Exploring the In Vitro Protective Effects of Green-Lipped Mussel (GLM) Oil Extract against Biomarkers of Glucose Metabolism and Inflammation in Chondrocyte Cells

Abstract

Chondrocyte behavior is critical in osteoarthritis (OA) progression and cartilage deterioration. Recent studies have shown that green-lipped mussel (GLM) oil extracts obtained through solvent extraction can regulate cytokine secretion in cell lines, potentially reducing inflammation and immune cell activation. This study investigates the effects of these extracts on human chondrocyte cell lines to understand their impact on osteoarthritis development. Chondrocyte cultures exposed to osteoarthritis-related inflammatory factors were treated with various concentrations of GLM oil. The results revealed that mussel oils, particularly a commercial mussel powder extract (SOLV.COM), enhance glucose uptake and protect chondrocyte cells. SOLV.COM effectively manages the release of inflammatory markers like interleukin-6 (IL-6) and matrix metalloproteinases-3 (MMP-3), showing increased deoxyglucose uptake. This study highlights the significant influence of extract choice on managing interleukin-6 (IL-6) secretion and cellular activation pathways, demonstrating the potential of SOLV.COM in managing osteoarthritis by controlling reactive oxygen production, regulating glucose metabolism, and inflammatory markers.

Investigation of the In Vitro Immunomodulatory Effects of Extracts from Green-Lipped Mussels (Perna canaliculus)

“…According to a recent study, GLM oil extract has the potential to reduce inflammation, enhance glucose uptake, and effectively manage the release of inflammatory markers, namely, IL-6 and MMP-3, on human chondrocyte cell lines. This highlights the promising role of SOLV.COM in controlling reactive oxygen production, regulating glucose metabolism, and managing OA [40].…”

“…However, after 48 h, cell viability was impacted at higher dilutions compared with ethanol, possibly due to a proliferation-activating effect. The highest dilution rate of crude oil samples (100 ug/mL) that did not significantly affect cell viability was selected for further experimentation, consistent with a previous in vitro study [40].…”