neurosci.

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Peripherally-induced neuropathic pain (pNP) is a kind of NP that is common, frequent, and difficult to treat. Tuina, also known as massage and manual therapy, has been used to treat pain in China for thousands of years. It has been clinically proven to be effective in the treatment of pNP caused by cervical spondylosis, lumbar disc herniation, etc. However, its analgesic mechanism is still not clear and has been the focus of research. In this review, we summarize the existing research progress, so as to provide guidance for clinical and basic studies. The analgesic mechanism of tuina is mainly manifested in suppressing peripheral inflammation by regulating the TLR4 pathway and miRNA, modulating ion channels (such as P2X3 and piezo), inhibiting the activation of glial cells, and adjusting the brain functional alterations. Overall, tuina has an analgesic effect by acting on different levels of targets, and it is an effective therapy for the treatment of pNP. It is necessary to continue to study the mechanism of tuina analgesia.

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“…The method is simple, sensitive, accurate and can be used for the detection of amino acids and other neurotransmitters. 31 Previous studies of our research group Wang HR, etc., 32 the minor chronic constriction injury (minor CCI) rats were used as a model to simulate the clinical peripheral nerve injury research, found that the therapy of three manipulations and three acupoints instant can achieve the analgesic effect. To test the scientific hypothesis of how tuina achieves immediate pain relief, in this study, metabolomics was used to detect the changes of relevant neurotransmitters in the pain transmission pathway of minor CCI model rats, so as to explore the analgesic initiation mechanism of tuina treatment instant of peripheral nerve injury.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Mechanism of Immediate Analgesia Induced by Tuina Intervention on Minor Chronic Constriction Injury in Rats Using LC-MS

Sachula,

Yang,

et al. 2024

JPR

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This study aimed to investigate changes in metabolomic expression in the spinal dorsal horn (SDH) and thalamus during a Tuina session, aiming to elucidate the mechanism of immediate analgesia. Methods: The rats were randomly divided into three groups: the Sham group, the Model group, and the Tuina group. A minor chronic constriction injury (minor CCI) model was established in both the Model group and the Tuina group. The therapeutic effect of Tuina was determined using the mechanical withdrawal threshold (MWT) and thermal withdrawal latency (TWL) tests. Differential metabolites of the SDH and thalamus were detected using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Bioinformatic analysis was performed using CV, PCA, Venn, and KEGG. Results: The therapeutic effect of MWT and TWL after instant Tuina intervention was significant. The therapeutic effect of Tuina instant was significantly better compared to the Model group. In the Veen analysis, it was found that Tuina instantly regulates 10 differential metabolites in the SDH and 5 differential metabolites in the thalamus. In the KEGG enrichment analysis, we found that differential metabolites were enriched in 43 pathways in the thalamus and 70 pathways in the SDH. Conclusion: Tuina therapy may have analgesic effects by metabolizing neurotransmitters such as 2-Picolinic Acid, 5-Hydroxy-Tryptophan Glutathione Betaine-aldehyde-chloride Leucine Lysine Methionine Sarcosine Succinic Acid Histidine Acetylcholine and 5-Hydroxyindoleacetic Acid through the cAMP pathway. It also affects pathways of neurodegeneration-multiple diseases, butanoate metabolism, tyrosine metabolism.

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Exploring the mechanism of immediate analgesic effect of 1-time tuina intervention in minor chronic constriction injury rats using RNA-seq

Cited by 5 publications

References 41 publications

Research hotspots and trends of complementary and alternative therapy for neuropathic pain: A bibliometric analysis

Research hotspots and trends of complementary and alternative therapy for neuropathic pain: A bibliometric analysis

Tuina for peripherally-induced neuropathic pain: A review of analgesic mechanism

Exploring the Mechanism of Immediate Analgesia Induced by Tuina Intervention on Minor Chronic Constriction Injury in Rats Using LC-MS

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