Exploring the inside details of virions by electron microscopy

Liu, Zheng; Zhang, Jingqiang

doi:10.1007/s41048-016-0022-7

Cited by 1 publication

(1 citation statement)

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“…It should be noted herein that the previously determined K d for the homo-complex formation of pCRP is about 20μM[ 30 ], which is far below the present affinity. Indeed, the homogeneity of pCRP in solution phase under mild condition was further established by size-exclusion chromatography (SEC)[ 31 ] ( Fig 3D ) and electron microscope (EM)[ 32 – 34 ] ( Fig 3E ). SEC analysis of pCRP showed a single peak, and no apparent self-association of CRP like decamer was observed by EM.…”

Section: Resultsmentioning

confidence: 99%

In vitro generation and bioactivity evaluation of C-reactive protein intermediate

Wang

2018

PLoS ONE

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The conformational conversion of pentameric C-reactive protein (pCRP) to monomeric CRP (mCRP) has been shown to play important roles in the action of CRP in inflammation regulation. In vivo studies revealed the origin of mCRP and provided insights into how pCRP dissociation affected its functions. However, the interplay and exact bioactivities of CRP isoforms still remain uncertain due to the rapid conformational conversion and complex milieu in vivo. Herein, we have used surface-immobilization of pCRP to generate a preservable intermediate with dual antigenicity expression of both pCRP and mCRP. The intermediate has been further shown to exhibit modified bioactivities, such as a high affinity with solution-phase pCRP and an enhanced capacity of complement interaction. These results thus not only provide the conformational conversion details of CRP, but also propose a simple way in vitro to study how the functions of CRP are tuned by distinct isoforms.

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Section: Resultsmentioning

confidence: 99%

In vitro generation and bioactivity evaluation of C-reactive protein intermediate

Wang

2018

PLoS ONE

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Exploring the inside details of virions by electron microscopy

Cited by 1 publication

References 25 publications

In vitro generation and bioactivity evaluation of C-reactive protein intermediate

In vitro generation and bioactivity evaluation of C-reactive protein intermediate

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