Exploring the interaction mechanism between antagonist and the jasmonate receptor complex by molecular dynamics simulation

Cui, Mengqi; Zhang, Kun; Wu, Ruihan; Du, Juan

doi:10.1007/s10822-022-00441-w

Cited by 6 publications

(2 citation statements)

References 57 publications

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“…7 ). In this MD simulation, we used inositol octakisphosphate (InsP 8 ) instead of inositol pentakisphosphate (InsP 5 ) because previous reports showed that InsP 8 also formed stable complex with COI1- 2 -JAZ instead of phosphates (PO 4 ) 37 , 38 . In planta At JAZ9-selectivity of 2e was tested by using a GUS reporter assay using A. thaliana reporter lines, 35S: JAZ1-GUS , 35S: JAZ9-GUS , 35S: JAZ10-GUS , and 35S: JAZ12-GUS (Fig.…”

Section: Resultsmentioning

confidence: 99%

Subtype-selective agonists of plant hormone co-receptor COI1-JAZs identified from the stereoisomers of coronatine

et al. 2023

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Severe genetic redundancy is particularly clear in gene families encoding plant hormone receptors, each subtype sharing redundant and specific functions. Genetic redundancy of receptor family members represents a major challenge for the functional dissection of each receptor subtype. A paradigmatic example is the perception of the hormone (+)-7-iso-jasmonoyl-L-isoleucine, perceived by several COI1-JAZ complexes; the specific role of each receptor subtype still remains elusive. Subtype-selective agonists of the receptor are valuable tools for analyzing the responses regulated by individual receptor subtypes. We constructed a stereoisomer library consisting of all stereochemical isomers of coronatine (COR), a mimic of the plant hormone (+)-7-iso-jasmonoyl-L-isoleucine, to identify subtype-selective agonists for COI1-JAZ co-receptors in Arabidopsis thaliana and Solanum lycopersicum. An agonist selective for the Arabidopsis COI1-JAZ9 co-receptor efficiently revealed that JAZ9 is not involved in most of the gene downregulation caused by COR, and the degradation of JAZ9-induced defense without inhibiting growth.

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Section: Resultsmentioning

confidence: 99%

Subtype-selective agonists of plant hormone co-receptor COI1-JAZs identified from the stereoisomers of coronatine

et al. 2023

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“…All parameters were by default. The final binding conformations were recorded based on the calculated GlideScore and the results were visualized using PyMOL ( 29 ).…”

Section: Methodsmentioning

confidence: 99%

Study on the binding behavior and functional properties of soybean protein isolate and β-carotene

et al. 2022

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This study focused on the non-covalent interaction between soybean protein isolate (SPI) and β-carotene (BC). The conformational changes of SPI with β-carotene in varying proportions (BC/SPI: 2%, 4%, 6%, 8%, and 10%) were investigated by multi-spectroscopy and molecular docking. Results showed that the quenching mode is static quenching and binding affinity increased with temperature. The stoichiometry was 1:1, indicating there was only one binding site in SPI. The binding was based on entropy and primarily driven by hydrophobic interactions and its binding constant was in the order of 104 L⋅mol–1. The addition of the β-carotene affected the secondary structure of SPI resulting in an increase in α-Helix and a decrease in random coil and β-turn content, indicating protein aggregated and hydrophobic interactions occurred. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) verified that no new larger molecular weight substance was formed and no covalent interaction existed. Molecular docking corroborated that electrostatic and hydrophobic interactions were both involved in the formation of complexes, where hydrophobic interaction was the dominant one. Moreover, β-carotene improved 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, foaming capacity, and emulsifying stability of SPI. These findings provide useful information about the interaction mechanism of SPI and β-carotene, which contributes to the further development and application of SPI products rich in β-carotene in the food industry.

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Small molecule chemical scaffolds in plant growth regulators for the development of agrochemicals

Dai,

Zheng,

Duan

et al. 2024

Chinese Chemical Letters

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Exploring the interaction mechanism between antagonist and the jasmonate receptor complex by molecular dynamics simulation

Cited by 6 publications

References 57 publications

Subtype-selective agonists of plant hormone co-receptor COI1-JAZs identified from the stereoisomers of coronatine

Subtype-selective agonists of plant hormone co-receptor COI1-JAZs identified from the stereoisomers of coronatine

Study on the binding behavior and functional properties of soybean protein isolate and β-carotene

Small molecule chemical scaffolds in plant growth regulators for the development of agrochemicals

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